一株氨基半乳糖产生菌的筛选鉴定及培养条件研究

生物技术通报 ›› 2013, Vol. 0 ›› Issue (7): 125-130.

一株氨基半乳糖产生菌的筛选鉴定及培养条件研究

张荣岭王瑞明李丕武

（山东轻工业学院食品与生物工程学院山东省微生物工程重点实验室，济南 250353）

收稿日期:2013-03-08 修回日期:2013-07-19 出版日期:2013-07-19 发布日期:2013-09-02
作者简介:张荣岭，男，硕士研究生，研究方向：微生物酶技术；E-mail：zhangrongling86@126.com
基金资助:
国家“863“计划课题（2012AA021504）

Screening and Identification of a Galactosamine-producing Strain and Study of Its Cultural Conditions

Zhang Rongling, Wang Ruiming, Li Piwu

（Shandong Provincial Key Laboratory of Microbial Engineering，School of Food and Biological Engineering Shandong Institude of Light Industry，Ji’nan 250353）

Received:2013-03-08 Revised:2013-07-19 Published:2013-07-19 Online:2013-09-02

摘要/Abstract

摘要： 从东营黄河口湿地一处富含鱼蟹腐烂物的淤泥中分离筛选获得一株产氨基半乳糖的细菌YR06。根据形态特征、生理生化特征及16S rDNA序列分析，鉴定该菌株为嗜水气单胞菌。通过对该菌株摇瓶发酵进行的单因素试验和正交试验，优化得到培养基成分及培养条件：果糖10 g/L，尿素15 g/L，Na ₂HPO₄ 15 g/L，CaCl₂ 22.2 g/L，MgSO₄ 0.18 g/L，KH_{2PO₄ 5 g/L；发酵液初始pH6.5，装液量为60 mL/250 mL，温度为33℃，培养时间14 h。在此条件下氨基半乳糖产量达128 mg/L。}

关键词: 嗜水气单胞菌, 氨基半乳糖, 筛选, 鉴定, 培养条件

Abstract: A galactosamine producing-strain YR06 was isolated from the sludge where the dead fish and crab was decomposed in Dongying Yellow River estuary wetland. Based on morphology, physiological and biochemical characteristics as well as the 16S rDNA gene sequence, strain YR06 was identified as Aeromonas hydrophila. Optimization of YR06 fermentation conditions was studied via single factor and orthogonal test design. The optimum fermentations conditions were showed as followed：fructose 10 g/L, urea 15 g/L, Na₂HPO₄ 15 g/L, CaCl₂ 22.2 g/L, MgSO₄ 0.18 g/L, KH₂PO ₄ 5 g/L；initial pH6.5, fermentation holding 60 mL/250 mL, fermentation temperature 33℃. The yeild of galactosamine achieved 128 mg/L after the optimization.

Key words: Aeromonas hydrophila, Galactosamine, Screening, Identification, Cultural conditions

张荣岭,王瑞明,李丕武. 一株氨基半乳糖产生菌的筛选鉴定及培养条件研究[J]. 生物技术通报, 2013, 0(7): 125-130.

Zhang Rongling, Wang Ruiming, Li Piwu. Screening and Identification of a Galactosamine-producing Strain and Study of Its Cultural Conditions[J]. Biotechnology Bulletin, 2013, 0(7): 125-130.

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