微生物转化生产S-腺苷甲硫氨酸

摘要/Abstract

摘要： S-腺苷甲硫氨酸（S-adenosyl-L-methionine，SAM）是具有广阔市场前景的活性氨基酸，微生物转化法是近年来报道较多的SAM 生产方法。综合近年来SAM 生产菌株的基因改造和发酵优化方面的进展，从提高SAM 合成酶表达和酶活、优化甲醇和甘油的流加方式、改善ATP 的生成和L-甲硫氨酸的补料、阻断下游代谢路径等方面，综述了促进SAM 合成及其积累的多重策略及机制。最后结合笔者多年研究实践，讨论了微生物转化生产SAM 的未来研究方向。

关键词: S-腺苷甲硫氨酸, 微生物转化, S-腺苷甲硫氨酸合成酶, 基因改造, 发酵优化

Abstract: S-adenosyl-L-methionine（SAM）is a type of active amino acid having broad market prospects，and there have been manyreports on SAM production catalyzed by microorganisms. This paper integrated advances in genetic modification and fermentation optimizationof SAM-producing strain. From the points of elevating expression and activity of SAM synthetase，optimizing feeding mode of glycerol andmethanol，improving the generation of ATP and supplement policy of L-methionine，and blocking downstream metabolic pathway，themultiple strategies of enhancing SAM synthesis and accumulation and their mechanisms had been reviewed. Furthermore，on the basis of theauthors’ research practice，some new prospects on SAM biosynthesis by microbes were discussed.

胡晓清, 郭雯, 韩国强. 微生物转化生产S-腺苷甲硫氨酸[J]. 生物技术通报, 2012, 0(12): 33-39.

Hu Xiaoqing, Guo Wen, Han Guoqiang . S-adenosyl-L-methionine Production by Microorganism[J]. Biotechnology Bulletin, 2012, 0(12): 33-39.

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