生物技术通报 ›› 2018, Vol. 34 ›› Issue (4): 174-178.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0073

• 研究报告 • 上一篇    下一篇

一株油菜菌核病拮抗真菌的鉴定及生物学特性研究

丁锐1, 张浩琪2, 3, 陈旭辉2, 林浩2, 曲波2   

  1. 1. 沈阳农业大学土地与环境学院,沈阳 110866;
    2. 沈阳农业大学生物科学技术学院,沈阳 110866;
    3. 南开大学生命科学学院,天津 300071
  • 收稿日期:2018-01-18 出版日期:2018-04-20 发布日期:2018-05-04
  • 作者简介:丁锐,男,博士,研究方向:微生物代谢工程;E-mail:enixer2002@163.com
  • 基金资助:
    国家自然科学基金项目(31670378),辽宁省自然科学基金项目(201602656)

Identification and Biological Characteristics of One Antagonistic Fungal Strain Against Sclerotinia sclerotiorum

DING Rui1, ZHANG Hao-qi2, 3, CHEN Xu-hui2, LIN Hao2, QU Bo2   

  1. 1. College of Land and Environment,Shenyang Agricultural University,Shenyang 110866;
    2. College of Bioscience and Biotechnology,Shenyang Agricultural University,Shenyang 110866;
    3. College of Life Science,Nankai University,Tianjin 300071
  • Received:2018-01-18 Published:2018-04-20 Online:2018-05-04

摘要: 从兰科植物羊耳蒜根部分离并筛选对油菜菌核病菌Sclerotinia sclerotiorum 具有较强拮抗作用的内生真菌,确定其分类地位,并对其体外培养特性进行研究。利用常规PDA培养法进行内生真菌的分离、培养,采用平板对峙法筛选具有较强抑菌活性的菌株并对其进行种类鉴定,同时设定不同碳源、氮源、温度和pH值条件研究该菌株的生物学特性。通过筛选获得1株对油菜菌核病菌具有较强抑制作用的真菌菌株,结合形态学特征和ITS分子序列分析将其鉴定为多节孢属Nodulisporium sp.。该菌株对碳源要求不严格,以麦芽糖、果糖、葡萄糖和蔗糖为碳源均能良好生长。氮源对该菌株生长具有显著影响,最适氮源为牛肉膏。其最适培养温度为25℃,且温度低于10℃或高于30℃时菌丝生长均受到明显抑制。同时该菌株能耐受较广的酸碱度范围,在pH5-9范围内均生长良好。

关键词: 油菜菌核病, 生物防治, 体外培养, 内生真菌, 兰科

Abstract: Our aims are to isolate and select antagonistic endophytic fungi against Sclerotinia sclerotionum from the roots of Liparis japonica(Orchidaceae),to identify their taxonomic status and study the characteristics of in vitro culturing it. Conventional PDA culture technology was applied to isolate and culture endophytic fungi and plate confrontation method to screen those strains with strong antagonistic activities against S. sclerotionum and to identify their taxonomic status,as well as the biological characteristics were studied under different carbon sources,nitrogen sources,temperature and pH values. One strongly antagonistic endophytic fungus against S. sclerotionum were obtained and identified as Nodulisporium sp. by its morphological characters and ITS molecular sequences. The carbon sources that this fungus relied on were diverse and it grew well by any of maltose,fructose,glucose and sucrose as carbon resource. There existed significant differences among nitrogen sources for this fungus’ growth,and the optimal nitrogen source was beef extract. The optimal temperature was 25℃,and the hypha was inhibited significantly when the temperature fell below 10℃or rose above 30℃. This fungus also tolerated very wide range of pH,and grew well under pH 5 to 9.

Key words: Sclerotinia rot of colza, biological control, culture in vitro, endophytic fungi, Orchidaceae