玉米ZmCI-1B启动子及其7个5'端缺失体在转基因拟南芥中的活性分析

生物技术通报 ›› 2014, Vol. 0 ›› Issue (5): 69-75.

玉米ZmCI-1B启动子及其7个5'端缺失体在转基因拟南芥中的活性分析

李业^1，2 柳小庆² 李素贞³ 周晓今² 杨文竹² 陈茹梅²

(1.西南科技大学生命科学与工程学院，绵阳 621010；2.中国农业科学院生物技术研究所，北京 100081；3.河北农业大学农学院，保定 071001)

收稿日期:2014-02-20 出版日期:2014-05-23 发布日期:2014-05-24
作者简介:李业，女，硕士研究生，研究方向：植物学；E-mail：liye20086@163.com
基金资助:
转基因生物新品种培育科技重大专项(2013ZX08003-002)

Identification of ZmCI-1B Promoter and Its Seven Deletions in Transgenic Arabidopsis thaliana

Li Ye^1，2 Liu Xiaoqing² Li Suzhen³ Zhou Xiaojin² Yang Wenzhu² Chen Rumei²

(1. School of Life Science and Engineering，Southwest University of Science and Technology，Mianyang 621010；2. Department of Crop Genomics & Genetic Improvement，Biotechnology Research Institute，Chinese Academy of Agricultural Sciences，Beijing 100081；3. Department of Agronomy，Agricultural University of Hebei，Baoding 071001)

Received:2014-02-20 Published:2014-05-23 Online:2014-05-24

摘要/Abstract

摘要： 将ZmCI-1B全长启动子及其7个5'端缺失的启动子片段的表达载体分别转化农杆菌GV3101，经PCR鉴定正确之后，以拟南芥为遗传转化受体，利用花序侵染法将各个表达载体转化拟南芥。用PCR法检测转化后的拟南芥植株，取阳性植株的幼苗或花、角果进行GUS组织化学染色。结果表明，ZmCI-1B启动子在拟南芥中的调控特性与玉米中的不同；ZmCI-1B启动子及其7个5'端缺失启动子转基因拟南芥植株中GUS的染色部位各异，说明了不同长度的启动子功能特性不同，推测其可能与启动子上的顺式作用元件有关。

关键词: 启动子, 5&apos, 端缺失片段, 拟南芥, GUS染色

Abstract: The expression vectors of ZmCI-1B promoter and its seven 5' truncated fragments were transformed into Agrobacterium tumefaciens strain GV3101. After identified by PCR assay, with Arabidopsis thaliana as genetic transformation, the expression vectors were transformed into Arabidopsis thaliana by floral-dip method. The transformed Arabidopsis thaliana plants were identified by PCR assay, then the seedings, flowers and siliques from positive plants were conducted to GUS histochemical staining. The results showed that the characterization of ZmCI-1B promoter in Arabidopsis thaliana is different from miaze. The ZmCI-1B promoter and its seven 5' truncated promoter-GUS constructs had different GUS staining in transformed Arabidopsis thaliana plants, revealing that different-length promoter had different promoting activity. The cis-acting elements on the promoter may contribute to this.

Key words: Promoter 5&apos, truncated fragments, Arabidopsis thaliana, GUS, Staining

李业, 柳小庆, 李素贞, 周晓今, 杨文竹, 陈茹梅. 玉米ZmCI-1B启动子及其7个5'端缺失体在转基因拟南芥中的活性分析[J]. 生物技术通报, 2014, 0(5): 69-75.

Li Ye, Liu Xiaoqing, Li Suzhen, Zhou Xiaojin, Yang Wenzhu, Chen Rumei. Identification of ZmCI-1B Promoter and Its Seven Deletions in Transgenic Arabidopsis thaliana[J]. Biotechnology Bulletin, 2014, 0(5): 69-75.

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