生物技术通报 ›› 2013, Vol. 0 ›› Issue (3): 171-174.

• 研究报告 • 上一篇    下一篇

一株拮抗放线菌T151的鉴定及其抑菌活性物质分析

李晓凤1,李淑英1,聂莹1,杜欢1,杨帆2,童大磊1,韦日清1,赵仲麟3,唐选明1   

  1. 1. 中国农业科学院农产品加工研究所,北京 100193 ; 2. 内蒙古农业大学,呼和浩特 010018 ; 3. 河南农业大学理学院,郑州 450002
  • 收稿日期:2012-09-29 修回日期:2013-03-21 出版日期:2013-03-20 发布日期:2013-03-21
  • 作者简介:李晓凤,女,硕士研究生,研究方向:生态学;E-mail :1061464181@qq.com

Preliminary Study for Antimicrobial Activity and Identification of Antagonistic Actinomyces T151

Li Xiaofeng1, Li Shuying1, Nie Ying1, Du Huan1, Yang Fan2, Tong Dalei1, Wei Riqing1, Zhao Zhonglin1, Tang Xuanming1   

  1. 1. Institute of Agro-products Processing Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193 ; 2. Inner Mongolia Agricultural University,Huhhot 010018 ; 3. College of Sciences,Henan Agricultural University,Zhengzhou 450002)
  • Received:2012-09-29 Revised:2013-03-21 Published:2013-03-20 Online:2013-03-21

摘要: 对一株从海南土壤样品中分离得到的放线菌T151 进行初步鉴定,分析其代谢产物的抑菌活性。通过扫描电镜形态 观察和16S rDNA 序列分析,初步鉴定该菌株为链霉菌属新种。抑菌活性分析表明,该菌发酵液对大肠杆菌、枯草芽孢杆菌和根霉 均有抑制作用;发酵液的稳定性分析表明活性成分在100℃处理30 min,pH5-11 仍保留很强的抑菌活性。

关键词: 放线菌, 16S rDNA, 链霉菌属, 抑菌活性

Abstract: Actinomyces T151, isolated from soil in Hainan province, was identified and screened for antimicrobial activity. T151 was designated as a new strain of Streptomyces according to the observation of SEM morpholiogical and analysis of 16S rDNA sequence. Its fermentation broth exhibited inhibition effect against Escherichia coli, Bacillus subtilis and Rhizopus. The fermentation broth was still owned high antimicrobial activity under 100℃ for 30 min or pH5-11.

Key words: Actinomyces, 16S rDNA, Streptomyces, Antimicrobial activity