质谱非标记定量分析小麦抗条锈病近等基因系 蛋白质变化

生物技术通报 ›› 2014, Vol. 0 ›› Issue (6): 88-95.

质谱非标记定量分析小麦抗条锈病近等基因系蛋白质变化

黄宇^1，2，3, 冯晶⁴, 饶力群¹, 徐世昌⁴, 潘映红^2，3

（1.湖南农业大学生物科学技术学院，长沙 410128；2.中国农业科学院作物科学研究所，北京 100081；3.国家农作物基因资源与基因改良重大科学工程，北京 100081；4.中国农业科学院植物保护研究所，北京 100193）

收稿日期:2013-12-16 出版日期:2014-06-25 发布日期:2014-06-25
作者简介:黄宇，男，硕士研究生，研究方向：生物化学与分子生物学；E-mail：nictkk@163.com
基金资助:
国家“863”高技术研究发展计划（2008AA10Z115），国家自然科学基金项目（30971874），转基因生物新品种培育科技重大专项（2009ZX08012-011B）

Label-free Quantitative Proteomics Analysis of a Wheat Near-isogenic Line Pair with Q Exactive Mass Spectrometer

Huang Yu^1，2，3,Feng Jing⁴,Rao Liqun¹,Xu Shichang⁴,Pan Yinghong^2，3

（1. College of Bbioscience and Biotechnology，Hunan Agriculture University，Changsha 410128；2. Institute of Crop Science，Chinese Academy of Agricultural Sciences，Beijing 100081；3. The National Key Facility for Crop Gene Resources and Genetic Improvement，Beijing 100081；4. Institute of Plant Protection，Chinese Academy of Agricultural Sciences，Beijing 100193）

Received:2013-12-16 Published:2014-06-25 Online:2014-06-25

摘要/Abstract

摘要： 利用基于液相色谱串联Orbitrap质谱（Q Exactive）的非标定量（Label-free）技术，对小麦抗病单基因近等基因系Taichung29*6/Yr10和背景品种Taichung29叶片的蛋白质表达进行了比较分析。经MASCOT软件搜库，在两个样品中共同鉴定到2 257个蛋白，其中有准确定量信息的蛋白共1 549个，含量变化大于2倍的差异蛋白有102个。对102个差异表达蛋白进行了Gene Ontology（GO）功能注释和分析，发现他们多数定位于细胞基质和核糖体等细胞器，具备结合、催化等功能，主要参与代谢、细胞过程、应激等生物学进程，其中超氧化物歧化酶、甲硫氨酰氨肽酶、溶酶体-β-葡萄糖苷酶和铁蛋白等可能与小麦抗病单基因近等基因系Taichung 29*6/Yr10的抗病性有一定相关性。

关键词: 小麦, 条锈病, 抗病性, 蛋白质组学, 非标记定量 Q, Exactive质谱

Abstract: In this study, the protein expression of a wheat near-isogenic line pair, Taichung 29*6/Yr10 and Taichung 29, have been compared with label-free quantitative proteomic approach based on liquid chromatography tandem Q Exactive hybrid quadrupole-Orbitrap mass spectrometer. By MASCOT database search, a total of 2 257 proteins were identified from both of the samples, and among of them 1 549 proteins were accurately quantitated and 102 differentially expressed proteins（>2 fold）were detected. Gene ontology（GO）analysis showed that these differential proteins were mainly localized to cell matrix and organelles such as ribosome, and mainly involved in binding and catalytic functions. Among of 102 proteins which mainly take part in metabolic, cellular and stress responses processes, Superoxide dismutase, Methionine aminopeptidase, Lysosomal-β-glucosidase, and Ferritin may play some important roles in Taichung 29*6/Yr10 resistance to Yellow rust.

Key words: Wheat, Yellow rust, Resistance, Proteomics Label-free quantitation, Q, Exactive, Mass, Spectrometer

黄宇, 冯晶, 饶力群, 徐世昌, 潘映红. 质谱非标记定量分析小麦抗条锈病近等基因系蛋白质变化[J]. 生物技术通报, 2014, 0(6): 88-95.

Huang Yu,Feng Jing,Rao Liqun,Xu Shichang,Pan Yinghong. Label-free Quantitative Proteomics Analysis of a Wheat Near-isogenic Line Pair with Q Exactive Mass Spectrometer[J]. Biotechnology Bulletin, 2014, 0(6): 88-95.

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