细菌异源表达抗菌肽的研究进展

摘要/Abstract

摘要： 抗菌肽是一类从动植物、微生物体内分离得到的阳离子小分子量肽，具有天然的抗菌活性。它作用迅速，广谱，不易产生耐药性，具有重要的应用价值，近年来成为研究热点。普遍认为异源表达是生产大量抗菌肽的最有效方法。大肠杆菌作为经典的表达宿主，具有生长速度快、遗传背景清晰、有大量可利用的商业表达载体、易操作等优势，现已成为抗菌肽表达的首选宿主。乳酸菌作为世界公认安全的食品级微生物，近年来广泛用于抗菌肽的异源表达。着重阐述了抗菌肽在大肠杆菌、乳酸菌中重组表达的研究进展。

关键词: 抗菌肽, 异源表达, 表达系统, 大肠杆菌, 乳酸菌

Abstract: Antimicrobial peptides(AMPs)are a kind of cationic peptides which consist of short amino acid sequences and perform the defense system toward bacteria, virus. Different kinds of AMPs have been isolated from animals, plants, and microorganism. AMPs have important clinical application value due to their typically broad-spectrum activity against multiple pathogens. Heterogeneous expression has been demonstrated to be the most suitable tools to product antimicrobial peptides. Escherichia coli has a lot of advantages as a classic expression host, including its fast growth, clear genetic background, with a lot of available commercial expression vectors, easy operation and so on. E. coli become the preferred antimicrobial peptide expression host. Lactic acid bacteria have the advantage of being GRAS(generally recognized as safe), and have emerged in recent years as one of the most important heterogeneous expression strains of antimicrobial peptides. Developments of heterologous production of antimicrobial peptides in Escherichia coli and lactic acid bacteria were reviewed.

Key words: Antimicrobial, Peptide Heterogeneous expression, Expression System Escherichia, Coli Lactic acid bacteria

吕晓萌, 胡彤, 俞婷, 崔艳华. 细菌异源表达抗菌肽的研究进展[J]. 生物技术通报, 2014, 0(5): 37-44.

Lü Xiaomeng, Hu Tong, Yu Ting, Cui Yanhua. Advances of Heterogeneous Expression of Antimicrobial Peptides in Bacteria[J]. Biotechnology Bulletin, 2014, 0(5): 37-44.

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