一株抗多重耐药菌海洋放线菌的筛选及其活性分析

生物技术通报 ›› 2013, Vol. 0 ›› Issue (7): 119-124.

一株抗多重耐药菌海洋放线菌的筛选及其活性分析

陈美群^1，2，3 冯广达² 邓名荣² 朱红惠²

（1.中国科学院南海海洋研究所，广州 510301；2.广东省微生物研究所广东省菌种保藏与应用重点实验室广东省华南应用微生物重点实验室——省部共建国家重点实验室培育基地广东省微生物应用新技术公共实验室，广州 510070；3.中国科学院研究生院，北京 100049）

收稿日期:2012-12-28 修回日期:2013-07-19 出版日期:2013-07-19 发布日期:2013-09-02
作者简介:陈美群，女，硕士研究生，研究方向：海洋放线菌的筛选及其活性产物分析；E-mail：cmq1986007@163.com
基金资助:
广东省科技攻关项目（2010B030800011）

Screening and Characterization of a Marine Actinomycete with Antagonist Activities Against Multidrug Resistant Bacteria

Chen Meiqun^1,2,3, Feng Guangda² Deng Mingrong² Zhu Honghui²

（1. South China Sea Institute of Oceanology，the Chinese Academy of Sciences，Guangzhou 510301；2. Guangdong Institute of Microbiology，Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application，State Key Laboratory of Applied Microbiology（Ministry-Guangdong Province Jointly Breeding Base），South China

Received:2012-12-28 Revised:2013-07-19 Published:2013-07-19 Online:2013-09-02

摘要/Abstract

摘要： 旨在从71株海洋放线菌中筛选活性较好的抗多重耐药菌菌株。采用平板打孔抑菌法筛选对多重耐药菌的抑制作用；通过菌株形态学特征、生理生化特征和16S rDNA序列对菌株进行分类鉴定。结果显示，筛选到一株对多株多重耐药菌均有较强抑制作用的海洋放线菌MQ-86，菌株MQ-86的M ₂⁺培养基粗提物对普通的大肠杆菌（Escherichia coli）8739、金黄色葡萄球菌（Staphylococcus aureus）6538、白色念珠菌（Candida albicans）10231和共耐18种主要抗生素的多重耐药性大肠杆菌（Escherichia coli）480、大肠杆菌（Escherichia coli）460、金黄色葡萄球菌（Staphylococcus aureus）206都有较好的抑制效果。菌株MQ-86对多株多重耐药菌均具有较强的抑制活性，经初步鉴定为小链霉菌（Streptomyces parvus）。

关键词: 海洋放线菌, 抑菌活性, 链霉菌属, 抗耐药菌

Abstract: It was to screening the antagonist activities against multidrug resistant bacteria from 71 marine actinomycetes. The inhibitory effect of 71 marine actinomycetes were detected against multidrug resistant bacteria by disk diffusion method, the strain was identified by morphological, physiological, biochemical characteristics and 16S rDNA sequence analysis. Results showed that strain MQ-86 showed strong inhibitory effect against multidrug resistant bacteria. The fermentation crude extract of strain MQ-86 using M₂₊ medium shows strong inhibitory effect to Escherichia coli 8739, Staphylococcus aureus 6538, Candida albicans 10231, Escherichia coli 480, Escherichia coli 460 and Staphylococcus aureus 206. Strain MQ-86 has a strong inhibitory effect against multidrug resistant organisms, which was identified as Streptomyces parvus.

Key words: Marine actinomycetes, Antibacterial activity, Streptomyces, Multidrug resistant bacteria

陈美群，冯广达,邓名荣,朱红惠. 一株抗多重耐药菌海洋放线菌的筛选及其活性分析[J]. 生物技术通报, 2013, 0(7): 119-124.

Chen Meiqun，Feng Guangda,Deng Mingrong,Zhu Honghui. Screening and Characterization of a Marine Actinomycete with Antagonist Activities Against Multidrug Resistant Bacteria[J]. Biotechnology Bulletin, 2013, 0(7): 119-124.

参考文献

[1] 焦炳华, 穆军, 许强芝, 缪辉南.海洋微生物来源新抗生素的研究[J].抗感染药学, 2004, 1（1）：1-9.
[2] Carte BK. Biomedical potential of marine natral producta [J]. Bioscience, 1996, 46（4）：271-286.
[3] J?rgensen BB, Boetius A. Feast and famine-microbial life in the deep-sea bed [J]. Nature Reviews Microbiology, 2001, 5：770-781.
[4] Bernan VS, Greenstein M, Carter GT. Mining marine microorganisms as a source of new antimicrobials and antifungals [J]. Curr Med Chem-Anti-Infective Agents, 2004, 3（3）：181-195.
[5] 林永成, 周世宁.海洋微生物及其代谢产物[M].北京：化学工业出版社, 2003.
[6] Imada C. Enzyme inhibitors and other bioactive compounds from marine actinomycetes [J]. Antonie van Leeuwenhoek, 2005, 87（1）：59-63.
[7] Cho JY, Williams PG, Kwon HC, et al. Lucentamycins A-D, cytotoxic peptides from the marine-derived actinomycete Nocardiopsis lucente-nsis [J]. Journal of Natural Products, 2007, 70（8）：1321-1328.
[8] Lam KS. Discovery of novel metabolites from marine actinomycetes [J]. Current Opinion in Microbiology, 2006, 9：245-251.
[9] 李富超.胶州湾两株海洋放线菌次级代谢产物的研究[D].北京：中国科学院研究生院, 2004.
[10] Lu YJ, Dong X, Liu S, Bie X. Characterization and identification of a novel marine Streptomyces sp. produced antibacterial substance [J]. Marine Biotechnology, 2009, 11（6）：717-724.
[11] Belofsky GN, Anguera M, Jensen PR, et al. Oxepinamides A-C and fumiquinazolines H-I：bioactive metabolites from a marine isolate of a fungus of the genus Acremonium [J]. Chemistry-A European Journal, 2000, 6（8）：1355-1360.
[12] Buchanan RE, Gibbons NE. 伯杰细菌鉴定手册[M].北京：科学出版社, 1984：729-795.
[13] 中国科学院微生物所放线菌分类组.链霉菌鉴定手册[M].北京：科学出版社, 1975：658-665.
[14] Weisburg WG, Barns SM, Pelletier DA. 16S ribosomal DNA amplification for phylogenetic study [J]. Journal of Bacteriology, 1991, 173（2）：697-703.
[15] Kumar S, Tamura K, Nei M. MEGA3：Integrated software or molecular evolutionary genetics analysis and sequence alignment [J]. Briefings in Bioinformatics, 2004, 5（2）：150-163.
[16] Monaghan RL, Barrett JF. Antibacterial drug discovery-then, now and the genomics future [J]. Biochemical Pharmacology, 2006, 71（7）：901-909.
[17] Nallapareddy SR, Duh RW, Singh KV, Murray BE. Molecular typing of selected Enterococcus faecalis isolates：pilot study using multilocussequence typing and pulsed-field gel electrophoresis [J]. Journal of Clinical Microbiology, 2002, 40（3）：868-876.
[18] Moellering RC Jr. NDM-1-a cause for worldwide concern [J]. N Enql J Med, 2010, 363（25）：2377-2379.
[19] Jensen PR, Mincer TJ, Williams PG, Fenical W. Marine actinomyc-ete diversity and natrual product discovery[J]. Antonie van Lee-uwenhoek, 2004, 87：43-48.
[20] 田新朋, 张偲, 李文均.海洋放线菌研究进展[J].微生物学报, 2011, 51（2）：161-169.
[21] Sivakumar K, Sahu MK, Thangaradjou T, Kannan L. Research on marine actinobacteria in India [J]. Indian Journal of Microbiology, 2007, 47：186-196.
[22] 孙建龙, 于基成, 刘志恒, 等.一株拮抗番茄溃疡病菌的海洋放线菌的分类鉴定及活性测定[J].吉林农业大学学报, 2012, 34（4）：391-394.
[23] 康银花, 王旻.抗MRSA小链霉菌NIM 521发酵工艺的优化[J].药物生物技术, 2008, 15（3）：208-211.
[24] 田兆丰, 刘伟成, 刘霆, 董丹.小链霉菌Yn168发酵产物抗植物病毒活性的研究[J].中国生物防治学报, 2011, 27（4）：569-572.