一株具有IMPDH抑制活性的稀有放线菌的筛选和鉴定

生物技术通报 ›› 2012, Vol. 0 ›› Issue (12): 150-155.

一株具有IMPDH抑制活性的稀有放线菌的筛选和鉴定

袁丽杰^{1, 2}, 刘爱华¹, 张玉琴², 余利岩², 王茜¹, 张月琴²

1 河北联合大学基础医学院，唐山 063000 ；2 中国医学科学院医药生物技术研究所，北京 100050

收稿日期:2012-06-04 修回日期:2013-01-25 出版日期:2012-12-26 发布日期:2013-02-06
基金资助:
：科技部自然科技资源平台项目（2006DKA21203），河北省教育厅资助项目（2009466）

Screening and Identification of a Rare Actinomycetes ExhibitingIMPDH Inhibitive Activity

Yuan Lijie^1,2, Liu Aihua¹, Zhang Yuqin ², Yu Liyan², Wang Qian¹, Zhang Yueqin²

1 Hebei United University Medical College，Tangshan 063000 ；2 Institute of Medicinal Biotechnology，Chinese Academy of Medical Sciencesand Peking Union Medical College，Beijing 100050

Received:2012-06-04 Revised:2013-01-25 Published:2012-12-26 Online:2013-02-06

摘要/Abstract

摘要： 从重庆南川药用植物乌头根际土中分离到一株具有次黄嘌呤核苷磷酸脱氢酶（IMPDH）抑制活性的根际稀有放线菌株。通过菌株形态特征、培养特征观察、生理生化特征测定、细胞化学组分分析及16S rRNA 基因序列同源性分析等多相分类特征研究，确定I06-03147 属于动孢囊菌属（Kineosporia）的菌株。

关键词: 根际, 稀有放线菌, 筛选, 鉴定, 动孢囊菌属

Abstract: One actinomycete strain I06-03147 was isolated from rhizosphere soil of medicinal plant Aconitum carmichaeli Debx. collectedfrom Chongqing. The fermentation broth of strain I06-03147 exhibited inhibitive activity of IMPDH. In view of morphology, physiological andbiochemical characteristics, chemotaxonomic data, phylegentic analysis based on 16S rRNA gene sequence, the studied strain belonged to genusKineosporia.

袁丽杰, 刘爱华, 张玉琴, 余利岩, 王茜, 张月琴. 一株具有IMPDH抑制活性的稀有放线菌的筛选和鉴定[J]. 生物技术通报, 2012, 0(12): 150-155.

Yuan Lijie, Liu Aihua, Zhang Yuqin , Yu Liyan, Wang Qian, Zhang Yueqin. Screening and Identification of a Rare Actinomycetes ExhibitingIMPDH Inhibitive Activity[J]. Biotechnology Bulletin, 2012, 0(12): 150-155.

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