PFOS对东亚三角涡虫HSP70蛋白及基因表达的影响

摘要/Abstract

摘要： 全氟辛烷磺酸（Perfluorooctane sulfonate,PFOS）是一种持续的有机污染物,是全氟化合物（Perfluorinated chemicals,PFCs）在环境中的代表性物质。以低等三胚层动物涡虫作为试验材料,使用Western blot及RT-PCR技术探究PFOS对涡虫HSP70蛋白及基因表达的影响。结果表明,PFOS短时间处理能诱导涡虫hsp70 mRNA表达量升高,涡虫再生10 d mRNA表达受到抑制,HSP70蛋白表达量在涡虫再生4 d和7 d时随PFOS浓度显著上升,其它组变化不明显,PFOS对mRNA和蛋白表达量趋势影响不一致,可能少量PFOS能抑制HSP70蛋白翻译,药物在涡虫体内积累量增加,蛋白高表达抵御毒性伤害。

关键词: PFOS, 三角涡虫, HSP70, RT-PCR, Western blot

Abstract: Perfluorooctane sulfonate（PFOS）is a persistent organic pollutant and has been found to be the predominant perfluorinated chemicals（PFCs）in the environment. In this study, we used planarians Dugesia japonica, which belong to the phylum Platyhelminthes, class Turbellaria, as an animal assay to evaluate the toxicological effects of PFOS on protein and mRNA expression of HSP70. The results indicated that the expression of hsp70 mRNA of planarians increased exposed to PFOS in short time and decreased when planarians regenerated of 10-days. However, protein expression of HSP70 increased significantly after regenerating planarians exposed to PFOS for 4- and 7-days, and other groups were not influenced significantly. The trend of mRNA expression were inconsistent with protern. It may be that little PFOS would inhibit the translation of HSP70, and the number of HSP70 increased to protect from toxicity of PFOS as that was accumulated in planarians.

Key words: PFOS, Planatian, HSP70, RT-PCR, Western blot

宫晓宁, 袁佐清, 白芸. PFOS对东亚三角涡虫HSP70蛋白及基因表达的影响[J]. 生物技术通报, 2014, 0(10): 156-160.

Gong Xiaoning, Yuan Zuoqing, Bai Yun. Effects of PFOS on Expression of HSP70 in Planarian Dugesia japonica[J]. Biotechnology Bulletin, 2014, 0(10): 156-160.

参考文献

[1] Sinclair E, Mayack DT, Roblee K, et al. Occurrence of perfluoroalkyl surfactants in water, fish, and birds from New York State[J]. Arch Environ Contam Toxicol, 2006, 50（3）：398-410.
[2] YH, Liu W, Sato I, et al. PFOS and PFOA in environmental and tap water in China[J]. Chemosphere, 2009, 77（5）：605-611.
[3] G, You C. Sediment-water distribution of perfluorooctane sulfonate（PFOS）in Yangtze River Estuary[J]. Environmental Pollution, 2010, 158（5）：1363-1367.
[4] Houde M, Martin JW, Letcher RJ, et al. Biological monitoring of polyfluoroalkyl substances：a review[J]. Environmental Science & Technology, 2006, 40：3463-3473.
[5] Luebker DJ, Hansen KJ, Bass NM, et al. Interactions of fluorochemi-cals with rat liver fatty acid-binding protein[J]. Toxicology, 2002, 176（3）：175-185.
[6] ME, Clewell HJ, Tan YM, et al. Pharmacokinetic modeling of saturable, renal resorption of perfluoroalkylacids in monkeys-probing the determinants of long plasma half-lives[J]. Toxicology, 2006, 227（1-3）：156-164.
[7] Anne EL, Jerry LC, John LB, et al. Evaluation of placental and lactational pharmacokinetics of PFOA and PFOS in the pregnant, lactating, fetal and neonatal rat using a physiologically based pharmacokinetic model[J]. Reproductive Toxicology, 2012, 33（4）：468-490.
[8] LN, Katherine KC, Susan AB, et al. Effects of perfluorooctane sulfonate on mallard and northern bobwhite quail exposed chronically via the diet[J]. Environmental Toxicology and Pharmacology, 2007, 23（1）：1-9.
[9] KT, Raaschou-Nielsen O, Sørensen M, et al. Genotoxic potential of the perfluorinated chemicals PFOA, PFOS, PFBS, PFNA and PFHxA in human HepG2 cells[J]. Mutation Research, 2010, 700（1-2）：39-43.
[10] Florentin A, Deblonde T, Diguio N, et al. Impacts of two peruorinated compounds（PFOS and PFOA）on human hepatoma cells：Cytotoxicity but no genotoxicity?[J]. International Journal of Hygiene and Environmental Health, 2011, 214（6）：493-499.
[11] W, Ludewig G, Wang K, et al. Model and cell membrane partitioning of perfluorooctanesulfonate is independent of the lipid chain length[J]. Colloids and Surfaces B：Biointerfaces, 2010, 76（1）：128-136.
[12] WY, Jones PD, Celius T, et al. Identification of genes responsive to PFOS using gene expression profiling[J]. Environmental Toxicology and Pharmacology, 2005, 19（1）：57-70.
[13] N, Todgham AE, Ackerman PA, et al. Heat shock protein genes and their functional significance in fish[J]. Gene, 2002, 295（2）：173-183.
[14] ZQ, Zhao BS, Zhang JY, Zhang SC. Characterization and expression of djpreb Gene in the planarian Dugesia jiaoniea[J]. Molecular Biology, 2010, 44（l）：8-13.
[15] WY, Jones PD, Coen WD, et al. Comparison of gene expression methods to identify genes responsive to perfluorooctane sulfonic acid[J]. Environmental Toxicology and Pharmacology, 2005, 19（1）：153-160.
[16] Lu LP, Xu LH, Kang TF, Cheng SY. DNA damage due to perfluorooctane sulfonate based on nanogold embedded in nanoporous poly pyrrole film[J]. Applied Surface Science, 2013, 284：258-262.
[17] CS, Yu K, Shi XJ, et al. Induction of oxidative stress and apoptosis by PFOS and PFOA in primary cultured hepatocytes of freshwater tilapia（Oreochromis niloticus）[J]. Aquatic Toxicology, 2007, 82（2）：135-143.
[18] T, Shabalina IG, Grander D, et al. Reactive oxygen species and mitochondria mediate the induction of apoptosis in human hepatoma HepG2 Cells by the rodent peroxisome proliferator and hepatocarcinogen, perfluorooctanoic acid[J]. Toxicology and Applied Pharmacology, 2001, 173（1）：56-64.
[19] DM, Li CD, Chen H, Shao B. Cellular response of freshwater green algae to perfluorooctanoic acid toxicity[J]. Ecotoxicology and Environmental Safety, 2013, 88：103-107.
[20] MA, Pacheco M, Ahmad I, Anguilla L. Antioxidants responses to in situ bleached kraft pulpmill effluent outlet exposure[J]. Environment International, 2004, 30（3）：301-308.
[21] AV, Søfteland L, Torstensen B, Olsvik PA. Transcriptional effects of PFOS in isolated hepatocytes from Atlantic salmon Salmo salar L[J]. Comparative Biochemistry and Physiology Part C：Toxicology & Pharmacology, 2008, 148（1）：14-22.
[22] KX, Chen GW, Lou H, F EI LN. Cloning and expression analysis of hsp70 gene from freshwater planarian Dugesia japonica[J]. Biologia, 2009.64（5）：1018-1024.