绒山羊DBY基因的BAC筛选与序列分析

生物技术通报 ›› 2013, Vol. 0 ›› Issue (7): 89-93.

绒山羊DBY基因的BAC筛选与序列分析

肖红梅¹ 肖旭² 刘志红³ 李金泉³

（1.内蒙古农业大学生命科学学院，呼和浩特 010018；2.内蒙古师范大学基础教育学院，呼和浩特 010020；3.内蒙古农业大学动物科学学院，呼和浩特 010018）

收稿日期:2012-12-28 修回日期:2013-07-19 出版日期:2013-07-19 发布日期:2013-09-02
作者简介:肖红梅，女，博士研究生，副教授，研究方向：动物遗传育种与繁殖；E-mail：lhtdyx@126.com
基金资助:
国家自然科学基金资助项目（31072005）

Screening and Sequence Analysis of Cashmere Goat DBY

Xiao Hongmei¹ Xiao Xu² Liu Zhihong³ Li Jinquan³

（1. College of Life Science，Inner Mongolia Agricultural University，Hohhot 010018；2. College of General Education，Inner Mongolia Normal University，Hohhot 010020；3. College of Animal Science，Inner Mongolia Agricultural University，Hohhot 010018）

Received:2012-12-28 Revised:2013-07-19 Published:2013-07-19 Online:2013-09-02

摘要/Abstract

摘要： 绒山羊是我国一种独特的生物资源，是经过长期的自然选择和人工选育而成的目前世界上产绒量最高、绒纤维品质最好的品种，所以提高绒山羊的繁殖力，对经济的发展有着非常重要的意义。运用PCR技术，对绒山羊Y染色体上控制精子生成的DBY基因进行BAC文库筛选、克隆、鉴定、测序，得到240 bp的DBY基因片段。与GenBank收录的其他物种进行序列比对，结果显示，与绵羊序列的同源性达96%，与坡鹿、水牛的同源性达93%；与猪鹿、瘤牛和牛的同源性分别达92%和91%；而与人和黑猩猩及小鼠的序列无同源性，共编码52个氨基酸。物种间NJ的聚类结果与其在系统分类学的地位相一致。

关键词: 绒山羊, DBY, BAC, 序列分析

Abstract: Cashmere goat is a unique biological resource in China. After a long period of natural selection and artificial breeding, Cashmere goat has best fiber quality and higher cashmere yields at present in the world. Therefore, the high reproductive rate of Cashmere goat is essential for developing economy. In this experiment, 240 bp gene fragment was obtained, though screening from Cashmere goat BAC library, cloning, and identifying DBY gene controlling spermatogenesis on the Y chromosome by PCR. Then the sequence of Cashmere goat was compared with other species from GenBank. The results showed Cashmere goat has homology of 96% with Ovis aries, homology of 93% with Cervus eld and Bubalus bubalis. The homology is up to 92% and 91% with Axis porcinus, Bos indicus and Bos teurus, respectively, no homology with the human, chimpanzee and mouse. The sequence fragment encodes 52 amino acids. The clustering consequence of species NJ consistent with the taxonomic status.

Key words: Cashmere goat, DBY, BAC, Sequence analysis

肖红梅,肖旭,刘志红,李金泉. 绒山羊DBY基因的BAC筛选与序列分析[J]. 生物技术通报, 2013, 0(7): 89-93.

Xiao Hongmei,Xiao Xu,Liu Zhihong,Li Jinquan. Screening and Sequence Analysis of Cashmere Goat DBY [J]. Biotechnology Bulletin, 2013, 0(7): 89-93.

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