生境、温度及外源激素对花花柴花器官发育的影响

doi:10.13560/j.cnki.biotech.bull.1985.2020-1126

摘要/Abstract

摘要：

植物花器官的生长发育与温度密切相关,高温会导致花器官畸形及体内激素含量的变化。为探究不同生境配合外施生长素（IAA）对花花柴（Karelinia caspia）花器官发育的影响,测定沙漠和人工绿地2种环境下及外施不同浓度生长素（IAA）的花花柴花器官大小（雌花花柱、雄花花丝）及花器官内激素含量的变化。结果表明,在花苞期合适的温度（27-30℃）能够促进花花柴花器官的生长,而在开花过程中,高温（38-40℃）可促进花花柴柱头伸长进而影响花器官生长,直至发育成熟。外施一定浓度的IAA对花花柴花器官的生长具有促进作用,沙漠环境中,外施0.3 μmol/L的IAA促进作用最明显而人工绿地则为0.1 μmol/L。

关键词: 高温胁迫, 花花柴, 外源激素

Abstract:

The growth and development of flower organs in plants are closely related to temperature,and high temperature can cause flower organs abnormal and changes in hormone content in the plant. To explore the effects of different habitats and exogenous IAA on the floral organ development of Karelinia caspia,the floral organ size of K. caspia（Female style and male filaments）under 2 habitats of desert and artificial green space and at different concentrations of IAA as well as the changes of hormone content in the floral organ were measured. Results showed that the appropriate temperature（27-30℃）promoted the growth of K. caspia flowering organs in the bud stage,while the high temperature（38-40℃）promoted the elongation of K. caspia stigma during flowering,thus affecting the growth of flower organs until maturity. External application of IAA at a certain concentration promoted the growth of K. caspia flower organs. In the desert,the IAA promotion effect of 0.3 μmol/L was the most obvious,while that in the artificial green space was 0.1 μmol/L.

Key words: high temperature stress, Karelinia caspia, exogenous hormones

毕愿坤, 李丽, 朱传应, 王彦芹. 生境、温度及外源激素对花花柴花器官发育的影响[J]. 生物技术通报, 2021, 37(4): 28-34.

BI Yuan-kun, LI Li, ZHU Chuan-ying, WANG Yan-qin. Effects of Habitat,Temperature and Exogenous Hormones on the Organ Development of Karelinia caspia[J]. Biotechnology Bulletin, 2021, 37(4): 28-34.

图/表 4

图1 不同生境条件下花花柴花器官的发育及生长速度 A ：花花柴小花不同时期发育图;B ：花花柴整花不同时期发育图;C ：不同生境下花花柴雌蕊、雄蕊长度测量统计结果;D ：不同生境下花花柴花器官生长速率图。* 表示P<0.05 差异显著。下同

Fig. 1 Development and growth rate of floral organs in K. caspia under different habitat conditions A : Development of florets at different stages of K. caspia. B : Development of whole flower at different stages of K. caspia. C : Statistic measurement of pistil and stamen length of K. caspia in different habitats. D : Figure of floral organ growth rate of K. caspia in different habitats. * : P<0.05 significant difference. The same below

图2 不同生境条件下外施IAA 雌蕊、雄蕊的测量长度 A：沙漠环境外施IAA 雌蕊、雄蕊的测量长度;B：人工绿地环境外施IAA 雌蕊、雄蕊的测量长度。a ：CK ;b ：0.1 μmol/L ;c ：0.3 μmol/L ;d ：0.5 μmol/L

Fig.2 Measure the length of pistil and stamen while IAA applied in different habitats A : Measuring the length of pistil and stamen of IAA applied in desert environment. B : Measuring the length of pistil and stamen of IAA applied outside artificial green environment. a : CK, b : 0.1 μmol/L, c : 0.3 μmol/L, d : 0.5 μmol/L

图3 不同生境条件下花花柴花器官内IAA 含量的测定

Fig.3 Determination of IAA content in the floral organs of K. caspia under different habitat conditions **P<0.01

图4 外施不同浓度IAA 花花柴花器官内激素含量的测定 A：外施不同浓度IAA 对花花柴花器官内激素含量的影响;B ：人工绿地中外施不同浓度IAA 花花柴花器官内激素含量变化;C ：沙漠中外施不同浓度IAA 花花柴花器官内激素含量变化

Fig.4 Determination of hormone content in flower organs of K. caspia with different concentration of IAA applied externally A : Effects of different concentrations of IAA on hormone content in floral organs of K. caspia. B : Changes of hormone content in flower organs of K. caspia with different concentrations of IAA applied to artificial green space. C: Changes of hormone content in floral organs of K. caspia with different concentration of IAA applied in desert

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