香蕉ASR 的特征和采后表达分析

生物技术通报 ›› 2014, Vol. 0 ›› Issue (1): 105-111.

香蕉ASR 的特征和采后表达分析

贾彩红¹,徐碧玉¹,刘菊华¹,张建斌¹,冯仁军¹,金志强²

1. 中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室，海口 570101
2. 中国热带农业科学院海口试验站海南省香蕉遗传改良重点实验室，海口 570101

收稿日期:2013-10-16 出版日期:2014-01-23 发布日期:2014-01-23
作者简介:贾彩红，女，副研究员，硕士，研究方向: 香蕉分子生物学；E-mail: jiach9@163.com
基金资助:
国家自然科学基金项目(31071788)，‘ 十二五’ 农村领域国家科技计划课题(2011AA10020605)，现代农业产业技术体系建设专项资金资助项目(CARS-32)

Characteristic and Expression Analysis of ASR Gene from Banana

Jia Caihong¹, Xu Biyu¹, Liu Juhua¹, Zhang Jianbin¹, Feng Renjun¹, Jin Zhiqiang²

1. Key Laboratory of Biology and Genetic Resources of Tropical Crops，Ministry of Agriculture，Haikou 570101;
2. Haikou Experimental Station，Institute of Banana，Chinese Academy of Tropical Agricultural Sciences，Haikou 570101

Received:2013-10-16 Published:2014-01-23 Online:2014-01-23

摘要/Abstract

摘要： 从香蕉cDNA 文库中获得香蕉ASR 全长cDNA 序列，命名为MaASR。构建遗传进化树发现MaASR 与单子叶植物中#br#的芭蕉科植物的亲缘关系较近。对正常成熟和乙烯处理的香蕉果实各时期内源ABA 的含量进行测定，结果表明，在正常成熟的香#br#蕉果实中，成熟早期(0-2 d)，内源ABA 含量相对较低，第8 天ABA 含量达到最大，随后迅速下降。在乙烯处理的香蕉果实中，#br#ABA 含量急剧上升，第3 天ABA 含量达到高峰，随后逐渐下降。利用荧光定量RT-PCR 对MaASR 在正常成熟和乙烯处理后的果#br#实中的表达情况进行分析，在自然成熟的果实中，MaASR 在采后2 d 达到高峰，而后迅速下降，在6-16 d 时一直保持较低水平。#br#在经外源乙烯诱导的果实中，MaASR 表达水平没有表现出剧烈变化的趋势，整体表达量较低。表明MaASR 对乙烯信号有应答，但#br#应答强度较低，其表达趋势与ABA 的含量或者信号强度呈负相关。

关键词: 香蕉, ASR, 特征表达分析

Abstract: ASR gene was obtained from banana cDNA libraries in this study and ws named as MaASR. Phylogenetic analysis revealed MaASR was close relatives to Musaceae of Monocotyledons. Endogenous ABA content was determined in naturally ripened bananas and ethylenetreated bananas. In naturally ripened bananas, endogenous ABA content is relatively low at mature early(0-2 d), and increase maximum at 8 day, then drop rapidly. In ethylene- treated bananas, ABA content rise sharply peak at 3 days and then gradually decline. Real-time quantitative PCR analysis expression of MaASR in naturally ripened bananas and ethylene- treated bananas. In naturally ripened bananas, MaASR expression increased and peaked 2 days after harvest, then fell rapidly and remained low at 6-16 d. While in ethylene- treated bananas, MaASR expression levels do not show the dramatic change trend, the expression is overall low. The results showed that MaASR was responding to ethylene signal,but response intensity is low, its expression trend was negatively correlated with the content of ABA or signal strength.

Key words: Banana, ASR gene, Characteristic Expression analysis

贾彩红,徐碧玉,刘菊华,张建斌,冯仁军,金志强. 香蕉ASR 的特征和采后表达分析[J]. 生物技术通报, 2014, 0(1): 105-111.

Jia Caihong, Xu Biyu, Liu Juhua, Zhang Jianbin, Feng Renjun, Jin Zhiqiang. Characteristic and Expression Analysis of ASR Gene from Banana[J]. Biotechnology Bulletin, 2014, 0(1): 105-111.

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