嗜水气单胞菌OprM蛋白的克隆表达与免疫保护作用评价

doi:10.13560/j.cnki.biotech.bull.1985.2019-0319

生物技术通报 ›› 2019, Vol. 35 ›› Issue (9): 244-248.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0319

嗜水气单胞菌OprM蛋白的克隆表达与免疫保护作用评价

毛然然, 李小艳, 武瑶, 张丽珊, 林镇平, 林向民

1. 福建农林大学生命科学学院,福州 350002;
2. 福建农林大学福建省农业生态过程与安全监控重点实验室,福州 350002

收稿日期:2019-04-17 出版日期:2019-09-26 发布日期:2019-09-16
作者简介:毛然然,男,研究方向：微生物分子生态学;E-mail：2961640381@qq.com
基金资助:
国家自然科学基金面上项目（31670129）

Cloning and Expression of Outer Membrane Protein OprM from Aeromonas hydrophila and the Evaluation of Its Immunoprotective Effect

MAO Ran-ran, LI Xiao-yan, WU Yao, ZHANG Li-shan, LIN Zhen-ping, LIN Xiang-min

1. School of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002;
2. Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring,Fujian Agriculture and Forestry University,Fuzhou 350002

Received:2019-04-17 Published:2019-09-26 Online:2019-09-16

摘要/Abstract

摘要： 嗜水气单胞菌（Aeromonas hydrophila）是一种常见的条件致病菌,控制该菌在水产养殖业中的危害是当前亟待解决的问题。利用克隆表达等分子生物学手段,获得嗜水气单胞菌 ATCC 7966 OprM 纯化蛋白,肌肉注射斑马鱼后对其免疫相关基因的转录水平进行检测,探索其免疫应答效果。结果显示大多数免疫相关基因表达上调,表明OprM蛋白在斑马鱼体内能够产生较好的免疫应答。进一步利用嗜水气单胞菌强毒株LP-2对免疫后斑马鱼进行攻毒实验,发现注射OprM蛋白后相对免疫保护率达89.47%,以上结果表明嗜水气单胞菌外膜蛋白OprM对斑马鱼具有较高的免疫保护性,是一种潜在的嗜水气单胞菌候选疫苗。

关键词: 嗜水气单胞菌, 外膜蛋白OprM, 免疫原性, 免疫应答

Abstract: Aeromonas hydrophila is a common conditional pathogen. Controlling the damage of this bacterium in aquaculture industry is an urgent problem to be solved. Hereby the purified protein of A. hydrophila ATCC 7966 OprM was obtained by molecular biology methods such as cloning and expression. After intramuscular injection of the purified protein to zebrafish,the transcription level of immune-related genes was detected and the immune response was explored. The results showed that most of the immune-related genes were up-regulated,indicating that OprM protein evoked a considerable immune responses in zebrafish. Further velogenic strain A. hydrophila LP-2 was used to challenge the immunized zebrafish,and it was revealed that the relative immune protection rate after the injection of OprM protein reached 89.47%,the above results indicate outer membrane protein OprM is highly immunoprotective against zebrafish and is a potential vaccine candidate for A. hydrophila.

Key words: Aeromonas hydrophila, outer membrane protein OprM, immunogenicity, immune response

毛然然, 李小艳, 武瑶, 张丽珊, 林镇平, 林向民. 嗜水气单胞菌OprM蛋白的克隆表达与免疫保护作用评价[J]. 生物技术通报, 2019, 35(9): 244-248.

MAO Ran-ran, LI Xiao-yan, WU Yao, ZHANG Li-shan, LIN Zhen-ping, LIN Xiang-min. Cloning and Expression of Outer Membrane Protein OprM from Aeromonas hydrophila and the Evaluation of Its Immunoprotective Effect[J]. Biotechnology Bulletin, 2019, 35(9): 244-248.

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嗜水气单胞菌OprM蛋白的克隆表达与免疫保护作用评价

Cloning and Expression of Outer Membrane Protein OprM from Aeromonas hydrophila and the Evaluation of Its Immunoprotective Effect

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