基于非靶向代谢组解析红花石蒜叶片对高温胁迫的响应

doi:10.13560/j.cnki.biotech.bull.1985.2025-0874

摘要/Abstract

摘要：

目的揭示高温胁迫下石蒜属植物叶片的代谢响应特征及相关通路，为培育耐极端高温生境的石蒜属新种质提供理论依据。方法以红花石蒜为试验材料，于人工气候培养箱中进行42 ℃高温处理，并分别于处理0 h（对照）、6 h和12 h采集叶片样品，通过LC-MS技术进行非靶向代谢组学分析。结果在3个比较组（HS_6 h vs HS_0 h、HS_12 h vs HS_0 h和HS_12 h vs HS_6 h）中分别筛选出338、426、262种差异代谢物。在高温胁迫初期（42 ℃，6 h），差异代谢物活性整体上被显著抑制，而在高温胁迫后期（42 ℃，12 h）则转向以上调为主导的代谢重编程。进一步分析表明，脂类和氨基酸及其衍生物是高温胁迫的主要差异代谢物类别，其中脂类在胁迫初期以下调为主导，而氨基酸及其衍生物则在胁迫后期呈现显著上调积累。KEGG代谢通路富集分析显示，高温胁迫诱导的差异代谢物主要参与由“磷脂酰胆碱水解驱动”的脂质代谢途径（含亚油酸代谢、花生四烯酸代谢、α-亚麻酸代谢和甘油磷脂代谢4条支路）和以多种氨基酸（L-脯氨酸、L-苯丙氨酸等）显著积累为特征的氨基酸代谢途径（主要包括氨酰-tRNA生物合成、D-氨基酸代谢和苯丙氨酸代谢3条支路）及次生代谢物的生物合成。结论红花石蒜通过“脂质介导的持续膜稳态调控”结合“后期氨基酸驱动的防御网络强化”的协同策略，共同构建其高温适应性网络。

关键词: 红花石蒜, 高温胁迫, 代谢组学, 脂类代谢, 氨基酸代谢

Abstract:

Objective To decipher the metabolic response characteristics and associated pathways in Lycoris leaves under heat stress, and to provide a theoretical basis for cultivating new Lycoris germplasm that can tolerate extreme high-temperature habitats. Method Using Lycoris radiata as plant material, heat stress treatment was conducted at 42 ℃ in a controlled environment chamber. Leaf samples were collected at 0 h (control), 6 h, and 12 h after treatment initiation, followed by untargeted metabolomics profiling via LC-MS technology. Result Across three comparison groups (HS_6 h vs HS_0 h, HS_12 h vs HS_0 h, and HS_12 h vs HS_6 h), 338, 426, and 262 differential metabolites were identified, respectively. During early heat stress (42 ℃, 6 h), metabolic activity was significantly suppressed overall. Conversely, prolonged exposure (42 ℃, 12 h) triggered a metabolic reprogramming shift dominated by up-regulation. Further analysis revealed that lipids, amino acids and derivatives constituted the major classes of differential metabolites under heat stress. Notably, lipids were mainly down-regulated during the initial phase of heat stress, whereas amino acids and derivatives showed significant up-regulation and accumulation in the later stress stage. KEGG pathway enrichment analysis revealed that heat stress-induced differential metabolites were primarily enriched in: Lipid metabolic pathways driven by phosphatidylcholine hydrolysis (encompassing four sub-pathways: Linoleic acid metabolism, arachidonic acid metabolism, alpha-linolenic acid metabolism, and glycerophospholipid metabolism), amino acid metabolic pathways characterized by significant accumulation of multiple amino acids (e.g., L-proline, L-phenylalanine) (involving three core sub-pathways: Aminoacyl-tRNA biosynthesis, D-amino acid metabolism and phenylalanine metabolism) and biosynthesis of secondary metabolites. Conclusion L. radiata employs a synergistic strategy combining “lipid-mediated sustained regulation of membrane homeostasis” with “reinforcement of amino acid-driven defense networks in later stages” to collectively establish its thermal adaptation network.

Key words: Lycoris radiata, heat stress, metabolomics, lipid metabolism, amino acid metabolism

游欣, 王淅, 张新妤, 魏绪英, 程华, 蔡军火. 基于非靶向代谢组解析红花石蒜叶片对高温胁迫的响应[J]. 生物技术通报, 2026, 42(7): 173-181.

YOU Xin, WANG Xi, ZHANG Xin-yu, WEI Xu-ying, CHENG Hua, CAI Jun-huo. Analysis of Response of Lycoris radiata Leaves to Heat Stress Revealed by Untargeted Metabolomics[J]. Biotechnology Bulletin, 2026, 42(7): 173-181.

图/表 7

图1 红花石蒜叶片中鉴定出的所有代谢物类别

Fig. 1 All classification of metabolites identified in the leaves of Lycoris radiata

图2 主成分分析

Fig. 2 Principal component (PC) analysis

图3 OPLS-DA得分图A：HS_6 h vs HS_0 h；B：HS_12 h vs HS_0 h；C：HS_12 h vs HS_6 h

Fig. 3 OPLS-DA score diagram

图4 高温胁迫下红花石蒜叶片中差异代谢物火山图A：HS_6 h vs HS_0 h；B：HS_12 h vs HS_0 h；C：HS_12 h vs HS_6 h

Fig. 4 Volcano diagram of differential metabolites in the leaves of L. radiata under heat stress

图5 高温胁迫下红花石蒜叶片中差异代谢物的类别（A）及差异倍数前5代谢物分析（B）

Fig. 5 Categories of differential metabolites (A) and analysis of the top 5 differential metabolites by fold change (B) in the leaves of L. radiata under heat stress

图6 高温胁迫下红花石蒜叶片差异代谢物KEGG富集气泡图A：HS_6 h VS HS_0 h；B：HS_12 h VS HS_0 h；C：HS_12 h VS HS_6 h

Fig. 6 KEGG enrichment bubble diagram of differential metabolites in the leaves of L. radiata under heat stress

图7 高温胁迫下红花石蒜叶片脂质代谢（A）及氨基酸代谢（B）通路图

Fig. 7 Pathway diagram of lipid metabolism (A) and amino acid metabolism (B) in the leaves of L. radiata under heat stress

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