马铃薯AGPase活力反馈调控光合速率定量分析

生物技术通报 ›› 2014, Vol. 0 ›› Issue (11): 125-129.

马铃薯AGPase活力反馈调控光合速率定量分析

白桦¹,崔雪琼²,白少星³,姚新灵^3,

1.宁夏农业学校,银川 750021；2.郑州第二人民医院,郑州 450063；3.宁夏大学生命科学学院,银川 750021

收稿日期:2014-04-09 出版日期:2014-11-07 发布日期:2014-11-07
作者简介:白桦,女,副教授,研究方向：植物生理学
基金资助:
国家自然科学基金项目（30660079）,2012年宁夏科技支撑计划

Quantitative Analysis on Feedback of AGPase to Photosynetic Rate in Potato

Bai Hua¹,Cui Xueqiong²,Bai Shaoxing³,Yao Xinling³

1. Ningxia Agriculture School,Yinchuan 750021；2. Zhengzhou 2^nd Renmin Hospital,Zhengzhou 450063；3. Life Science School,Ningxia University,Yinchuan 750021

Received:2014-04-09 Published:2014-11-07 Online:2014-11-07

摘要/Abstract

摘要： 为了揭示ADP-葡萄糖焦磷酸化酶（AGPase）改变对叶片光合作用的反馈调控,以改变AGPase活力的马铃薯转化株系为材料,测定了其AGPase活力（AA）、块茎淀粉积累量（SC）、叶片光合速率（PR）、叶绿素（CC）和蔗糖含量（SUC）等；测定结果分析显示,各株系的AGPase活力（AA）、块茎淀粉积累量（SC）和叶片光合速率（PR）间存在显著差异,且均显著高于对照；株系间AGPase活力、块茎淀粉含量和叶片光合速率呈显著正相关性；每单位AGPase活力上升可贡献贮藏器官淀粉积累增加2.5%,且是影响叶片光合速率的重要因素；结果表明,AGPase不仅是下游淀粉积累的限速酶,而且可向上游反馈调控光合速率。

关键词: 淀粉含量, 光合速率, ADP-葡萄糖焦磷酸化酶, 相关性

Abstract: To reveal feedback regulation of ADP-pyrophosphrolase（AGPase）to photosynthesis, AGPase activities（AA）, starch contents（SC）in tuber, photosynthetic rates（PR）, chlorophyll contents（CC）and sucrose contents（SUC）in leafs were assayed with potato transgenic lines showing difference on ADP-pyrophosphrolase activities. The data analysis showed that AA, SC and PR in transgenic lines were significantly higher than control line. Significantly differences were found between AA, SC and PR among the lines. Correlation analysis showed that there were positive correlations between AA, SC and PR. Per unit rising of AGPase activities can contributed 2.5% increase of starch contents in sink tissue. AGPase was a factor playing a role in regulation of photosynthetic rates. The result indicated that AGPase not only regulates starch accomulation on the downstream, also feedback to modulate photosynthetic rate.

Key words: Starch contents, Photosynthetic rate , ADP-pyrophosphrolase, Correlation

白桦,崔雪琼,白少星,姚新灵. 马铃薯AGPase活力反馈调控光合速率定量分析[J]. 生物技术通报, 2014, 0(11): 125-129.

Bai Hua,Cui Xueqiong,Bai Shaoxing,Yao Xinling. Quantitative Analysis on Feedback of AGPase to Photosynetic Rate in Potato[J]. Biotechnology Bulletin, 2014, 0(11): 125-129.

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