木薯MeASR基因克隆及表达分析

doi:10.13560/j.cnki.biotech.bull.1985.2015.10.021

生物技术通报 ›› 2015, Vol. 31 ›› Issue (10): 125-130.doi: 10.13560/j.cnki.biotech.bull.1985.2015.10.021

木薯MeASR基因克隆及表达分析

胡伟,颜彦,韦运谢,王文泉,夏志强,卢诚,侯晓婉,彭明

中国热带农业科学院热带生物技术研究所, 海口 571101

收稿日期:2015-02-02 出版日期:2015-10-28 发布日期:2015-10-28
作者简介:胡伟, 男, 助理研究员, 研究方向：植物分子生物学; E-mail：huwei2010916@126.com
基金资助:
中央级公益性科研院所基本科研业务费（1630052014003, ITBB140204）, 海南省自然科学基金项目（314122）, 海南省重大科技专项（ZDZX2013023-1）

Clone and Expression of MeASR Gene in Cassava

Hu Wei, Yan Yan, Wei Yunxie, Wang Wenquan, Xia Zhiqiang, Lu Cheng, Hou Xiaowan, Peng Ming

Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou 571101

Received:2015-02-02 Published:2015-10-28 Online:2015-10-28

摘要/Abstract

摘要： 脱落酸-胁迫-成熟诱导蛋白（Abscisic acid-stress-ripening, ASR）在植物对非生物逆境胁迫的应答过程中发挥着重要作用。利用PCR技术从木薯中克隆了第一个ASR基因MeASR, 序列分析表明该基因开放阅读框（ORF）330 bp, 编码109个氨基酸。多序列比对和进化树分析表明该基因所编码的蛋白具有ASR家族蛋白的保守结构域, 与番茄ASR家族蛋白SlASR4具有较近的亲缘关系。亚细胞定位分析表明MeASR定位在细胞核, 实时荧光定量PCR分析表明该基因的表达显著受渗透胁迫和ABA诱导。结果表明, MeASR可能作为转录因子参与木薯对干旱逆境胁迫应答及ABA信号调节。

关键词: 木薯, MeASR基因, 基因克隆, 表达分析, 亚细胞定位

Abstract: Abscisic acid-stress-ripening induced protein（ASR）plays an important role in response to abiotic stresses. In the present study we isolated a first ASR gene designated MeASR from cassava. Sequence analysis showed that the open reading frame of MeASR gene contained 330 bp, encoding 109 amino acids. Multiple sequence alignment and phylogenetic analysis indicated that MeASR protein contained the conserved domains as ASR family had, and had a close genetic relationship with SlASR4 of tomato. Subcellular location assay showed that MeASR protein was localized in nucleus. Real-time quantitative PCR assay revealed that expression of MeASR was induced by osmotic stress and ABA treatments. These results suggested that MeASR might function as a transcription factor to involve in response to drought stress and ABA-signal regulation in cassava.

Key words: cassava, MeASR gene, gene clone, expression analysis, subcellular location

胡伟,颜彦,韦运谢,王文泉,夏志强,卢诚,侯晓婉,彭明. 木薯MeASR基因克隆及表达分析[J]. 生物技术通报, 2015, 31(10): 125-130.

Hu Wei, Yan Yan, Wei Yunxie, Wang Wenquan, Xia Zhiqiang, Lu Cheng, Hou Xiaowan, Peng Ming. Clone and Expression of MeASR Gene in Cassava[J]. Biotechnology Bulletin, 2015, 31(10): 125-130.

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木薯MeASR基因克隆及表达分析

Clone and Expression of MeASR Gene in Cassava

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