柴油高效降解菌株筛选及降解特性研究

生物技术通报 ›› 2014, Vol. 0 ›› Issue (1): 161-165.

柴油高效降解菌株筛选及降解特性研究

岳贵龙,陈亮,刘娜

河南工业大学生物工程学院，郑州 450001

收稿日期:2013-11-07 出版日期:2014-01-23 发布日期:2014-01-23
作者简介:岳贵龙，男，硕士研究生，研究方向: 应用微生物；E-mail: yueguilong@163.com
基金资助:
国家自然科学基金项目(U1304332)，河南工业大学引进人才专项基金资助项目(2009BS052)，河南工业大学校科学研究基金研究生教育创新计划项目(2012YJCX49)

Screening of Diesel-degrading Strains and Their Degrading Characteristics

Yue Guilong, Chen Liang, Liu Na

College of Bioengineering，Henan University of Technology，Zhengzhou 450001

Received:2013-11-07 Published:2014-01-23 Online:2014-01-23

摘要/Abstract

摘要： 采用梯度富集培养、稀释涂布从受石油污染的样品中，分离得到柴油降解菌株10 株，其中菌株YR2 柴油降解率最高，#br#在含柴油1%(w/v)的无机盐液体培养基中培养7 d，降解率达到92.8%，在2%、4%、5% 的柴油浓度下降解率分别为60.8%、#br#53.5%、41.0%。综合菌株形态特征观察、生理生化特性分析和16S rDNA 序列比对，菌株YR2 应为铜绿假单胞菌(Pseudomonas#br#aeruginosa)。菌株YR2 具有较好的细胞表面疏水性、乳化性能和排油性能。薄层层析结果表明菌株YR2 分泌糖脂类表面活性剂。#br#菌株YR2 具有高效的柴油降解能力，有望应用于柴油污染的微生物修复。

关键词: 柴油, 假单胞菌, 糖脂, 细胞表面疏水性, 微生物降解

Abstract: Ten diesel-degrading strains were isolated from oil-contaminated samples by gradient enrichment culture and dilution coating. Strain YR2 with highest diesel degradation ability was chosen for further study. The degradation rate of strain YR2 was 92.8% when it was inoculated in mineral medium with diesel concentration of 1 g/100 mL for 7 days. The degradation rate was respectively 60.8%, 53.5% and 41.0% when the diesel concentration was 2 g/100 mL, 4 g/100 mL and 5 g/100 mL. Based on morphological, physiological-biochemical features and 16S rDNA sequences comparison, strain YR2 was identified as Pseudomonas aeruginosa. Strain YR2 has the excellent cell surface hydrophobicity, emulsifying ability and oil displacement ability. Strain YR2 can produce glycolipid biosurfactant. Strain YR2 with efficient diesel degradation ability has the potential application in the microbial remediation of diesel pollution.

Key words: Diesel, Pseudomonas sp., Glycolipid, Cell surface hydrophobicity, Microbial degradation

岳贵龙,陈亮,刘娜. 柴油高效降解菌株筛选及降解特性研究[J]. 生物技术通报, 2014, 0(1): 161-165.

Yue Guilong, Chen Liang, Liu Na. Screening of Diesel-degrading Strains and Their Degrading Characteristics[J]. Biotechnology Bulletin, 2014, 0(1): 161-165.

参考文献

[1] 禄立彦, 邓超, 段作营, 等. 两株柴油降解菌的筛选和降解特性研究[J]. 工业微生物, 2012, 42(6): 14-20.
[2] Lee M, Kim MK, Kwon MJ, et al. Effect of the synthesized mycolic acid on the biodegradation of diesel oil by Gordonia nitida strain LE31[J]. Journal of Bioscience and Bioengineering, 2005, 100(4): 429-436.
[3] 马晓焉, 陈德育, 段敏, 等. 柴油降解细菌的筛选及生长特性初探[J]. 西北农林科技大学学报, 2013, 41(6): 85-90.
[4] Lee M, Woo SG, Ten LN. Characterization of novel diesel-degrading strains Acinetobacter haemolyticus MJ01 and Acinetobacter johnsonii MJ4 isolated from oil-contaminated soil[J]. World Journal of Microbiology and Biotechnology, 2012, 28(5): 2057-2067.
[5] Huang L, Ma T, Li D, et al. Optimization of nutrient component for diesel oil degradation by Rhodococcus erythropolis[J]. Marine Pollution Bulletin, 2008, 56(10): 1714-1718.
[6] 沈萍, 范秀容, 李广武. 微生物学实验[M]. 第3 版. 北京: 高等教育出版社, 1999: 50-51.
[7] 国家环境保护总局《水和废水监测分析方法》编委会. 水和废水监测分析方法[M]. 第4 版. 北京: 中国环境科学出版社,2002: 490-491.
[8] 东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京: 科学出版社, 2001: 162-167.
[9] 李静琴, 陈亮, 岳贵龙, 等. 冬枣储藏期致腐真菌分离鉴定及广谱拮抗菌株的筛选[J]. 河南工业大学学报: 自然科学版,2013, 34(2): 62-66.
[10] 高小朋, 姜钊, 高秀梅, 等. 石油降解菌产表面活性剂的条件优化[J]. 环境工程学报, 2013, 7(8): 3244-3248.
[11] Babita K, Singh SN, Singh DP. Characterization of two biosurfactant producing strains in crude oil degradation[J]. Process 2014年第1期165 岳贵龙等: 柴油高效降解菌株筛选及降解特性研究 Biochemistry, 2012, 47(12): 2463-2471.
[12] Hou D, Shen X, Luo Q, et al. Enhancement of the diesel oil degradation ability of a marine bacterial strain by immobilization on a novel compound carrier material[J]. Marine Pollution Bulletin,2013, 67(1-2): 146-151.
[13] 孙敏, 沈先荣, 侯登勇, 等. 高效柴油降解菌Acinetobacter W3 分离鉴定及降解酶基因扩增分析[J]. 生物技术通报, 2012 (6): 159-164.
[14] 田胜艳, 刘廷志, 高秀花, 等. 海洋潮间带石油烃降解菌的筛选分离与降解特性[J]. 农业环境科学学报, 2006, 25: 301- 305.
[15] Lee M, Kim MK, Singleton I., et al. Enhanced biodegradation of diesel oil by a newly identified Rhodococcus baikonurensis EN3 in the presence of mycolic acid[J]. Journal of Applied Microbiology, 2006, 100(2): 325-333.
[16] Nikaido H. Molecular basis of bacterial outer membrane permeability revisited[J]. Microbiology and Molecular Biology Reviews, 2003, 67(4): 593-656.
[17] 赵晴, 张甲耀, 陈兰洲, 等. 疏水性石油烃降解菌细胞表面疏水性及降解特性[J]. 环境科学, 2005, 26(5): 132-136.
[18] Najafi AR, Rahimpour MR, Jahanmiri AHR, et al. Enhancing biosurfactant production from an indigenous strain of Bacillus mycoides by optimizing the growth conditions using a response surface methodology[J]. Chemical Engineering Journal, 2010,163(3): 188-194.