中国恒河猴肠道菌群特征揭示

生物技术通报 ›› 2013, Vol. 0 ›› Issue (7): 153-160.

中国恒河猴肠道菌群特征揭示

赵娜^1，2 刘社兰³ 路纪琪⁴ 何宏轩² 赵宝华¹

（1.河北师范大学生命科学学院，石家庄 050016；2.中国科学院动物研究所，北京 100101；3.浙江省疾病预防与控制中心，杭州 310051；4.郑州大学生物工程系，郑州 450001）

收稿日期:2013-02-02 修回日期:2013-07-19 出版日期:2013-07-19 发布日期:2013-09-02
作者简介:赵娜，女，硕士研究生，研究方向：分子细菌学；E-mail：nazhao2007@163.com
基金资助:
中国科学院战略生物资源科技支撑体系运行专项（野生生物种质库 CZBZX-1）

Characterization of Intestinal Microbiota in Feces from Captive Healthy Rhesus Macaques

Zhao Na^1，2 Liu Shelan³ Lu Jiqi⁴ He Hongxuan² Zhao Baohua¹

（1. College of Life Science，Hebei Normal University，Shijiazhuang 050016；2. National Research Center for Wildlife Borne Diseases，Key Laboratory of Animal Ecology and Conservation Biology，Institute of Zoology，Chinese Academy of Sciences，Beijing 100101；3. Infectious Diseases Department，Zhejiang Center for Disease Control and Prevention，Hangzhou 310051；4. Institute of Biodiversity and Ecology，Zhengzhou University，Zhengzhou 450001）

Received:2013-02-02 Revised:2013-07-19 Published:2013-07-19 Online:2013-09-02

摘要/Abstract

摘要： 运用变性梯度凝胶电泳（DGGE）和实时荧光定量（q-PCR）技术分析6个年龄组恒河猴粪便样品中主要细菌情况，揭示肠道微生态特点。16S rRNA基因V3可变区PCR-DGGE指纹图谱显示条带丰富，割胶62条，克隆测序后与GenBank序列进行比对后构建进化树显示所有62个条带、43个OTUs属于4个门：厚壁菌门，放线菌门，拟杆菌门以及螺旋体门。PCR-DGGE图谱泳道相似性分析及主成分分析（PCoA）显示婴幼龄组和孕期组聚类明显。Q-PCR结果显示梭菌属细菌和双歧杆菌最为丰富，肠球菌属最少。梭菌属clusters ⅪⅤ，肠球菌属和拟杆菌-普氏菌属组间差异明显（P＜0.01）。作为显示肠道健康水平标准的双歧杆菌和肠杆菌比值（B/E值）与同龄恒河猴相比，在孕期恒河猴组有明显的降低（P<0.01）。婴幼龄组和孕期组特点相似属首次发现。

关键词: 中国恒河猴肠道菌群, 变性梯度凝胶电泳, 实时荧光定量

Abstract: With the denaturing gradient gel electrophoresis（DGGE）and real-time quantitative PCR（q-PCR）, we analyzed the predominant bacteria in fecal samples of 24 individuals divided into 6 groups. DGGE profiles showed abundant bands for the 16S rRNA gene V3 region, and the composition of the gut microbiota was apparently different in each group. Furthermore, a clustering between infant and pregnancy was shown by Principal Coordinate Analysis（PCoA）. Identified in the DGGE gels by sequencing the V3 regions were overwhelmingly affiliated with Firmicutes, Actinobacteria and Bacteroidetes. Q-PCR indicated that Clostridium and Bifidobacterium were the dominant species, and the least dominant species was Enterococcus faecali. C.clustersⅪⅤ, E.faecalis and Bacteroides-Prevotella group have the most significant diversity among individuals（P<0.01）The Bifidobacteria /Enterobacteriaceae（B/E）ratio, which indicates microbial colonization resistance（CR）in the gut appeared to be lower than the peers, subadults and adults, in pregnancy（P<0.01）. The study revealed the characterization of Rhesus Macaquesintestinal microbiota. The characteristics of infant gut microbiota are remarkably similar to those found of the pregnancy groups.

Key words: Rhesus macaques gut microbiota, DGGE Real-time quantitative, PCR

赵娜,刘社兰,路纪琪,何宏轩,赵宝华. 中国恒河猴肠道菌群特征揭示[J]. 生物技术通报, 2013, 0(7): 153-160.

Zhao Na,Liu Shelan,Lu Jiqi,He Hongxuan,Zhao Baohua. Characterization of Intestinal Microbiota in Feces from Captive Healthy Rhesus Macaques[J]. Biotechnology Bulletin, 2013, 0(7): 153-160.

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