锰、砷对地衣芽孢杆菌铀富集的影响

doi:10.13560/j.cnki.biotech.bull.1985.2017-0992

摘要/Abstract

摘要： 为研究地衣芽孢杆菌对铀（U）、锰（Mn）和砷（As）的吸附特性，以及Mn、As对地衣芽孢杆菌对U吸附的影响，为该菌在U污染治理中的应用提供理论支持。通过单因素及多因素试验，研究了地衣芽孢杆菌对U、Mn、As的富集规律及Mn、As对U富集的影响，并利用红外光谱（FTIR）分析其影响机理。研究表明：单因素实验中，U浓度大于75 mg/L、Mn浓度大于25 mg/L或As浓度大于50 mg/L均会对地衣芽孢杆菌的生长产生明显的抑制；同时，U、Mn浓度在0-100 mg/L范围内其浓度与吸附率呈负相关，As在0-100 mg/L范围内其吸附率先降低后上升的趋势，且100 mg/L时吸附率最高，达到79.73%。多因素实验发现，As浓度低于60 mg/L时促进地衣芽孢杆菌对U的富集，高于60 mg/L发生抑制作用。低于40 mg/L的Mn促进芽孢杆菌对U的吸附，浓度高于60 mg/L Mn对其U的吸附产生抑制作用。Mn和As通过抑制细菌生长、竞争菌体表面负电性基团、改变菌体物质特性等方式影响菌体对U的吸附。

关键词: 地衣芽孢杆菌, 吸附, 锰, 砷, 微生物修复

Abstract: This work is to understand the adsorption characteristics of uranium（U），manganese（Mn），and arsenic（As）to Bacillus licheniformis and the effects of Mn and As on the adsorption of uranium to B. licheniformis，for providing theoretical supports while applying this bacterium in the control of uranium pollution. Enrichment laws of B. licheniformis to uranium，manganese and arsenic，as well as the influences of manganese and arsenic on uranium enrichment were studied by single-factor and multiple-factor experiments. Also，the Fourier Transform Infrared Spectra（FTIR）was used to analyze the influencing mechanism. The research showed that all of them obviously inhibited the growth of B. licheniformis when the concentration of uranium，manganese，and arsenic were over 75 mg/L，25 mg/L，and 25 mg/L，respectively. Meanwhile，the concentration of uranium and manganese was negatively correlated to the adsorption rate respectively when the concentration was in 0-100 mg/L. However，the arsenic adsorption decreased at first and then rose when arsenic concentration was in 0-100 mg/L. The maximum adsorption rate of arsenic reached the highest of 79.73% while its concentration was 100 mg/L. Multiple-factor experiments suggested that arsenic promoted the uranium enrichment of B. licheniformis when its concentration was < 60 mg/L，while inhibition occurred when its concentration was > 60 mg/L. Moreover，manganese at the concentration of < 40 mg/L promoted the enrichment uranium of B. licheniformis，but inhibited when the concentration was > 60 mg/L. Manganese and arsenic affect bacteria on uranium adsorption in form of restraining bacteria growth as well as competing bacteria surface negative groups and converting properties of bacteria.

Key words: Bacillus licheniformis, adsorption, manganese, arsenic, microbial remediation

望子龙 ,罗学刚 ,司慧 ,王焯. 锰、砷对地衣芽孢杆菌铀富集的影响[J]. 生物技术通报, 2018, 34(6): 164-171.

WANG Zi-long ,LUO Xue-gang ,SI Hui ,WANG Zhuo. Effects of Manganese and Arsenic on Uranium Enrichment of Bacillus licheniformis[J]. Biotechnology Bulletin, 2018, 34(6): 164-171.

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