植物内生细菌修复重金属污染土壤作用机制研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2018-0593

摘要/Abstract

摘要： 内生细菌生活在植物组织内部,长期以来与宿主植物形成了紧密的共生关系。内生细菌在重金属吸收、耐受和解毒方面具有优良的特性,为修复重金属污染土壤提供了有效的新方案。综述了内生细菌强化植物修复重金属污染土壤的作用机制,包括内生细菌通过产生植物生长调节激素,分泌ACC脱氨酶和几丁质酶等,促进宿主植物在重金属胁迫条件下的生长;通过改变重金属的生物有效性/毒性,减轻植物重金属毒害;通过与植物形成联合修复体,加强植物抗重金属毒性的能力。分析了近几年超富集植物内生细菌多样性及其影响因素,探讨了联合修复过程中影响内生细菌强化修复效果的主要因素,包括内生细菌的来源、活性和环境胁迫等各种生物因素和非生物因素,并对内生细菌与植物联合修复的研究方向进行展望,涉及内生细菌自身存活原因和如何耐受重金属的机制研究,植物内生细菌的行为动力学和代谢,以及内生细菌、植物及土壤之间的生态互作效应等,以期推动内生细菌大规模应用于植物修复重金属污染土壤。

关键词: 内生细菌, 重金属, 修复机制

Abstract: Endophytic bacteria reside within plant hosts and have a close symbiotic association with host plants. Their excellent properties in heavy metal absorption,tolerance and detoxification provide a novel approach for the remediation of contaminated soil by heavy metals. This review concentrates on the fundamental mechanism of endophyte-assisted phytoremediation of heavy metal-contaminated soils,including the promoting the growth of host plants under heavy metals by producing plant growth regulating hormone and secreting ACC deaminases and chitinase and so on,reducing the damages of heavy metals to plants by altering the bioavailability /or toxicity of heavy metal,and improving the resistance of plants to heavy metal stress by forming joint remediating complex. Then recent progress in characterizing the diversity of endophytic bacteria from hyperaccumulator and its influencing factors are also reviewed. Furthermore,the main factors influencing the effect of endophytic bacteria in the phytoremediation are discussed,including the source and activity of endophytic bacteria and various biotic and abiotic factors of environmental stress. Special attention is paid to the future study in the field of endophyte-assisted phytoremediation technology,such as the mechanism of endophytic bacteria’s own survival and how to tolerate heavy metals,behavioral dynamics and metabolism of endophytic bacteria,and the ecological interaction between endophytic bacteria,plants and soil will be considered,aiming at promoting the large-scale application of endophytic bacteria in the phytoremediation of heavy metal-contaminated soil.

Key words: endophytic bacteria, heavy metal, remediation mechanism

张萌萌, 曹立东, 王仁卿, 孙瑞莲. 植物内生细菌修复重金属污染土壤作用机制研究进展[J]. 生物技术通报, 2018, 34(11): 42-49.

ZHANG Meng-meng, CAO Li-dong, WANG Ren-qing, SUN Rui-lian. Mechanism of Endophytic Bacteria Remediating Heavy Metal-Contaminated Soil[J]. Biotechnology Bulletin, 2018, 34(11): 42-49.

参考文献

[1] Sheng XF, Xia JJ, Jiang CY, et al.Characterization of heavy metal-resistant endophytic bacteria from rape(Brassica napus)roots and their potential in promoting the growth and lead accumulation of rape[J]. Environmental Pollution, 2008, 156(3):1164-1170.
[2] Chen B, Zhang Y, Rafiq MT, et al.Improvement of cadmium uptake and accumulation in Sedum alfredii by endophytic bacteria Sphingomonas SaMR12:Effects on plant growth and root exudates[J]. Chemosphere, 2014, 117(1):367-373.
[3] Yuan M, He H, Xiao L, et al.Enhancement of Cd phytoextraction by two Amaranthus species with endophytic Rahnella sp. JN27[J]. Chemosphere, 2014, 103(5):99-104.
[4] Chen L, Luo SL, Li XJ, et al.Interaction of Cd-hyperaccumulator Solanum nigrum L. and functional endophyte Pseudomonas sp. Lk9 on soil heavy metals uptake[J]. Soil Biology & Biochemistry, 2014, 68(1):300-308.
[5] Zhang YF, He LY, Chen ZJ, et al.Characterization of ACC deaminase-producing endophytic bacteria isolated from copper-tolerant plants and their potential in promoting the growth and copper accumulation of Brassica napus[J]. Chemosphere, 2011, 83(1):57-62.
[6] Zhang WH, He LY, Wang Q, et al.Inoculation with endophytic Bacillus megaterium 1Y31 increases Mn accumulation and induces the growth and energy metabolism-related differentially-expressed proteome in Mn hyperaccumulator hybrid pennisetum[J]. Journal of Hazardous Materials, 2015, 300:513-521.
[7] VIsioli G, D’egidio S, Vamerali T, et al.Culturable endophytic bacteria enhance Ni translocation in the hyperaccumulator Noccaea caerulescens[J]. Chemosphere, 2014, 117(117C):538.
[8] Kamran MA, Bibi S, Xu RK, et al.Phyto-extraction of chromium and influence of plant growth promoting bacteria to enhance plant growth[J]. Journal of Geochemical Exploration, 2017, 182:269-274.
[9] Ma Y, Rajkumar M, Moreno A, et al.Serpentine endophytic bacterium Pseudomonas azotoformans ASS1 accelerates phytoremediation of soil metals under drought stress[J]. Chemosphere, 2017, 185:75-85.
[10] 杨波, 陈晏, 李霞, 等. 植物内生菌促进宿主氮吸收与代谢研究进展[J]. 生态学报, 2013, 33(9):2656-2664.
[11] 刘劲松, 张健君, 杨淑芳, 等. 内生菌参与植物/微生物联合修复重金属污染土壤的研究进展[J]. 中国植保导刊, 2014, 34(2):27-30.
[12] 王璐, 何琳燕, 盛下放. 耐铜苏丹草根内生细菌的分离筛选及其生物学特性研究[J]. 土壤, 2016, 48(1):95-101.
[13] Khan AL, Waqas M, Hussain J, et al.Endophytes Aspergillus caespitosus LK12 and Phoma sp. LK13 of Moringa peregrina produce gibberellins and improve rice plant growth[J]. Journal of Plant Interactions, 2014, 9(1):731-737.
[14] Todorovic B.Promotion of plant growth by bacterial ACC deaminase[J]. Critical Reviews in Plant Sciences, 2007, 26(5-6):227-242.
[15] Sun LN, Zhang YF, He LY, et al.Genetic diversity and characterization of heavy metal-resistant-endophytic bacteria from two copper-tolerant plant species on copper mine wasteland[J]. Bioresour Technol, 2010, 101(2):501-509.
[16] Zhang YF, He LY, Chen ZJ, et al.Characterization of lead-resistant and ACC deaminase-producing endophytic bacteria and their potential in promoting lead accumulation of rape[J]. Journal of Hazardous Materials, 2011, 186(2-3):1720.
[17] Seo DJ, Nguyen DM, Song YS, et al.Induction of defense response against Rhizoctonia solani in cucumber plants by endophytic bacterium Bacillus thuringiensis GS1.[J]. Journal of Microbiology & Biotechnology, 2012, 22(3):407.
[18] Afzal I, Shinwari ZK, Iqrar I.Selective isolation and characterization of agriculturally beneficial endophytic bacteria from wild hemp using canola[J]. Pakistan Journal of Botany, 2015, 47(5):1999-2008.
[19] Złoch M, Thiem D, Gadzała-Kopciuch R, et al.Synthesis of siderophores by plant-associated metallotolerant bacteria under exposure to Cd²+[J]. Chemosphere, 2016, 156:312-325.
[20] Rajkumar M, Ae N, Prasad MNV, et al.Potential of siderophore-producing bacteria for improving heavy metal phytoextraction[J]. Trends in Biotechnology, 2010, 28(3):142-149.
[21] 邓平香, 张馨, 龙新宪. 产酸内生菌荧光假单胞菌R1对东南景天生长和吸收、积累土壤中重金属锌镉的影响[J]. 环境工程学报, 2016, 10(9):5245-5254.
[22] Román-Ponce B, Ramos-Garza J, Arroyo-Herrera I, et al.Mechanism of arsenic resistance in endophytic bacteria isolated from endemic plant of mine tailings and their arsenophore production[J]. Archives of Microbiology, 2018, 200(6):883-895.
[23] Shin MN, Shim J, You Y, et al. Characterization of lead resistant endophytic Bacillus sp. MN3-4 and its potential for promoting lead accumulation in metal hyperaccumulator Alnus firma[J]. Journal of Hazardous Materials, 2012, 199-200(2):314.
[24] 赵希俊, 宋萍, 封磊, 等. 一株具有耐铝促生作用的茶树内生细菌的分离鉴定[J]. 江西农业大学学报, 2014, 36(2):407-412.
[25] Luo S, Wan Y, Xiao X, et al.Isolation and characterization of endophytic bacterium LRE07 from cadmium hyperaccumulator Solanum nigrum L. and its potential for remediation.[J]. Applied Microbiology & Biotechnology, 2011, 89(5):1637-1644.
[26] Dong DT, Yamaguchi N, Makino T, et al.Effect of soil microorganisms on arsenite oxidation in paddy soils under oxic conditions[J]. Soil Science & Plant Nutrition, 2014, 60(3):377-383.
[27] Ghosh P, Rathinasabapathi B, Teplitski M, et al.Bacterial ability in AsIII oxidation and AsV reduction:Relation to arsenic tolerance, P uptake, and siderophore production[J]. Chemosphere, 2015, 134(13):1.
[28] Fazi S, Crognale S, Casentini B, et al.The arsenite oxidation potential of native microbial communities from arsenic-rich freshwaters[J]. Microbial Ecology, 2016, 72(1):25-35.
[29] Wang L, Lin H, Dong Y, et al.Isolation of vanadium-resistance endophytic bacterium PRE01 from Pteris vittata in stone coal smelting district and characterization for potential use in phytoremediation[J]. Journal of Hazardous Materials, 2017, 341:1.
[30] Wang L, Lin H, Dong Y, et al.Isolation of vanadium-resistance endophytic bacterium PRE01 from Pteris vittata in stone coal smelting district and characterization for potential use in phytoremediation[J]. Journal of Hazardous Materials, 2017, 341:1-9.
[31] Tiwari S, Sarangi BK, Thul ST.Identification of arsenic resistant endophytic bacteria from Pteris vittata roots and characterization for arsenic remediation application[J]. Journal of Environmental Management, 2016, 180:359-365.
[32] Gao Y, Xia J, Mao L, et al.Effect of citric acid on phytoextraction and antioxidative defense in Solanum nigrum L. as a hyperaccumu-lator under Cd and Pb combined pollution[J]. Environmental Earth Sciences, 2012, 65(7):1923-1932.
[33] Khan AR, Ullah I, Khan AL, et al.Phytostabilization and physicochemical responses of korean ecotype Solanum nigrum L. to cadmium contamination[J]. Water Air & Soil Pollution, 2014, 225(10):2147.
[34] Yan Z, Zhang W, Chen J, et al.Methyl jasmonate alleviates cadmium toxicity in Solanum nigrum, by regulating metal uptake and antioxidative capacity[J]. Biologia Plantarum, 2015, 59(2):373-381.
[35] Tiwari S, Sarangi BK.Comparative analysis of antioxidant response by Pteris vittata, and Vetiveria zizanioides, towards arsenic stress[J]. Ecological Engineering, 2017, 100:211-218.
[36] Wan Y, Luo S, Chen J, et al.Effect of endophyte-infection on growth parameters and Cd-induced phytotoxicity of Cd-hyperaccumulator Solanum nigrum L.[J]. Chemosphere, 2012, 89(6):743-750.
[37] He H, Ye Z, Yang D, et al.Characterization of endophytic Rahnella sp. JN6 from Polygonum pubescens and its potential in promoting growth and Cd, Pb, Zn uptake by Brassica napus[J]. Chemosphere, 2013, 90(6):1960-1965.
[38] 刘莉华, 刘淑杰, 陈福明, 等. 接种内生细菌对龙葵吸收积累镉的影响[J]. 环境科学学报, 2013, 33(12):3368-3375.
[39] Ma Y, Rajkumar M, Moreno A, et al.Serpentine endophytic bacterium Pseudomonas azotoformans, ASS1 accelerates phytoremediation of soil metals under drought stress[J]. Chemosphere, 2017, 185:75-85.
[40] Schulz B, Boyle C.What are endophytes?[M] //Schulz BJE, Boyle CJC, Sieber TN. Microbial root endophytes. Berlin:Springer-Verlag, 2006:1-13.
[41] Hallmann J, Quadt-Hhallmann A, Mahaffee WF, et al.Bacterialendophytes in agricultural crops[J]. Can J Microbiol, 1997, 43:895-914.
[42] Sturz AV, Christie BR, Nowak J.Baterial endophytes:Potential role in developing sustainable systems of crop production[J]. Critical Reviews in Plant Sciences, 2000, 19(1):1-30.
[43] Bouchet V, Huot H, Goldstein R.Molecular genetic basis of ribotyping[J]. Clinical Microbiology Reviews, 2008, 21(2):262.
[44] 邓平香, 郭荣荣, 余光伟, 等. 超积累和非超积累生态型东南景天茎、叶内生细菌多样性分析[J]. 微生物学通报, 2017, 44(3):533-544.
[45] 何琳燕, 李娅, 刘涛, 等. 龙葵根际和内生Cd抗性细菌的筛选及其生物学特性[J]. 生态与农村环境学报, 2011, 27(6):83-88.
[46] Cai M, Song G, LI Y, et al.A novel Aroclor 1242-degrading culturable endophytic bacterium isolated from tissue culture seedlings of Salix matsudana f. pendula Schneid[J]. Phytochemistry Letters, 2018, 23:66-72.
[47] Nongkhlaw FMW, Joshi SR.Microscopic study on colonization and antimicrobial property of endophytic bacteria associated with ethnomedicinal plants of Meghalaya[J]. Journal of Microscopy and Ultrastructure, 2016, 5(3):132-139.
[48] Passari AK, Mshral VK, Leo VV, et al.Phytohormone production endowed with antagonistic potential and plant growth promoting abilities of culturable endophytic bacteria isolated from Clerodendrum colebrookianum Walp[J]. Microbiological Research, 2016, 193:57-73.
[49] Luo SL, Chen L, Chen JL, et al.Analysis and characterization of cultivable heavy metal-resistant bacterial endophytes isolated from Cd-hyperaccumulator Solanum nigrum L. and their potential use for phytoremediation[J]. Chemosphere, 2011, 85(7):1130.
[50] Románponce B, Ramosgarza J, Vásquezmurrieta MS, et al.Cultivable endophytic bacteria from heavy metal(loid)-tolerant plants.[J]. Archives of Microbiology, 2016, 198(10):941-956.
[51] Zhang X, Lin L, Zhu Z, et al.Colonization and modulation of host growth and metal uptake by endophytic bacteria of Sedum alfredii.[J]. International Journal of Phytoremediation, 2013, 15(1):51.