生物技术通报 ›› 2013, Vol. 0 ›› Issue (10): 103-108.

• 研究报告 • 上一篇    下一篇

7号染色体46765080位点SNP与绵羊尾臀性状相关性研究

梁耀伟,张伟,沈敏,李欢,高磊,杨井泉,刘守仁,甘尚权,王新华   

  1. (新疆农垦科学院畜牧兽医研究所,石河子 832000)
  • 收稿日期:2013-08-05 修回日期:2013-10-15 出版日期:2013-10-14 发布日期:2013-10-15
  • 作者简介:梁耀伟, 男, 助理研究员, 研究方向: 动物分子遗传学;E-mail :lyw_ld123456@163.com
  • 基金资助:

    “973” 前期专项项目(2011CB111501), 国家自然科学基金新疆联合基金重点项目(U1130302)

The Correlation Between a Novel SNP on Chromosome 7 and the Trait of Sheep Tail or Rump

Liang Yaowei, Zhang Wei, Shen Min, Li Huan, Gao Lei, Yang Jingquan, Liu Shouren, Gan Shangquan, Wang Xinhua   

  1. (Institute of Animal Husbandry and Veterinary Medicine,Xinjiang Academy of Agricultural and Reclamation Sciences,Shihezi 832000)
  • Received:2013-08-05 Revised:2013-10-15 Published:2013-10-14 Online:2013-10-15

摘要:

绵羊尾(臀)脂性状是重要耐逆性状,其脂肪沉积的分子机制不清。旨在研究绵羊脂尾(臀)沉积脂肪的分子遗传机制,以最近文献报道的7 号染色体一处SNP 位点为候选分子标记,利用PCR-RFLP 方法检测该位点在尾型极端差异的阿勒泰羊、哈萨克羊、湖羊、中国美利奴细毛羊以及萨福克羊群体中的多态性,并采用模型分析其与尾(臀)脂性状的相关性。结果表明, 7 号染色体46765080 位点的G 等位基因高频出现在表型分值较高的臀脂型阿勒泰羊群体中,A 等位基因在长瘦尾绵羊品种中高频出现;等位基因频率G/A 的比值与尾臀表型分值相关性模型表明G/A 比值随着尾臀表型分值增加呈指数增长。以上结果表明,绵羊7 号染色体46765080 位点在尾(臀)脂与瘦尾绵羊群体中分布存在显著性差异,该SNP 位点可作为一个理想的分子标记应用于高、低脂绵羊品种选育。

关键词: 耐逆性状, 脂尾性状, 阿勒泰羊, 多态性, 基因型

Abstract:

The fat-tailed or fat-rumped is one of important trait of stress tolerance, however, the mechanism of fat deposition is still unclear. Therefore, the present study choose a novel SNP reported in recently literature as a candidate molecular marker and detect the polymorphism among Altay, Kazakh, Hu, Chinese Merino and Suffolk which has extreme differences in tail type by using PCR-RFLP method. In addition, a mathematical model was employed to analyze the correlation between the polymorphism and the trait of fat-tailed or fat-rumped. Our results showed that the frequency of G allele was highest in Altay which had higher phenotype score, and the frequency of A allele was rather higher in thin-tailed sheep breeds. The result of mathematical model showed that the ratio of G/A increased exponentially with the phenotype score increasing. Together, the results suggested that there was significant differences in the SNP distribution between fat-tailed and thin-tailed sheep populations, and the SNP could be used as an ideal molecular marker to help high and low fat sheep breeding.

Key words: Stress tolerance, Fat tail trait, Altay sheep, Polymorphism, Genotype