一株解淀粉芽孢杆菌生防蛋白的鉴定及分析

doi:10.13560/j.cnki.biotech.bull.1985.2017-0630

生物技术通报 ›› 2018, Vol. 34 ›› Issue (1): 202-207.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0630

一株解淀粉芽孢杆菌生防蛋白的鉴定及分析

郭继平¹, 马光¹, 王志杰¹, 齐善厚¹, 王宝梅³, 苏长青^1,2

1. 衡水学院生命科学系,衡水 053000;
2. 中国农业科学院植物保护研究所,北京 100193;
3. 宝鸡市农业科学院,宝鸡 722400

收稿日期:2017-07-28 出版日期:2018-01-26 发布日期:2018-01-22
作者简介:郭继平,女,博士,副教授,研究方向：植物病害生物防治;E-mail：guojiping888@163.com
基金资助:
河北省青年拔尖人才支持计划项目（201619）,河北省高等学校青年拔尖人才研究计划项目（BJ201608）

Identification and Analysis of Biocontrol Proteins from a Strain of Bacillus amyloliquefaciens

GUO Ji-ping¹, MA Guang¹, WANG Zhi-jie¹, QI Shan-hou¹, WANG Bao-mei³, SU Chang-qing^1,2

1. Department of Life Science,Hengshui University,Hengshui 053000;
2. Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193;
3. Baoji Academy of Agricultural Sciences,Baoji 722400

Received:2017-07-28 Published:2018-01-26 Online:2018-01-22

摘要/Abstract

摘要： 旨在鉴定一株拮抗葡萄霜霉病的解淀粉芽孢杆菌在NA液体培养基中的分泌蛋白中与生物防治相关的蛋白组分。采用蛋白质谱的方法对前期筛选到的一株拮抗葡萄霜霉病的解淀粉芽孢杆菌生防菌的在NA液体培养基中的分泌蛋白进行了鉴定。对鉴定到的蛋白采用基因本体论（GO）和京都基因与基因组百科全书（KEGG）的方法进行蛋白质组学分析,筛选与生物防治相关的蛋白。结果表明,从分泌蛋白中鉴定到了确定的53种蛋白质,其相对分子量大部分集中到0-100之间（占79.24%）;涉及碳水化合物代谢、能量代谢、脂类代谢、氨基酸代谢和生物防御等过程,以及作为细胞组分参与结构组成。发现了6种参与植物与病原菌互作的蛋白,2种属于氨基肽酶家族,4种参与几丁质的生物降解过程。

关键词: 解淀粉芽孢杆菌, 生物防治, 基因本体论, 氨基肽酶, 几丁质酶

Abstract: It is aimed at the identification of protein composition with biocontrol function in the secretory protein while a strain of being antagonistic grape downy mildew,Bacillus amyloliquefaciens,was cultured in NA liquid medium,which was carried out by protein profile. The proteomics of the identified proteins was analyzed by gene ontology and KEGG pathway for screening the proteins related to biocontrol. The results showed that 53 proteins were identified from the secreted proteins. Their relative molecular weights were mostly（79.24%）in 0-100. The proteins involved in the processes of carbohydrate metabolism,energy metabolism,lipid metabolism,amino acid metabolism,bio-defense,etc. They also were the cellular components in structural composition. Six proteins involved in the interaction of plant and pathogen were discovered,and among them,two belonged to the aminopeptidase family,and four were involved in the biodegradation of chitin.

Key words: Bacillus amyloliquefaciens, biocontrol, gene ontology, aminopeptidase, chitinase

郭继平, 马光, 王志杰, 齐善厚, 王宝梅, 苏长青. 一株解淀粉芽孢杆菌生防蛋白的鉴定及分析[J]. 生物技术通报, 2018, 34(1): 202-207.

GUO Ji-ping, MA Guang, WANG Zhi-jie, QI Shan-hou, WANG Bao-mei, SU Chang-qing. Identification and Analysis of Biocontrol Proteins from a Strain of Bacillus amyloliquefaciens[J]. Biotechnology Bulletin, 2018, 34(1): 202-207.

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一株解淀粉芽孢杆菌生防蛋白的鉴定及分析

Identification and Analysis of Biocontrol Proteins from a Strain of Bacillus amyloliquefaciens

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