马铃薯StSnRK2.1基因克隆与生物信息学分析

生物技术通报 ›› 2013, Vol. 0 ›› Issue (3): 83-89.

马铃薯StSnRK2.1基因克隆与生物信息学分析

刘思妍,毛娟,范阿棋,张俊莲,王蒂,白江平

甘肃省作物遗传改良与种质创新重点实验室甘肃省干旱生境作物学重点实验室甘肃农业大学农学院，兰州 730070

收稿日期:2012-09-18 修回日期:2013-03-21 出版日期:2013-03-20 发布日期:2013-03-21
作者简介:刘思妍，女，在读本科生，研究方向：农学专业；E-mail ：991630916@qq.com
基金资助:
国家科技支撑计划（2012BAD06B03），国家马铃薯产业技术体系，甘肃省科技重大专项计划（1102NKDA025），甘肃省财政厅农牧林业务专项资金，甘肃农业大学SRTP 科研项目（20120104）

Cloning and Bioinformation Analysiss of StSnRK2.1 Gene in Solanum tuberosum

Liu Siyan, Mao Juan, Fan Aqi, Zhang Junlian, Wang Di, Bai Jiangping

Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement,Gansu Provincial Key Laboratory of Aridland Crop Science,College of Agronomy,Gansu Agricultural University,Lanzhou 730070）

Received:2012-09-18 Revised:2013-03-21 Published:2013-03-20 Online:2013-03-21

摘要/Abstract

摘要： SnRK2 基因对植物的逆境胁迫具有重要的调节作用，以马铃薯‘陇薯3 号’（Solanum tuberosum）为试材，采用 RT-PCR 方法从马铃薯试管苗中克隆得到1 个SnRK2.1 基因cDNA，命名为StSnRK2.1，提交GenBank 注册，注册号为JX280911。通过生物信息学分析，该基因开放阅读框全长1 008 bp，编码335 个氨基酸。预测蛋白质分子量约为37.77 kD，等电点为5.37，蛋白质二级结构预测α-螺旋42.39%，延伸链16.42%，β-折叠7.46%，无规卷曲33.73%，具有疏水性，为膜内蛋白。亚细胞定位显示该基因出现在细胞质及微体中的可能性较大。肽链可能有7 处丝氨酸磷酸化位点，2 处苏氨酸磷酸化位点，以及3 处酪氨酸磷酸化位点，因此推测该基因在植物抗逆中有重要的作用。

关键词: 马铃薯, 抗旱, 基因克隆, 生物信息学分析

Abstract: SnRK2s plays an important role in the regulation when plants suffer from adervise environments. The full-length cDNA sequence of SnRK2.1 in Solanum tuberosum was successfully cloned by RT-PCR with the tube plant. The gene was named StSnRK2.1 and has been submitted to Genbank with the accession number JX280911.By bioinformation analysis, the results showed that the StSnRK2.1 has a complete ORF sequence is 1 008 bp, encoding 335 predicted amino acids. The protein molecular weight is 37.77 kD and theoretical pI is 5.37. In protein’s secondary structure, Alpha helix is 42.39%, Extended strand 16.42%, Bela turn is 7.46%, Random coil is 33.73%. Also, the protein is drophilicity, haven’t transmembrane. It is probably exit in the cytoplasm and microbody by subcellular localization prediction of protein. There are seven serine sites, two threonine sites, three tyrosine sites, so it is putative that the StSnRK2.1 is related to plant’s tolerance in abiotic stress.

Key words: Solanum tuberosum, Anti-drought, Cloning, Bioinformation analysis

刘思妍,毛娟,范阿棋,张俊莲,王蒂,白江平. 马铃薯StSnRK2.1基因克隆与生物信息学分析[J]. 生物技术通报, 2013, 0(3): 83-89.

Liu Siyan, Mao Juan, Fan Aqi, Zhang Junlian, Wang Di, Bai Jiangping. Cloning and Bioinformation Analysiss of StSnRK2.1 Gene in Solanum tuberosum[J]. Biotechnology Bulletin, 2013, 0(3): 83-89.

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