生物技术通报 ›› 2014, Vol. 0 ›› Issue (2): 107-111.

• 研究报告 • 上一篇    下一篇

家蝇泛素结合酶基因的生物信息学分析

国果, 吴沁怡, 吴建伟, 赵学军, 付萍, 张勇   

  1. 贵阳医学院 寄生虫学教研室,贵阳 550004
  • 收稿日期:2013-07-19 出版日期:2014-02-27 发布日期:2014-02-27
  • 作者简介:国果,女,博士,副教授,研究方向:昆虫分子免疫学;E-mail:guoguojsc@163.com
  • 基金资助:
    国家自然科学基金项目(81160204),贵州省科技厅基金[黔科合(2010)3160],高校博士点学科专项科研基金(20105215120001)

Bioinformatics Analysis of the Gene and Protein of Ubiquitin-conjugated Enzyme from Musca domestic

Guo Guo, Wu Qinyi, Wu Jianwei, Zhao Xuejun, Fu Ping, Zhang Yong   

  1. Department of ParasitologyGuiyan Medical CollegeGuiyang 550004)
  • Received:2013-07-19 Published:2014-02-27 Online:2014-02-27

摘要: 利用EST测序技术从家蝇幼虫cDNA文库中获得家蝇泛素结合酶基因(MD-E2)cDNA序列,采用NCBI、ExPASY中的相关工具,对该基因及其编码蛋白的基本理化性质、疏水性、信号肽、二级结构和亚细胞定位等方面进行预测和分析,MEGA软件构建了进化树。结果表明,家蝇泛素结合酶基因的cDNA序列具有完整的ORF框,全长480 bp,一共编码159个氨基酸。其编码的蛋白质理论分子量为18.117 9 kD,等电点8.64,无信号肽及跨膜区,属于亲水性蛋白,具有泛素结合酶家族的活性位点(FHPNVYPSGTVCLSLL),主要分布于细胞核;二级结构以无规则卷曲为主,β折叠较少。

关键词: 家蝇, 泛素结合酶, 生物信息学

Abstract: A cDNA sequence of Musca domestic ubiquitin-conjugated enzyme(MD-E2)was obtained by in EST technology. Some characters of the MD-E2 gene and encoded protein sequence were predicted and analyzed by bioinformatics methods in the following aspects, such as general physical and chemical properties, hydrophobicity, signal peptide, secondary structure and localization sites in cells. Results showed that the MD-E2 contains a complete ORF(480bp)encoding 159 amino acid with a predicted molecular weight about 18.117 9 kD and pI 8.64. The protein encoded by MD-E2 gene has no signal peptide, and no transmembrane domains, and it is a hydrophilic protein which is mainly located in cell nucleus. The secondary structures are mainly composed of random coil.