陶厄氏菌Thauera sp. K11对酚类化合物降解作用及途径研究

doi:10.13560/j.cnki.biotech.bull.1985.2017-0452

摘要/Abstract

摘要： 旨在分析陶厄氏菌属（Genus Thuaera）中的一株菌株Thauera sp. K11对含酚废水中酚类化合物的降解作用和途径。以石化污水厂分离菌株K11为研究对象，克隆其16S rRNA基因和关键酶基因，并进行系统发育分析，在基因水平探究苯酚降解机理；利用气相色谱技术检测酚类化合物降解效果和苯酚降解机理。结果显示，利用16S rRNA系统学分析发现K11是陶厄氏菌属的一株细菌。该菌对11种酚类化合物具有降解作用，其中5种酚类化合物72 h的降解率>90%。克隆并获得了K11的苯酚羟化酶和邻苯二酚双加氧酶基因。酶活性测定表明，K11通过苯酚羟化酶催化苯酚转化为邻苯二酚，然后利用邻苯二酚-2，3-双加氧酶催化产生2-HMSA。陶厄氏菌Thauera sp. K11是一株能够降解多种酚类化合物的菌株，具有较强的酚类污染物降解能力，其通过苯酚→邻苯二酚→2-HMSA途径进行苯酚降解。

关键词: 陶厄氏菌属, 石化废水, 酚类化合物, 生物降解, 苯酚羟化酶, 邻苯二酚双加氧酶

Abstract: The objective of this work is to analyze the degradation and pathways of a Thauera sp. K11 in genus Thauera to phenolic compounds in phenolic wastewater. Thauera sp. K11 was isolated from petrochemical wastewater treatment plant .The 16S rRNA gene and key enzyme gene were cloned and analyzed and phylogenetic analysis was carried out. The mechanism of phenol degradation was explored at the gene level. The degradation efficiency and mechanism of phenolic compounds were studied by gas chromatography. The 16S rRNA phylogenetic analysis revealed that the bacterium Thauera sp. K11 was a species of bacteria from the genus Thauera，which degraded 11 phenolic compounds. The degradation rates of the five phenolic compounds were > 90 % in 72 h. The K11 phenol hydroxylase and catechol dioxygenase genes were cloned，and the enzyme activity assay showed that K11 catalyzed the conversion of phenol to catechol by phenol hydroxylase and then catalyzed the production of 2-HMSA with catechol-2，3-dioxygenase. In conclusion，the bacterium Thauera sp. K11 is a species of Thauera bacterium capable of degrading a variety of phenolic compounds，and possesses the strong capability of degrading phenolic pollutants；the degradation pathway is by phenol→catechol→2-HMSA.

Key words: genus Thauera, petrochemical wastewater, phenolic compounds, biodegradation, phenol hydroxylase, catechol-2, 3-dioxygenase

乔能虎, 张静静, 刘德健, 汤孝胜, 郗丽君, 刘建国. 陶厄氏菌Thauera sp. K11对酚类化合物降解作用及途径研究[J]. 生物技术通报, 2017, 33(10): 184-190.

QIAO Neng-hu, ZHANG Jing-jing, LIU De-jian, TANG Xiao-sheng, XI Li-jun, LIU Jian-guo. Study On the Degradation and Mechanism of Phenolic Compounds by the Thauera sp. K11[J]. Biotechnology Bulletin, 2017, 33(10): 184-190.

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