小麦新抗源CH5383抗条锈病基因的遗传分析及分子定位

生物技术通报 ›› 2014, Vol. 0 ›› Issue (6): 96-100.

小麦新抗源CH5383抗条锈病基因的遗传分析及分子定位

詹海仙^1，2, 畅志坚², 李光蓉¹, 贾举庆³, 郭慧娟², 张晓军², 李欣², 乔麟轶², 杨足君¹

（1.电子科技大学生命科学与技术学院，成都 610054；2.山西省农业科学院作物科学研究所，太原 030031； 3.山西农业大学农学院，太谷 030801）

收稿日期:2013-11-06 出版日期:2014-06-25 发布日期:2014-06-25
作者简介:詹海仙，女，博士研究生，研究方向：小麦分子生物学；E-mail：zhan030006@126.com
基金资助:
国家自然科学基金项目（31171839），山西省留学基金项目（2012-102），山西省国际合作项目（2012081006-2）

Genetic Analysis and Molecular Mapping of Stripe Rust Resistance Gene in Wheat Line CH5383

Zhan Haixian^1，2,Chang Zhijian²,Li Guangrong¹,Jia Juqing³,Guo Huijuan²,Zhang Xiaojun²,Li Xin²,Qiao Linyi²,Yang Zujun¹

（1. School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054； 2. Institute of Crop Science, Shanxi Academy of Agricultural Sciences, Taiyuan 030031； 3. College of Agronomy, Shanxi Agricultural University, Taigu 030801）

Received:2013-11-06 Published:2014-06-25 Online:2014-06-25

摘要/Abstract

摘要： CH5383是新育成的源于中间偃麦草的渗入系，对小麦条锈病和白粉病均表现免疫。为明确其抗性来源、遗传方式和抗病基因在染色体上的位置，将CH5383的系谱材料及其与高感条锈病品种（系）杂交的F₁、F₂和F_2：3家系群体进行条锈病抗性鉴定。结果表明，CH5383对条锈病的抗性源于中间偃麦草，对条锈病生理小种CYR32的抗性由一对显性核基因控制，将此基因暂时命名为YrCH5383。从476对SSR引物中筛选到3对引物Xgwm108、Xbarc206和Xbarc77与抗病基因连锁，遗传距离分别是8.2 cM、10.7 cM和13.6 cM。根据这两对标记在染色体上的位置，将抗病基因定位到3B染色体的长臂上。3B染色体的长臂还未见有正式命名的抗条锈病基因的报道，推测YrCH5383可能是一个源于中间偃麦草的新抗条锈病基因。

关键词: 小麦, 条锈病, 中间偃麦草, 遗传分析, 分子定位

Abstract: CH5383 is an introgression lines from Thinopyrum intermedium with high resistance to wheat stripe rust and powdery mildew. The evaluations of disease were tested using Pst races CYR31 and CYR32 in seedlings. The result showed that the stripe rust resistance came from Thinopyrum intermedium in CH5383. Genetic analysis revealed that the resistance was controlled by a single dominant gene inoculating Pst race CYR32 inadult stage. It was temporarily named YrCH5383. Two polymorphic SSR markers, Xgwm108, Xbarc206 and Xbarc77 were linked to the resistance gene with genetic distance 8.2 cM, 10.7 cM and 13.6 cM, respectively. Based on marker loci and the origination, YrCH5383 might be a new gene to wheat stripe rust on 3BL.

Key words: Wheat, Stripe rust, Thinopyrum intermedium, Genetic analysis, Molecular mapping

詹海仙, 畅志坚, 李光蓉, 贾举庆, 郭慧娟, 张晓军, 李欣, 乔麟轶, 杨足君. 小麦新抗源CH5383抗条锈病基因的遗传分析及分子定位[J]. 生物技术通报, 2014, 0(6): 96-100.

Zhan Haixian,Chang Zhijian,Li Guangrong,Jia Juqing,Guo Huijuan,Zhang Xiaojun,Li Xin,Qiao Linyi,Yang Zujun. Genetic Analysis and Molecular Mapping of Stripe Rust Resistance Gene in Wheat Line CH5383[J]. Biotechnology Bulletin, 2014, 0(6): 96-100.

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