基于SSR标记的马铃薯野生种和地方种遗传多样性分析和指纹图谱构建

doi:10.13560/j.cnki.biotech.bull.1985.2025-0142

摘要/Abstract

摘要：

目的从分子水平分析马铃薯野生种、地方种的遗传多样性并建立马铃薯种质资源的指纹图谱库。方法利用简单串接重复序列（simple sequence repeat, SSR）分子标记技术，对国际马铃薯中心亚太中心保存的73份马铃薯野生种、地方种的遗传多样性进行研究。结果平均等位基因数（Na）为1.843 7，Nei’s平均基因多样性指数为0.065 9，平均Shannon信息指数为0.128 6，多态性信息含量PIC平均值为0.848 4，表明供试的73份马铃薯种质资源多态性高但遗传差异小。Structure群体结构将73份马铃薯种质分为7个类群，UPGMA聚类分析分为4个类群。结论从14对引物中筛选出可完全区分73份种质的5对核心引物，构建了73份马铃薯野生种和地方种的指纹图谱，为后续马铃薯种质鉴定和筛选体系的研究提供了理论基础和技术支撑。

关键词: 马铃薯, 野生种, 地方种, 遗传多样性, SSR, 指纹图谱

Abstract:

Objective Analyze genetic diversity of wild and landrace potato species at the molecular level and establish a fingerprint database for potato germplasm resources. Method SSR (Simple Sequence Repeat) molecular markers was used to analyze the genetic diversity of 73 wild potato and landrace potato species preserved at the CIP-China Center for Asia Pacific. Result The genetic diversity analysis revealed an average number of alleles (Na) of 1.843 7, Nei’s gene diversity index of 0.065 9, Shannon’s information index of 0.128 6, and an average polymorphism information content (PIC) value of 0.848 4, indicating high polymorphism but low genetic differentiation among the 73 germplasm resources. The germplasms were classified into seven subpopulations by Structure analysis, whereas they were divided into four groups via UPGMA clustering. Conclusion Five pairs of core primers are selected from 14 SSR primers, enabling complete differentiation of all 73 accessions. These primers were used to construct unique DNA fingerprints for the 73 wild and landrace potato accessions. This work provides a theoretical foundation and technical framework for future germplasm identification and screening systems in potato breeding.

Key words: potato, wild potato, landraces, genetic diversity, SSR (Simple Sequence Repeat), fingerprinting

卢瑶, 袁平平, 金鑫, 毛向红, 范向斌, 白小东. 基于SSR标记的马铃薯野生种和地方种遗传多样性分析和指纹图谱构建[J]. 生物技术通报, 2025, 41(9): 94-104.

LU Yao, YUAN Ping-ping, JIN Xin, MAO Xiang-hong, FAN Xiang-bin, BAI Xiao-dong. Genetic Diversity Analysis and Fingerprinting of Wild Potato and Landraces Based on SSR Markers[J]. Biotechnology Bulletin, 2025, 41(9): 94-104.

图/表 9

参考文献 39

[1]	Food and Agriculture Organization of the United Nations. https://www.fao.org/faostat/en/#data/QCL [DB/OL]. 2022.
[2]	Devaux A, Goffart JP, Kromann P, et al. The potato of the future: opportunities and challenges in sustainable agri-food systems [J]. Potato Res, 2021, 64(4): 681-720.
[3]	宋波涛, 司怀军, 聂碧华. 马铃薯种质资源利用与改良 [M]. 呼和浩特: 内蒙古人民出版社, 2024.
	Song BT, Si HJ, Nie BH. Utilization and improvement of potato germplasm resources [M]. Hohhot: China: Inner Mongolian People’s Publishing House, 2024.
[4]	Spooner DM, Ghislain M, Simon R, et al. Systematics, diversity, genetics, and evolution of wild and cultivated potatoes [J]. Bot Rev, 2014, 80(4): 283-383.
[5]	Jenderek MM, Ambruzs BD, Tanner JD, et al. High regrowth of potato crop wild relative genotypes after cryogenic storage [J]. Cryobiology, 2023, 111: 84-88.
[6]	段绍光, 金黎平, 李广存, 等. 马铃薯品种遗传多样性分析 [J]. 作物学报, 2017, 43 (5): 718-729.
	Duan SG, Jin LP, Li GC, et al. Analysis of genetic diversity of potato varieties [J]. Acta Agronomica Sinica, 2017, 43 (5): 718-729.
[7]	Ali J, Sobhy IS, Bruce TJ. Wild potato ancestors as potential sources of resistance to the aphid Myzus persicae [J]. Pest Manag Sci, 2022, 78(9): 3931-3938.
[8]	Gorzolka K, Perino EHB, Lederer S, et al. Lysophosphatidylcholine 17: 1 from the leaf surface of the wild potato species Solanum bulbocastanum inhibits Phytophthora infestans [J]. J Agric Food Chem, 2021, 69(20): 5607-5617.
[9]	Xu Y, Gray SM. Aphids and their transmitted potato viruses: a continuous challenges in potato crops [J]. J Integr Agric, 2020, 19(2): 367-375.
[10]	Karki HS, Jansky SH, Halterman DA. Screening of wild potatoes identifies new sources of late blight resistance [J]. Plant Dis, 2021, 105(2): 368-376.
[11]	Hawkes JG. The potato: evolution, biodiversity and genetic resources [M]. Washington, D.C.: Smithsonian Institution Press, 1990.
[12]	Spooner DM, Rodríguez F, Polgár Z, et al. Genomic origins of potato polyploids: GBSSI gene sequencing data [J]. Crop Sci, 2008, 48(S1): 27-36.
[13]	Li YP, Colleoni C, Zhang JJ, et al. Genomic analyses yield markers for identifying agronomically important genes in potato [J]. Mol Plant, 2018, 11(3): 473-484.
[14]	宋晓燕, 张春芝, 李颖, 等. 二倍体马铃薯栽培品种遗传多样性的SSR标记分析 [J]. 园艺学报, 2016, 43 (11): 2266-2276.
	Song XY, Zhang CZ, Li Y, et al. SSR marker analysis of genetic diversity in diploid potato cultivars [J]. Acta Horticulturae Sinica, 2016, 43 (11): 2266-2276.
[15]	曹越, 张立媛, 辛旭霞, 等. 基于荧光SSR的宁夏糜子DNA分子身份证的构建 [J]. 作物学报, 2024, 50 (11): 2699-2711.
	Cao Y, Zhang LY, Xin XX, et al. Construction of DNA molecular identity card of proso millet in Ningxia based on fluorescent SSR [J]. Acta Agronomica Sinica, 2024, 50 (11): 2699-2711.
[16]	王秀军, 赵彦贝, 王静, 等. 基于SSR分子标记的175份蜡梅种质遗传多样性分析和指纹图谱构建 [J]. 生物工程学报, 2024, 40(1): 252-268.
	Wang XJ, Zhao YB, Wang J, et al. Genetic diversity analysis and fingerprinting of 175 Chimonanthus praecox germplasm based on SSR molecular marker [J]. Chin J Biotechnol, 2024, 40(1): 252-268.
[17]	韩嘉琪, 王海平, 宋江萍, 等. 基于SSR标记的百合资源遗传背景分析 [J]. 生物工程学报, 2024, 40(4): 1211-1224.
	Han JQ, Wang HP, Song JP, et al. Genetic background of lily germplasm resources based on SSR markers [J]. China Ind Econ, 2024, 40(4): 1211-1224.
[18]	王晓映, 张方玉, 万星, 等. 基于分子标记和表型性状的水稻地方品种遗传多样性研究 [J]. 植物遗传资源学报, 2023, 24 (3): 636-647.
	Wang XY, Zhang FY, Wan X, et al. Genetic diversity of local varieties of rice based on molecular markers and phenotypic traits [J]. Journal of Plant Genetic Resources, 2023, 24(3):636-647.
[19]	Tillault AS, Yevtushenko DP. Simple sequence repeat analysis of new potato varieties developed in Alberta, Canada [J]. Plant Direct, 2019, 3(6): e00140.
[20]	孙小琼, 王芳, 王崇, 等. 马铃薯品种(系)资源的SSR遗传多样性分析及指纹图谱构建 [J/OL]. 分子植物育种, 2022: 1-27.
	Sun XQ, Wang F, Wang C, et al. SSR genetic diversity analysis and fingerprinting of potato variety (line) resources [J/OL]. Mol Plant Breed, 2022: 1-27.
[21]	Achakkagari SR, Bozan I, Camargo-Tavares JC, et al. The phased Solanum okadae genome and Petota pangenome analysis of 23 other potato wild relatives and hybrids [J]. Sci Data, 2024, 11(1): 454.
[22]	Hosaka AJ, Sanetomo R, Hosaka K. A de novo genome assembly of Solanum bulbocastanum Dun., a Mexican diploid species reproductively isolated from the A-genome species, including cultivated potatoes [J]. G3, 2024, 14(6): jkae080.
[23]	Hardigan MA, Bamberg J, Robin Buell C, et al. Taxonomy and genetic differentiation among wild and cultivated germplasm of Solanum sect. petota [J]. Plant Genome, 2015, 8(1): eplantgenome2014.06.0025.
[24]	毛向红, 卢瑶, 范向斌, 等. 基于SSR荧光标记毛细管电泳的马铃薯品种遗传多样性分析及分子身份证构建 [J]. 生物技术通报, 2024, (9): 131-140.
	Mao XH, Lu Y, Fan XB, et al. Genetic diversity analysis of potato varieties based on SSR fluorescent marker capillary electrophoresis and construction of molecular identity card [J]. China Ind Econ, 2024, (9): 131-140.
[25]	毛立彦, 李慧敏, 龙凌云, 等. 基于SSR分子标记的睡莲遗传多样性分析 [J]. 南京林业大学学报: 自然科学版, 2024, 48(5), 57-68.
	Mao LY, Li HM, Long LY, et al. Analyzing the genetic diversity of Nymphaea spp. based on SSR markers [J]. Journal of Nanjing Forestry University Natural Sciences Edition, 2024, 48(5), 57-68.
[26]	梁燕, 韩传明, 孙超, 等. 基于SSR标记的核桃种质资源遗传多样性与遗传结构分析 [J]. 北方园艺, 2022(9): 47-54.
	Liang Y, Han CM, Sun C, et al. Genetic diversity and genetic structure analysis of walnut germplasm resources based on SSR markers [J]. North Hortic, 2022(9): 47-54.
[27]	Tiwari JK, Ali N, Devi S, et al. Analysis of allelic variation in wild potato (Solanum) species by simple sequence repeat (SSR) markers [J]. 3 Biotech, 2019, 9(7): 262.
[28]	Hu J, Mei M, Jin F, et al. Phenotypic variability and genetic diversity analysis of cultivated potatoes in China [J]. Front Plant Sci, 2022, 13: 954162.
[29]	王衍莉, 杨义明, 范书田, 等. 基于SSR分子标记的73份山葡萄及杂交后代的遗传多样性分析 [J].生物技术通报, 2021, 37(1): 189-197.
	Wang YL, Yang YM, Fan ST, et al. Genetic diversity analysis of 73 Vitis amurensis and its hybrids offsprings based on SSR molecular markers [J]. Biotechnology Bulletin, 2021, 37(1):189-197.
[30]	闫国跃, 杨帆, 白燕远, 等. 69份苦玄参种质SSR遗传多样性及品质性状相关标记分析 [J]. 中国实验方剂学杂志, 2020, 26(4): 174-184.
	Yan GY, Yang F, Bai YY, et al. SSR-based analysis of genetic diversity and quality trait-related markers of 69 Picria felterrae germplasm samples [J]. Chin J Exp Tradit Med Formulae, 2020, 26(4): 174-184.
[31]	赵成日. 中韩野生软枣猕猴桃种质资源遗传多样性分析 [J]. 果树学报, 2018, 35(9): 1043-1051.
	Zhao CR. The genetic diversity of wild Actinidia arguta germplasm resources from China and South Korea [J]. J Fruit Sci, 2018, 35(9): 1043-1051.
[32]	Wang Y, Rashid MAR, Li XP, et al. Collection and evaluation of genetic diversity and population structure of potato landraces and varieties in China [J]. Front Plant Sci, 2019, 10: 139.
[33]	刘有春, 刘成, 杨艳敏, 等. 基于EST-SSR标记的越橘栽培种和几个中国野生种的遗传结构分析 [J]. 果树学报, 2017, 34(8): 956-967.
	Liu YC, Liu C, Yang YM, et al. Genetic structure analysis of the cultivated blueberry (Vaccinium spp.) species and wild species in China based on EST-SSR markers [J]. J Fruit Sci, 2017, 34(8): 956-967.
[34]	王琰琰, 王俊, 刘国祥, 等. 基于SSR标记的雪茄烟种质资源指纹图谱库的构建及遗传多样性分析 [J]. 作物学报, 2021, 47(7): 1259-1274.
	Wang YY, Wang J, Liu GX, et al. Construction of SSR fingerprint database and genetic diversity analysis of cigar germplasm resources [J]. Acta Agron Sin, 2021, 47(7): 1259-1274.
[35]	张晓煜, 王仕鹏, 曹昆山, 等. 基于表型性状与SSR标记的马铃薯种质资源遗传多样性研究 [J]. 西北农业学报, 2024, 33(8):1436-1447.
	Zhang XY, Wang SP, Cao KS, et al. Genetic diversity of potato germplasm resources based on phenotypic traits and SSR markers [J]. China Ind Econ, 2024, 33(8):1436-1447.
[36]	孙泽硕, 蒋冬月, 柳新红, 等. 基于SSR标记的42份樱花品种的聚类分析及DNA指纹图谱构建 [J]. 园艺学报, 2023, 50(3): 657-668.
	Sun ZS, Jiang DY, Liu XH, et al. Cluster analysis and construction of DNA fingerprinting of 42 oriental cultivars of flowering cherry based on SSR markers [J]. Acta Hortic Sin, 2023, 50(3): 657-668.
[37]	李佩华, 张伦, 罗杰,等. 四川山区63份马铃薯亲本材料的SSR指纹图谱构建及遗传多样性分析 [J]. 四川农业大学学报, 2024, 42(4): 792-799.
	Li PH, Zhang L, Luo J, et al. Construction of SSR fingerprints and genetic diversity analysis of 63 potato materials in mountainous regions of sichuan province [J]. Journal of Sichuan Agricultural University, 2024,42(4):792-799.
[38]	王丹, 张志成, 韩海霞, 等. 基于SSR技术的马铃薯种质资源遗传多样性分析 [J]. 作物杂志, 2025, 4: 29-40.
	Wang D, Zhang ZC, Han HX, et al. Genetic diversity analysis of potato germplasm resources based on SSR technology [J]. Crops, 2025, 4: 29-40.
[39]	刘文林, 张举梅, 盛万民, 等. 52份俄罗斯引进马铃薯种质资源的遗传多样性与分子身份证构建 [J]. 分子植物育种, 2016, 14(1): 251-258.
	Liu WL, Zhang JM, Sheng WM, et al. Genetic diversity analysis and molecular identities establishment of 52 Russian potato varieties [J]. Mol Plant Breed, 2016, 14(1): 251-258.

编号 No.	引物名称 Name	引物序列 Primer sequence （5′-3′）	染色体 Chromosome	片段大小 Fragment （bp）	荧光 Tag
P1	PM0936	TGCACTTTCTTTGTCACCATTC TGACCTACTGTATGCTTTGGAA	Ⅸ	102-151	FAM
P2	PM0642	TCCATATGCAAGTGGCAAAA CACGTTGGATCCAGGAGGTA	Ⅹ	112-163	FAM
P3	PM0938	AGAAATTGGCAGAGCATTTAGCTG GGATTAGACAAACCTTCTTTTCCACA	Ⅲ	137-177	FAM
P4	PM0355	CCATCATGAGCCTTGCTTACATTT CAACAAGGCACAAAGCTAACAGAA	Ⅷ	172-235	FAM
P5	STM1052	CGTTAAGGAGGAGGAGGAAAA CCAAATAACGTGTTGAGCCC	Ⅳ	199-262	FAM
P6	STI032	TAGGGATGAGCTTCAACTCG CGGTTCTGACTTGGACTAATGA	Ⅶ	100-127	FAM
P7	STI033	CAATTTCGTTTTTTCATGTGACAC ATGGCGTAATTTGATTTAATACGTAA	Ⅸ	109-133	FAM
P8	STG0016	ATCCTTCTATGGCGTTCCAA ATTGCTCAAGAATGGCCAC	Ⅶ	116-156	FAM
P9	STM0019a	TGAGGGTTTTCAGAAAGGGA CATCCTTGCAACAACCTCCT	Ⅶ	133-233	FAM
P10	STM3012	TATGGAAATTCCGGTGATGG GACGGTGACAAAGAGGAAGG	Ⅺ	145-267	FAM
P11	STI0012	AATAGGTGTACTGACTCTCAATG TTGAAGTAAAAGTCCTAGTATGTG	Ⅵ	152-199	FAM
P12	STM2022	TGATTCTCTTGCCTACTGTAATCG CAAAGTGGTGTGAAGCTGTGA	Ⅷ	162-341	FAM
P13	STM1049	TCTCCCCATCTTAATGTTTC CAACACAGCATACAGATCATC	Ⅲ	168-197	FAM
P14	SSR0387	TTGATGAAAGGAATGCAGCTTGTG ACGTTAAAGAAGTGAGAGTACGAC	Ⅴ	141-187	FAM

编号 No.	引物名称 Name	引物序列 Primer sequence （5′-3′）	染色体 Chromosome	片段大小 Fragment （bp）	荧光 Tag
P1	PM0936	TGCACTTTCTTTGTCACCATTC TGACCTACTGTATGCTTTGGAA	Ⅸ	102-151	FAM
P2	PM0642	TCCATATGCAAGTGGCAAAA CACGTTGGATCCAGGAGGTA	Ⅹ	112-163	FAM
P3	PM0938	AGAAATTGGCAGAGCATTTAGCTG GGATTAGACAAACCTTCTTTTCCACA	Ⅲ	137-177	FAM
P4	PM0355	CCATCATGAGCCTTGCTTACATTT CAACAAGGCACAAAGCTAACAGAA	Ⅷ	172-235	FAM
P5	STM1052	CGTTAAGGAGGAGGAGGAAAA CCAAATAACGTGTTGAGCCC	Ⅳ	199-262	FAM
P6	STI032	TAGGGATGAGCTTCAACTCG CGGTTCTGACTTGGACTAATGA	Ⅶ	100-127	FAM
P7	STI033	CAATTTCGTTTTTTCATGTGACAC ATGGCGTAATTTGATTTAATACGTAA	Ⅸ	109-133	FAM
P8	STG0016	ATCCTTCTATGGCGTTCCAA ATTGCTCAAGAATGGCCAC	Ⅶ	116-156	FAM
P9	STM0019a	TGAGGGTTTTCAGAAAGGGA CATCCTTGCAACAACCTCCT	Ⅶ	133-233	FAM
P10	STM3012	TATGGAAATTCCGGTGATGG GACGGTGACAAAGAGGAAGG	Ⅺ	145-267	FAM
P11	STI0012	AATAGGTGTACTGACTCTCAATG TTGAAGTAAAAGTCCTAGTATGTG	Ⅵ	152-199	FAM
P12	STM2022	TGATTCTCTTGCCTACTGTAATCG CAAAGTGGTGTGAAGCTGTGA	Ⅷ	162-341	FAM
P13	STM1049	TCTCCCCATCTTAATGTTTC CAACACAGCATACAGATCATC	Ⅲ	168-197	FAM
P14	SSR0387	TTGATGAAAGGAATGCAGCTTGTG ACGTTAAAGAAGTGAGAGTACGAC	Ⅴ	141-187	FAM

序号 Code	引物 Primer	Na*	Ne*	H*	I*	多态位点 Polymorphic alleles	多态百分率 Percentage of polymorphic bands （%）	PIC
P1	PM0936	1.952 4	1.085 3	0.074 2	0.152 3	20	95.24	0.917 7
P2	PM0642	1.923 1	1.049 9	0.042 7	0.094 8	36	92.31	0.917 2
P3	PM0938	1.963	1.062 3	0.054 3	0.116 6	26	96.3	0.914 5
P4	PM0355	1.731 7	1.033 8	0.031	0.071 9	30	73.17	0.936 5
P5	STM1052	1.666 7	1.042 8	0.036 6	0.078 4	22	66.67	0.884 9
P6	STI032	1.8	1.159 7	0.114 8	0.200 8	8	80	0.758 5
P7	STI033	1.818 2	1.147 8	0.102	0.183	9	81.82	0.723 9
P8	STG0016	1.866 7	1.117 3	0.093 1	0.176 4	13	86.67	0.856 5
P9	STM0019a	1.808 5	1.033 7	0.030 3	0.071	38	80.85	0.931 9
P10	STM3012	1.889	1.061 8	0.041 4	0.082 7	24	88.89	0.683 2
P11	STI0012	2.0	1.089 9	0.070 2	0.135 3	16	100	0.809 9
P12	STM2022	1.575 8	1.075 9	0.054 8	0.102	19	57.58	0.850 2
P13	STM1049	1.916 7	1.128 9	0.096 5	0.174 9	11	91.67	0.787 1
P14	SSR0387	1.9	1.095 1	0.080 5	0.159 8	18	90	0.906 4
均值 Mean		1.843 7	1.084 6	0.065 9	0.128 6	20.71	84.37	0.848 4

序号 Code	引物 Primer	Na*	Ne*	H*	I*	多态位点 Polymorphic alleles	多态百分率 Percentage of polymorphic bands （%）	PIC
P1	PM0936	1.952 4	1.085 3	0.074 2	0.152 3	20	95.24	0.917 7
P2	PM0642	1.923 1	1.049 9	0.042 7	0.094 8	36	92.31	0.917 2
P3	PM0938	1.963	1.062 3	0.054 3	0.116 6	26	96.3	0.914 5
P4	PM0355	1.731 7	1.033 8	0.031	0.071 9	30	73.17	0.936 5
P5	STM1052	1.666 7	1.042 8	0.036 6	0.078 4	22	66.67	0.884 9
P6	STI032	1.8	1.159 7	0.114 8	0.200 8	8	80	0.758 5
P7	STI033	1.818 2	1.147 8	0.102	0.183	9	81.82	0.723 9
P8	STG0016	1.866 7	1.117 3	0.093 1	0.176 4	13	86.67	0.856 5
P9	STM0019a	1.808 5	1.033 7	0.030 3	0.071	38	80.85	0.931 9
P10	STM3012	1.889	1.061 8	0.041 4	0.082 7	24	88.89	0.683 2
P11	STI0012	2.0	1.089 9	0.070 2	0.135 3	16	100	0.809 9
P12	STM2022	1.575 8	1.075 9	0.054 8	0.102	19	57.58	0.850 2
P13	STM1049	1.916 7	1.128 9	0.096 5	0.174 9	11	91.67	0.787 1
P14	SSR0387	1.9	1.095 1	0.080 5	0.159 8	18	90	0.906 4
均值 Mean		1.843 7	1.084 6	0.065 9	0.128 6	20.71	84.37	0.848 4

编号 No.	CCCAP编号 GCD code	SSR指纹 SSR fingerprinting	编号 No.	CCCAP编号 GCD code	SSR指纹 SSR fingerprinting
1	CCCAP26	17C3639478	38	CCCAP2642	7HGI589
2	CCCAP50	178469JN34638E	39	CCCAP2643	84639BH78
3	CCCAP968	869988	40	CCCAP2644	2F369
4	CCCAP969	8JL988	41	CCCAP2645	368A34BD659
5	CCCAP970	8I563988	42	CCCAP2646	37A4D558
6	CCCAP971	8463778	43	CCCAP2647	KNO46DF8A1
7	CCCAP972	86988	44	CCCAP2648	123B671
8	CCCAP975	86396738	45	CCCAP2649	318G81
9	CCCAP976	584I3988	46	CCCAP2650	8E3K98
10	CCCAP977	84I9JN738	47	CCCAP2652	6963B77A
11	CCCAP978	84I9738	48	CCCAP2653	145I9H561
12	CCCAP979	8F69738	49	CCCAP2654	D5919H5E
13	CCCAP980	386397838	50	CCCAP2658	9A3686
14	CCCAP2615	69FGI3693	51	CCCAP2659	EJ7161
15	CCCAP2616	135469717	52	CCCAP2660	BC7E3J61
16	CCCAP2617	5L4Q39582	53	CCCAP2661	3E3J6A
17	CCCAP2618	8F469H88	54	CCCAP2663	8CF6E39JN683689
18	CCCAP2619	46D34569	55	CCCAP2664	8K53561
19	CCCAP2620	46CEJ568D	56	CCCAP2665	494D48
20	CCCAP2621	3CFJ56AC	57	CCCAP2668	H53837
21	CCCAP2623	3CJ34JK536	58	CCCAP2670	84I9H738
22	CCCAP2624	57FI55	59	CCCAP2671	7FG39838
23	CCCAP2626	BHI656	60	CCCAP2673	4679448
24	CCCAP2627	9FR1I61	61	CCCAP2674	46673
25	CCCAP2628	59CJ362	62	CCCAP2675	3853568
26	CCCAP2629	35CE3J568E	63	CCCAP2676	7A8969
27	CCCAP2630	9AB34JK566E	64	CCCAP2678	6A8962
28	CCCAP2631	66E982	65	CCCAP2679	C639468
29	CCCAP2632	697M3B61	66	CCCAP2680	6B6G39H687E
30	CCCAP2633	CK3739	67	CCCAP2681	7C5H3B66A
31	CCCAP2634	715361	68	CCCAP2682	7583B51
32	CCCAP2635	39JO59EH68	69	CCCAP2683	67G3B61
33	CCCAP2637	749H6A1	70	CCCAP2684	6A539BH626
34	CCCAP2638	4D639689	71	CCCAP2685	EF459EH88A
35	CCCAP2639	9B693961	72	CCCAP2686	3E623AB67
36	CCCAP2640	7CF13IJ658	73	CCCAP2687	3E623AB657
37	CCCAP2641	17I359B