中华鳖MyoD1基因SNP鉴定及其与生长性状的关联分析

doi:10.13560/j.cnki.biotech.bull.1985.2018-0907

生物技术通报 ›› 2019, Vol. 35 ›› Issue (4): 76-81.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0907

中华鳖MyoD1基因SNP鉴定及其与生长性状的关联分析

黄龙, 吴本丽, 何吉祥, 陈静, 宋光同, 汪翔, 张烨, 武松

1. 安徽省农业科学院水产研究所,合肥 230031;
2. 水产增养殖安徽省重点实验室,合肥 230031

收稿日期:2018-10-22 出版日期:2019-04-26 发布日期:2019-05-05
作者简介:黄龙,男,博士,助理研究员,研究方向：动物遗传育种与繁殖;E-mail：huanglong26@126.com
基金资助:
安徽省农业科学院院长青年创新基金（17B0509）,安徽省科技攻关计划项目（1604a0702018）,安徽省重点研究与开发计划项目（1704g07020118）

SNP Identification of MyoD1 Gene and Its Correlation with Growth Traits in Pelodiscus sinensis

HUANG Long, WU Ben-li, HE Ji-xiang, CHEN Jing, SONG Guang-tong, WANG Xiang, ZHANG Ye, WU Song

1. Fisher Research Institute,Anhui Academy of Agricultural Sciences,Hefei 230031;
2. Key Laboratory of Aquaculture & Stock Enhancement for Anhui Province,Hefei 230031;

Received:2018-10-22 Published:2019-04-26 Online:2019-05-05

摘要/Abstract

摘要： 为了研究生肌决定因子1（MyoD1）基因多态性与中华鳖生长性状的相关性,采用直接测序法在MyoD1基因上共检测到6个SNP位点（T-49G、A-38G、C91T、A187T、C880T和T1522A）,其中C880T位于外显子上,属于错义突变。对从同批繁殖、同块稻田养殖的2冬龄中华鳖群体中随机选取的178只个体中各位点的基因型进行检测。结果显示,所有位点在中华鳖群体中的平均有效等位基因数、平均观测杂合度和平均期望杂合度分别为1.636 5、0.349 3和0.375 4,除A187T 位点外,其余5个位点的基因型频率分布均符合Hardy-Weinberg定律。采用一般线性模型分析各位点与中华鳖生长性状之间的相关性,研究发现,T-49G位点GG基因型个体的背甲宽显著大于TT基因型,A-38G位点AG基因型个体的背甲宽显著大于AA基因型,A187T位点TT基因型个体的体高显著大于AA基因型,T1522A位点AA基因型个体的体质量显著大于TT、TA基因型,其余位点不同基因型个体间的生长性状均不存在显著差异。T-49G、A-38G、A187T和T1522A位点与生长性状显著相关,可作为中华鳖分子标记辅助育种的候选标记。

关键词: 中华鳖, 生肌决定因子1基因, 单核苷酸多态性标记, 生长性状, 关联分析

Abstract: In order to study the correlation between DNA polymorphisms of MyoD1 gene and growth traits of P. sinensis,direct sequencing method was used and 6 SNP loci（T-49G,A-38G,C91T,A187T,C880T and T1522A）in P. sinensis MyoD1 gene were identified. Among them,one SNP（C880T）was located in exons and missense mutation happened. Randomly selecting 178 randomly 2-winter-age P. sinensis bred in the same batch and cultivated in the same rice field,the genotypes of each SNP locus of them were detected. The results showed that the average effective number of allele（Ne）,average observed heterozygosity（Ho）,and average expected heterozygosity（He）of these SNPs was 1.636 5,0.349 3 and 0.375 4,respectively. Chi-square test showed that 5 SNP loci（T-49G,A-38G,C91T,C880T,and T1522A）were in Hardy-Weinberg equilibrium state in P. sinensis population. A general linear model was established for correlation analysis between different genotypes at SNP loci of MyoD1 gene and various growth traits. At the locus of T-49G,individuals with the GG genotype had significantly greater carapace widths than ones with the TT genotypes. At the locus of A-38G,individuals with the AG genotype had significantly greater carapace widths than ones with the AA genotypes. At the locus of A187T,individuals with the TT genotype had significantly greater heights than ones with the genotype AA. At the locus of T1522A,individuals with the AA genotype had significantly greater body weights than ones with the TT and TA genotypes. Other three SNP loci were not significantly correlated with growth traits. The growth-related locus T-49G,A-38G,A187T and T1522A could be useful candidate molecular markers in P. sinensis molecular marker-assisted selection.

Key words: Pelodiscus sinensis, myogenic differentiation 1 gene, single nucleotide polymorphism, growth traits, correlation analysis

黄龙, 吴本丽, 何吉祥, 陈静, 宋光同, 汪翔, 张烨, 武松. 中华鳖MyoD1基因SNP鉴定及其与生长性状的关联分析[J]. 生物技术通报, 2019, 35(4): 76-81.

HUANG Long, WU Ben-li, HE Ji-xiang, CHEN Jing, SONG Guang-tong, WANG Xiang, ZHANG Ye, WU Song. SNP Identification of MyoD1 Gene and Its Correlation with Growth Traits in Pelodiscus sinensis[J]. Biotechnology Bulletin, 2019, 35(4): 76-81.

参考文献

[1] 张群英. 基于转录组测序的中华鳖微卫星标记的开发及MSTN基因的克隆表达[D]. 苏州:苏州大学, 2014.
[2] Zhang H, Xu X, He Z, et al.De novo transcriptome analysis reveals insights into different mechanisms of growth and immunity in a Chinese soft-shelled turtle hybrid and the parental varieties[J]. Gene, 2016, 605:54-62.
[3] 彭娜, 曾丹, 王晓清, 等. 中华鳖IGF2基因SNP标记与生长性状的关联分析[J]. 湖南农业大学学报:自然科学版, 2018, 44(1):88-94.
[4] 李纯, 陈辰, 赵建, 等. 中华鳖GHRL基因SNPs的筛选及生长性状的关联分析[J]. 水生生物学报, 2018, 42(2):307-312.
[5] Buckingham M.Making muscle in mammals[J]. Trends in Genetics, 1992, 8(4):144-149.
[6] Ferri P, Barbieri E, Burattini S, et al.Expression and subcellular localization of myogenic regulatory factors during the differentiation of skeletal muscle C₂C1₂ myoblasts[J]. Journal of Cellular Biochemistry, 2009, 108(6), 1302-1317.
[7] Weintraub H.The MyoD family and myogenesis:redundancy, networks, and thresholds[J]. Cell, 1993, 75(7):1241-1244.
[8] Tan X, Du SJ.Differential expression of two MyoD genes in fast and slow muscles of gilthead seabream(Sparusaurata)[J]. Development Genes & Evolution, 2002, 212(5):207-217.
[9] Hall TE, Cole NJ, Johnston IA.Temperature and the expression of seven muscle-specific protein genes during embryogenesis in the Atlantic cod Gadus morhua L.[J]. Journal of Experimental Biology, 2003, 206(18):3187.
[10] Urano A, Suzuki MM, Zhang P, et al.Expression of muscle-related genes and two MyoD genes during amphioxus notochord development[J]. Evolution and Development, 2010, 5(5):447-458.
[11] 梁宏伟, 李忠, 邹桂伟, 等. 黄颡鱼MyoD基因的克隆及其表达[J]. 武汉大学学报:理学版, 2012, 58(4):347-353.
[12] 李玉珑, 农小献, 李虹辉, 等. 鳜鱼生肌调节因子MyoD的克隆及其发育表达分析[J]. 湖南师范大学自然科学学报, 2013, 36(4):63-68.
[13] 晁珊珊, 张倡珲, 海蕾, 等. 长丝裂腹鱼MyoD1基因的克隆及序列分析[J]. 安徽农业科学, 2013, 41(11):4750-4752.
[14] Zhao Z, Fu YX, Hewett-Emmett D, et al.Investigating single nucleotide polymorphism(SNP)density in the human genome and its implications for molecular evolution[J]. Gene, 2003, 312:207-213.
[15] 樊佳佳, 刘小献, 白俊杰, 等. 草鱼柠檬酸合酶基因SNP筛选及与生长性状的关联分析[J]. 华中农业大学学报, 2014, 33(3):84-89.
[16] Nie Q, Lei M, Ouyang J, et al.Identification and characterization of single nucleotide polymorphisms in 12 chicken growth-correlated genes by denaturing high performance liquid chromatography[J]. Genetics Selection Evolution, 2005, 37(3):339-360.
[17] Yang ZH, Zhou CL, Zhu H, et al.A functional SNP in the MDM2 promoter mediates E2F1 affinity to modulate cyclin D1 expression in tumor cell proliferation[J]. Asian Pacific Journal of Cancer Prevention, 2014, 15(8):3817-3823.
[18] Wang L, Li K, Xu Q, et al.Potential synergy between SNP and CpG-A or IL-1β in regulating transcriptional activity of IL-20 promoter[J]. Journal of Investigative Dermatology, 2014, 134(2):389-395.
[19] 李胜杰, 姜鹏, 樊佳佳, 等. 大口黑鲈肌球蛋白重链基因SNPs的筛选及与生长性状的关联[J]. 水产学报, 2018, 42(3):305-313.
[20] 项露颉, 姜怀志, 马龙, 等. MyoD基因在不同品种肉羊肌肉中表达比较[J]. 中国兽医学报, 2018, 38(1):210-216.
[21] Zhou D, Xu HQ, Chen W, et al.Study on the transcriptional regulatory mechanism of the MyoD1 gene in Guanling bovine[J]. Rsc Advances, 2018, 8(22):12409-12419.
[22] Hinits Y, Williams VC, Sweetman D, et al.Defective cranial skeletal development, larval lethality and haplo in sufficiency in Myod mutant zebrafish[J]. Developmental Biology, 2011, 358(1):102-112.
[23] 于凌云, 白俊杰, 叶星, 等. 大口黑鲈MyoD基因结构和单核苷酸多态性位点的筛选[J]. 水产学报, 2009, 33(1):1-8.
[24] 陈松波, 龚丽, 范兆廷, 等. 牙鲆MyoD基因单核苷酸多态性位点的筛选[J]. 水生生物学报, 2013, 37(5):863-868.
[25] Chen WX, Ma Y, Liu KH.Association of MyoD1a and MyoD1b gene polymorphisms and meat quality traits in rainbow trout[J]. Genetics and Molecular Research, 2015, 14(3):9034-9044.
[26] 陈静, 何吉祥, 樊佳佳, 等. 草鱼MyoD基因SNP和Indel标记的筛选及其与生长性状的关联分析[J]. 江苏农业学报, 2018, 34(3):635-639.

中华鳖MyoD1基因SNP鉴定及其与生长性状的关联分析

SNP Identification of MyoD1 Gene and Its Correlation with Growth Traits in Pelodiscus sinensis

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 8

编辑推荐

Metrics

本文评价

[1]	肖小军, 陈明, 韩德鹏, 余跑兰, 郑伟, 肖国滨, 周庆红, 周会汶. 甘蓝型油菜每角果粒数全基因组关联分析[J]. 生物技术通报, 2023, 39(3): 143-151.
[2]	张立兴, 王丽娜, 康广博, 黄鹤. 多组学分析在炎症性肠病中的应用与研究进展[J]. 生物技术通报, 2021, 37(1): 155-167.
[3]	任善茂, 王温慧, 陶勇. 苏姜猪OLR1基因多态性及其与肉质性状的关联分析[J]. 生物技术通报, 2020, 36(5): 145-149.
[4]	李标, 张瑞莹, 王小琪, 张存芳, 段子渊. 滩羊微卫星标记多态性及与体尺性状关联分析[J]. 生物技术通报, 2019, 35(6): 131-137.
[5]	张瑞, 郎侠, 吴建平, 王彩莲, 梁婷玉. 岷县黑裘皮羊微卫星多态性及与生产性状的关联分析[J]. 生物技术通报, 2019, 35(11): 55-63.
[6]	王燕新, 廖圆圆, 阿依木古丽, 齐骜穹, 李海健, 徐红伟, 杨具田, 蔡勇. 三个绵羊品种LHX3基因多态性与生长性状的关联分析[J]. 生物技术通报, 2019, 35(10): 162-168.
[7]	高丽丽, 刁晓明, 李云, 翟旭亮, 周春龙. 中华鳖性别决定中主导性因素的研究进展及思考[J]. 生物技术通报, 2018, 34(12): 41-49.
[8]	张雁明, 邢国芳, 刘美桃, 刘晓东, 韩渊怀. 全基因组关联分析: 基因组学研究的机遇与挑战[J]. 生物技术通报, 2013, 0(6): 1-6.