生物技术通报 ›› 2013, Vol. 0 ›› Issue (8): 74-77.

• 研究报告 • 上一篇    下一篇

海南木莲遗传多样性的ISSR及亲缘关系的分析

魏小玲, 曹福祥, 陈建   

  1. (中南林业科技大学,长沙 410004)
  • 收稿日期:2013-03-18 修回日期:2013-08-11 出版日期:2013-08-11 发布日期:2013-09-02
  • 作者简介:魏小玲,女,硕士研究生,研究方向:生物化学与分子生物学;E-mail:csftu-wxl@163.com
  • 基金资助:
    国家林业公益行业科研专项子项目(200804001)

Studies on the Genetic Diversity and Relationship of Manglietia hainanensis Dandy by ISSR

Wei Xiaoling, Cao Fuxiang, Chen Jian   

  1. (Central South University of Forestry and Technology,Changsha 410004)
  • Received:2013-03-18 Revised:2013-08-11 Published:2013-08-11 Online:2013-09-02

摘要: 将简单重复序列区间扩增多态标记(inter-simple sequence repeats,ISSR)技术应用于海南木莲4个种群的36份样品的遗传多样性分析,10个引物共扩增出106条条带,其中有87条多态性条带,多态位点百分率(PPB)为82.08%。4个种群的海南木莲等位基因数(Ne)的变异幅度为1.416 6-1.625 7,Nei's基因多样性指数(H)的变异幅度为0.222 8-0.339 2,Shannon信息指数(I)的变异幅度为0.315 1-0.491 0,种群间的分化系数(Gst)为0.197 1,由遗传一致度进行了4个种群的UPGMA聚类。

关键词: 海南木莲, ISSR, 遗传多样性, 亲缘关系

Abstract: Inter-simple sequence repeat(ISSR)technique was applied to detect 4 populations and 36 samples of Manglietia hainanensis Dandy,and 106 bands were amplied by 10 primers,including 87 polymrphic bands. The polymorphic percentage is 82.08%. The variation range of alleles per locus(Ne)was 1.416 6-1.625 7;variation range of Nei's gene diversity(H)was 0.222 8-0.339 2;variation range of shannon's information index(I)was 0.315 1-0.491 0;coefficient of gene differentiation(Gst)of the 4 populations was 0.197 1. Based on the genetic identity,dendrogram of 4 populations was constructed by UPGMA.

Key words: Manglietia hainanensis, Dandy, ISSR, Genetic diversity, Relationship