一株产L-谷氨酸氧化酶的链霉菌分离鉴定及酶学性质分析

生物技术通报 ›› 2012, Vol. 0 ›› Issue (12): 144-149.

一株产L-谷氨酸氧化酶的链霉菌分离鉴定及酶学性质分析

卢婵, 郑璞, 孙志浩

江南大学生物工程学院工业生物技术教育部重点实验室，无锡 214122

收稿日期:2012-06-04 修回日期:2013-01-25 出版日期:2012-12-26 发布日期:2013-02-06
作者简介:卢婵, 女, 硕士研究生, 研究方向:微生物菌种选育与发酵工艺;E-mail:doudou.opq123@163.com
基金资助:
: 国家“863”计划项目（2006AA020301-012）

Screening and Identification of a Strain Producing L-glutamateOxidase and Its Enzymatic Properties

Lu Chan, Zheng Pu, Sun Zhihao

The Key Laboratory of Industrial Biotechnology，Ministry of Education，School of Biotechnology，Jiangnan University，Wuxi 214122

Received:2012-06-04 Revised:2013-01-25 Published:2012-12-26 Online:2013-02-06

摘要/Abstract

摘要： 采用4-氨基安替吡啉/ 苯酚显色反应，从土壤中分离出产酶菌株Str-6。通过菌株形态、培养特征和生理生化试验、16S rDNA 序列分析，初步将其鉴定为山丘链霉菌。其初步酶学性质研究表明，该菌株所产L-谷氨酸氧化酶为胞外酶，28℃培养36 h 时产酶酶活达到0.023 1 U/mg 蛋白，对L-谷氨酸具有较强的底物专一性。酶作用最佳反应pH 为6.0，反应温度为35℃。在pH6.0-7.0 和温度35-40℃下具有较好的稳定性。

关键词: L-谷氨酸氧化酶, 筛选, 鉴定, 山丘链霉菌, 酶学性质

Abstract: Chromogenic 4-Aminoantipyrine/phenol reaction was used in the procedure of strain screening. A strain named Str-6 wasobtained. Taxonomy of the strain, based on its morphological, physiological and biochemical characteristics, as well as the sequence of 16SrDNA, was classified to Streptomyces collinus. The preliminary fermentation test showed that strain Str-6 could produced extracellular L-glutamateoxidase, and the maximal yield could reached 0.023 1 U/mg protein cultured at 28℃ for 36 h. It has strong substrate specificity for L-glutamate.The optimal pH was 6.0 and the temperature of the L-glutamate oxidase was 35℃ . The enzyme exhibited high thermal stability at 35-40℃ andstable at pH6.0--7.0.

卢婵, 郑璞, 孙志浩. 一株产L-谷氨酸氧化酶的链霉菌分离鉴定及酶学性质分析[J]. 生物技术通报, 2012, 0(12): 144-149.

Lu Chan, Zheng Pu, Sun Zhihao . Screening and Identification of a Strain Producing L-glutamateOxidase and Its Enzymatic Properties[J]. Biotechnology Bulletin, 2012, 0(12): 144-149.

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