优化大肠杆菌表达外源蛋白的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2017.02.003

摘要/Abstract

摘要： 大肠杆菌表达系统是目前表达外源蛋白的首选,利用该表达系统表达重组蛋白有许多优越性,但表达的外源蛋白容易被宿主蛋白酶攻击或未能正确折叠形成包涵体,其表达受到了限制。综述了国内外利用大肠杆菌表达外源蛋白过程中采用的一些优化策略,主要包括表达稀有密码子、应用融合标签,改变表达菌株、降低包涵体形成、单一蛋白技术、自诱导、流加培养以及高密度细胞培养等,以期探索外源蛋白表达的最优策略。

关键词: 大肠杆菌, 外源蛋白, 流加培养, 高密度培养

Abstract: Escherichia coli expression system has become the first choice of expression of exogenous protein,the expression system for recombinant protein presents many advantages,and however,sometimes expressed exogenous protein is easily attacked by host protease or cannot form insoluble inclusion bodies because of incorrect folding,thus by which the expression is limited. This paper summarizes some optimization strategies used in the process of the expression of exogenous proteins in E. coli,mainly including the expression of rare codons,application of fusion tags,change of E. coli strains for expression,and reducing the formation of inclusion bodies,a single protein production system,auto-induction,fed-batch cultivation,high cell-density culture,etc.,which aims at exploring the optimal strategy for the expression of exogenous protein.

Key words: Escherichia coli, exogenous protein, fed-batch cultivation, high-cell-density cultivation

苏鹏, 龚国利. 优化大肠杆菌表达外源蛋白的研究进展[J]. 生物技术通报, 2017, 33(2): 16-23.

SU Peng, GONG Guo-li. Research Progress on Optimizing the Expression of Exogenous Proteins in Escherichia coli[J]. Biotechnology Bulletin, 2017, 33(2): 16-23.

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