柴油降解菌Acinetobacter sp. AK5的筛选及其降解性能研究

摘要/Abstract

摘要：

从污水处理厂的活性污泥中分离到一株柴油降解菌，通过生理生化鉴定和16S rDNA序列分析，鉴定该菌为不动杆菌 Acinetobacter sp. AK5。检测了不同pH值、NaCl浓度、培养时间和各种柴油浓度下Acinertobacter sp. AK5的柴油降解情况。结果表明，该菌的最适生长初始pH值为5-9，适合NaCl浓度为3%-4%，柴油浓度为5 g/L时，该菌7 d柴油降解率可达99%，柴油浓度为20 g/L时，7 d柴油降解率也可达67%。AK5在人工海水培养基中及无机盐培养基中生长状态良好，在海水和淡水石油污染的生物修复中具有很好的应用前景。

关键词: 柴油, 降解, 不动杆菌, 降解率

Abstract:

A diesel degradable bacterial strain was isolated from activated sludge and identified as Acinetobacter sp. AK5 through physiological, biochemical identification and 16S rDNA sequence analysis. Experiments of the different pH values, NaCl concentrations, culture time and diesel concentrations were detected to evaluate the diesel degradability by Acinetobacter sp. AK5. The results show that the optimal initial pH scope for the bacterial growth is from 5 to 9, the optimum NaCl concentrations is between 3% and 4%. When the diesel concentration is 5 g/L, the 7 d diesel degradation rate can reach 99%, while when the concentration of diesel is 20 g/L, 7 d diesel degradation rate can be 67%. The Acinetobacter sp. AK5 can grow well in artificial seawater medium and inorganic salt culture medium, therefore it has promising application prospect in seawater and freshwater oil pollution treatment.

Key words: Diesel, Degradation, Acinetobacter, Degradation rate

徐晓宇, 陈敬华. 柴油降解菌Acinetobacter sp. AK5的筛选及其降解性能研究[J]. 生物技术通报, 2014, 0(8): 146-151.

Xu Xiaoyu, Chen Jinghua. Isolation of Diesel Degrading Strain Acinetobacter sp. AK5 and Its Degrading Performance[J]. Biotechnology Bulletin, 2014, 0(8): 146-151.

参考文献

［1］周长征, 李秀云, 宋延博.三维荧光法在石油污染鉴别中的应用［J］.光谱学与光谱分析, 1998, 18（4）：500-502.
［2］Watanabe K, Kodama Y, Syutsubo K. Molecular characterization of bacterial populations in petroleum-contaminated ground water discharged from underground crude oil storage cavities［J］. Appl Environ. Microbiol, 2000, 66（11）：4803-4809.
［3］景伟文, 杨桂朋, 康志强.海洋溢油污染对生物群落和种群的影响及生态系统的恢复［J］.海洋湖沼通报, 2008（1）：81-88.
［4］Mohanty G, Mukherji S. Biodegradation rate of diesel range n-alkanes by bacterial cultures Exiguobacterium aurantiacum and Burkholderia cepacia Gita Mohanty, Suparna Mukherji［J］. International Biodeterioration & Biodegradation, 2008, 61：240-250.
［5］Mercedes M, Ana P, Santiago R, et al. Biodegradation of diesel and heating oil by Acinetobacter calcoaceticus MM5：its possible applications on bioremediation［J］. International Biodeterioration & Biodegradation, 1995, 35：269-285.
［6］Nievas ML, Commendatore MG, Esteves JL, Bucal V. Biodegradation pattern of hydrocarbons from a fuel oil-type complex residue by an emulsifier-producing microbial consortium［J］. Journal of Hazardous Materials, 2008, 154：96-104.
［7］Wang LM, Liu PW, Ma CC, Cheng SS. Application of biosurfactants, rhamnolipid, and surfactin, for enhanced biodegradation of diesel-contaminated water and soil［J］. Journal of Hazardous Materials, 2008, 151：155-163.
［8］Wang LM, Liu PWG, Ma CC, Cheng SS. Application of rhamnolipid and surfactin for enhanced diesel biodegradation—Effects of pH and ammonium addition［J］. Journal of Hazardous Materials, 2009, 164：1045-1050.
［9］Wyrwas B, Chrzanowski ?, ?awniczak ?, et al. Utilization of Triton X-100 and polyethylene glycols during surfactant-mediated biodegradation of diesel fuel［J］. Journal of Hazardous Materials, 2011, 197：97-103.
［10］stberg TL, Jonsson AP, Bylund D, Lundstr?m US. The effects of carbon sources and micronutrients in fermented whey on the biodegradation of n-hexadecane in diesel fuel contaminated soil［J］. International Biodeterioration & Biodegradation, 2007, 60（4）：334-341.
［11］Jonsson A, ?stberg TL. The effects of carbon sources and micronu-trients in whey and fermented whey on the kinetics of phenanthrene biodegradation in diesel contaminated soil［J］. Journal of Hazardous Materials, 2011, 192（3）：1171-1177.
［12］Manuela T, Vesna M, Francesca C, et al. Effects of biostimulation and bioaugmentation on diesel removal and bacterial community［J］. International Biodeterioration & Biodegradation, 2012, 66：39-46.
［13］Bregnard T, Hohener PA, Zeyer FP. Bioavailability and biodegradation of weathered diesel fuel in aquifer material under denitrifying conditions［J］. Environ Toxicol Chem, 1998, 17：1222-1229.
［14］Aeckersberg F, Bak F, Widdel F. Anaerobic oxidation of saturated hydrocarbons to CO₂ by a new type of sulfate-reducing bacterium［J］. Arch Mirobiol, 1991, 156：5-14.
［15］Rueter P, Rabus R, Wilkes H, et al. Anaerobic oxidation of hydrocarbons in crude oil by new types of sulphate-reducing bacteria［J］. Nature, 1994, 372：455-458.
［16］Kleikemper J, Schroth MH, Sigler W, et al. Activity and diversity of sulfate-reducing bacteria in a petroleum hydrocarbon-contaminated aquifer［J］. Appl Environ Microbiol, 2002, 68：1516-1523.
［17］王丽萍, 刘昱慧, 邵宗泽.一株来自大西洋表层海水的烷烃降解菌Gordonia sp. S14-10的分离鉴定及其降解相关特性的分析［J］.微生物学报, 2009, 12：1634-1642.
［18］吴业辉, 邵宗泽.海洋烷烃降解菌Alcanivorax sp. A-11-3的分离鉴定及其降解酶基因研究［J］.台湾海峡, 2008（5）：427-434.
［19］武金装, 刘红玉, 曾光明, 等.柴油降解菌的筛选及其降解特性研究［J］.农业环境科学学报, 2008, 27（5）：1742-1746.
［20］谢重阁.环境中石油污染物的分析技术［M］.北京：中国环境科学出版社, 1987：82-83.
［21］刘陈立, 邵宗泽.海洋石油降解微生物的分离鉴定［J］.海洋学报, 2005（7）：114-119.
［22］陈碧娥, 刘祖同.湄洲湾海洋细菌降解石油烃研究［J］.石油学报（石油加工）, 2001, 17（5）：31-35.
［23］谭田丰, 邵宗泽.海洋石油烃降解菌群构建及其在降解过程中的动态分析［J］.厦门大学学报：自然科学版, 2006, 45：262-266.
［24］苏莹, 陈莉, 汪辉, 刘兆普.海洋石油降解菌的筛选与降解特性［J］.应用与环境生物学报, 2008, 14（4）：518-522.
［25］周常义, 林情员, 苏国成, 等.一株海洋石油烃降解菌的分离鉴定及特性研究［J］.集美大学学报：自然科学版, 2009, 1：24-28.

[1]	娄慧, 朱金成, 杨洋, 张薇. 抗、感品种棉花根系分泌物对尖孢镰刀菌生长及基因表达的影响[J]. 生物技术通报, 2023, 39(9): 156-167.
[2]	吴巧茵, 施友志, 李林林, 彭政, 谭再钰, 刘利平, 张娟, 潘勇. 类胡萝卜素降解菌株的原位筛选及其在雪茄提质增香中的应用[J]. 生物技术通报, 2023, 39(9): 192-201.
[3]	江海溶, 崔若琪, 王玥, 白淼, 张明露, 任连海. NH₃和H₂S降解功能菌的分离鉴定及降解特性研究[J]. 生物技术通报, 2023, 39(9): 246-254.
[4]	饶紫环, 谢志雄. 一株Olivibacter jilunii 纤维素降解菌株的分离鉴定与降解能力分析[J]. 生物技术通报, 2023, 39(8): 283-290.
[5]	潘虎, 周子琼, 田云. 三株异菌脲高效降解菌株的筛选、鉴定及其降解特性分析[J]. 生物技术通报, 2023, 39(6): 298-307.
[6]	王羽, 尹铭绅, 尹晓燕, 奚家勤, 杨建伟, 牛秋红. 烟草甲体内烟碱降解菌的筛选、鉴定及降解特性[J]. 生物技术通报, 2023, 39(6): 308-315.
[7]	张晶, 张浩睿, 曹云, 黄红英, 曲萍, 张志萍. 嗜热纤维素降解菌研究进展[J]. 生物技术通报, 2023, 39(6): 73-87.
[8]	马玉倩, 孙东辉, 岳浩峰, 辛佳瑜, 刘宁, 曹志艳. 具有辅助降解纤维素功能的大斑刚毛座腔菌糖苷水解酶GH61的鉴定、异源表达及功能分析[J]. 生物技术通报, 2023, 39(4): 124-135.
[9]	杨冬, 唐璎. 枯草芽孢杆菌WTX1胞外酶降解AFB₁酶学特性及降解位点分析[J]. 生物技术通报, 2023, 39(4): 93-102.
[10]	张傲洁, 李青云, 宋文红, 颜少慧, 唐爱星, 刘幽燕. 基于苯酚降解的粪产碱杆菌Alcaligenes faecalis JF101的全基因组分析[J]. 生物技术通报, 2023, 39(10): 292-303.
[11]	高晓蓉, 丁尧, 吕军. 芘降解菌Pseudomonas sp. PR3的植物促生特性及其对芘胁迫下水稻生长的影响[J]. 生物技术通报, 2022, 38(9): 226-236.
[12]	李柳, 穆迎春, 刘璐, 张洪玉, 徐锦华, 杨臻, 乔璐, 宋金龙. 氟喹诺酮类抗生素及耐药基因污染控制的研究进展[J]. 生物技术通报, 2022, 38(9): 84-95.
[13]	刘广超, 叶青, 车永梅, 李雅华, 安东, 刘新. 烟草根际高效解磷菌的筛选鉴定及促生作用研究[J]. 生物技术通报, 2022, 38(8): 179-187.
[14]	王亚军, 司运美. 除磷菌CP-7的筛选及其降解特性研究[J]. 生物技术通报, 2022, 38(7): 258-268.
[15]	王新光, 田磊, 王恩泽, 钟成, 田春杰. 玉米秸秆高效降解微生物复合菌系的构建及降解效果评价[J]. 生物技术通报, 2022, 38(4): 217-229.