抗菌肽在洗发液中防腐效能及安全性评价初探

doi:10.13560/j.cnki.biotech.bull.1985.2018-0288

生物技术通报 ›› 2018, Vol. 34 ›› Issue (10): 217-224.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0288

抗菌肽在洗发液中防腐效能及安全性评价初探

桂银雪^{1, 4}, 杨娜^{3, 4}, 滕达^{2, 3, 4}, 王秀敏^{2, 3, 4}, 毛若雨^{2, 3, 4}, 郝娅^{2, 3, 4}, 滕国新¹, 王建华^{2, 3, 4}

1. 北京城市学院航天城校区生物医药学部,北京 100094;
2. 北京生泰云科技有限公司研发中心,北京 100081;
3. 农业农村部饲料生物技术重点开放实验室,北京 100081;
4. 中国农业科学院饲料研究所基因工程研究室,北京 100081

收稿日期:2018-03-29 出版日期:2018-10-26 发布日期:2018-11-07
作者简介:桂银雪,女,研究方向：食品质量与安全;E-mail：guiyinxue@163.com,杨娜同为本文第一作者
基金资助:
国家自然科学基金项目（31702146,31572445,31772640,31601968,31672456）,国家农业科技创新工程重大产出科研选题—饲用抗生素替代关键产品创制与产业化（CAAS-ZDXT2018008,2018-2020）,北京高等学校高水平人才交叉培养“实培计划”

Preliminary Study on Preservative Efficacy and Safety Evaluation of Antimicrobial Peptides in Shampoo

GUI Yin-xue^{1, 4}, YANG Na^{3, 4}, TENG Da^{2, 3, 4}, WANG Xiu-min^{2, 3, 4}, MAO Ruo-yu^{2, 3, 4}, HAO Ya^{2, 3, 4}, TENG Guo-xin¹, WANG Jian-hua^{2, 3, 4}

1. School of Biomedicine,Beijing City University,Beijing 100094;
2. D&R Center,Beijing Shengtai Clouds Bio-Technology Inc.,Beijing 100081;;
3. Key Laboratory of Feed Biotechnology,Ministry of Agriculture,Beijing 100081;
4. Gene Engineering Laboratory,Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081

Received:2018-03-29 Published:2018-10-26 Online:2018-11-07

摘要/Abstract

摘要： 防腐剂是化妆品中防止微生物污染的重要成分。为获得高效防腐且无毒副作用的洗发液防腐剂,实验选取4种抗菌肽进行MIC测定,筛选出针对个革兰氏阴性菌和阳性菌杀菌效果较好的两个抗菌肽N2和NZ2114。通过体外抗菌试验和细胞水平及小鼠皮肤刺激性实验对两种抗菌肽的防腐效果和安全性能进行测定和评估。实验结果表明,N2和NZ2114在低浓度（分别为40 和20 µg/mL）下即具有快速高效的杀菌效果,并且高浓度（128 µg/mL）下,细胞存活率大于85%,具有低细胞毒性。ROS水平和小鼠皮肤刺激性实验证明,N2和NZ2114不会对皮肤细胞造成损伤,并且具有一定程度的抗氧化作用。本实验结果为抗菌肽作为高效安全的洗发液防腐剂提供参考依据。

关键词: 防腐剂, 抗菌肽, 防腐效果, 安全性能

Abstract: Preservatives are important ingredients to prevent cosmetics from microbial contamination. In order to have effective but non-toxic preservatives,the MICs of four antimicrobial peptides were determined,and two peptides N2 and NZ2114 having favorable antibacterial effects towards Gram-negative and -positive bacteria were screened. The preservative effect and safety performance were measured and evaluated by antibacterial experiments in vitro,ROS level and skin irritation test in mice. The results showed that,N2 and NZ2114 had a fast and efficient sterilization effect at the low concentration scope（40 and 20 µg/mL respectively）,and the cell survival rate was more than 85% at a high concentration（128 µg/mL）,moreover,they had low cytotoxicity. The results of ROS level and skin irritation test in mice demonstrated that N2 and NZ2114 did not damage skin cells and had antioxidant effect to some extent. These results provide a reference for using antimicrobial peptides a potent candidate of new effective and safe preservative in shampoo.

Key words: preservative, antibacterial peptides, preservative effect, safety performance

桂银雪, 杨娜, 滕达, 王秀敏, 毛若雨, 郝娅, 滕国新, 王建华. 抗菌肽在洗发液中防腐效能及安全性评价初探[J]. 生物技术通报, 2018, 34(10): 217-224.

GUI Yin-xue, YANG Na, TENG Da, WANG Xiu-min, MAO Ruo-yu, HAO Ya, TENG Guo-xin, WANG Jian-hua. Preliminary Study on Preservative Efficacy and Safety Evaluation of Antimicrobial Peptides in Shampoo[J]. Biotechnology Bulletin, 2018, 34(10): 217-224.

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抗菌肽在洗发液中防腐效能及安全性评价初探

Preliminary Study on Preservative Efficacy and Safety Evaluation of Antimicrobial Peptides in Shampoo

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