香蕉MaBTB基因的表达分析及亚细胞定位

生物技术通报 ›› 2014, Vol. 0 ›› Issue (4): 71-76.

香蕉MaBTB基因的表达分析及亚细胞定位

柴文龙¹, 金志强³, 贾彩红², 刘菊华², 苗红霞², 张建斌², 徐碧玉²

(1.海南大学农学院，海口 570228；
2.中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室，海口 571101；
3.中国热带农业科学院海口实验站海南省香蕉遗传改良重点实验室，海口 570102)

收稿日期:2013-09-23 出版日期:2014-04-29 发布日期:2014-04-29
作者简介:柴文龙，男，硕士研究生，研究方向：作物遗传育种；E-mail：chaiwl2012@163.com
基金资助:
中央级公益性科研院所基本科研业务专项(ITBB110216)，海南省重大科技项目子课题(ZDZX2013023-1)

Expression Analysis and Subcellular Localization of the MaBTB Gene in Banana

Chai Wenlong¹, Jin Zhiqiang³, Jia Caihong², Liu Juhua², Miao Hongxia², Zhang Jianbin², Xu Biyu²,

(1. Department of Agriculture，Hainan University，Haikou 570228；
2. Key Laboratory of Biology and Genetic Resources of Tropical Crops， Ministry of Agriculture，Institute of Tropical Bioscience and Biotechnology，Chinese Academy of Tropical Agricultural Sciences，Haikou 571101； 3. Haikou Experimental Station，Institute of Banana，Chinese Academy of Tropical Agricultural Sciences，Haikou 570102)

Received:2013-09-23 Published:2014-04-29 Online:2014-04-29

摘要/Abstract

摘要： 利用荧光定量PCR分析在不同处理下的香蕉采后果实中MaBTB基因的表达，在正常成熟的果实中，MaBTB基因的表达与乙烯的释放量呈正相关；相反，在1-MCP处理的香蕉果实中，该基因的表达相对变化不明显；用乙烯处理的香蕉果实，其表达量在第3天达到高峰，比正常成熟的早11 d。这些结果表明MaBTB基因在香蕉采后果实中是受乙烯诱导表达的。最后，亚细胞定位分析表明MaBTB基因定位在细胞核上。

关键词: 香蕉, MaBTB基因, 表达分析, 亚细胞定位

Abstract: Real-time quantitative PCR of postharvest banana revealed that MaBTB exhibited differential expression patterns that are associated with ethylene biosynthesis. In naturally ripened bananas, expression of MaBTB was in accordance with ethylene biosynthesis. In contrast, for 1-methylcyclopropene-treated bananas, MaBTB expression levels remained constant. Following treatment with ethylene, MaBTB expression in banana fruit significantly increased with ethylene biosynthesis and peaked 3 d after harvest, which was 11 d earlier than that for naturally ripened banana fruits. These results suggested that MaBTB expression was induced by ethylene for regulation of postharvest banana ripening. Finally, subcellular localization assays showed that the MaBTB protein localizes to the nucleus.

Key words: Banana(Musa acuminataL. AAA group), MaBTB gene, Expression analysis, Subcelluar localization

柴文龙, 金志强, 贾彩红, 刘菊华, 苗红霞, 张建斌, 徐碧玉. 香蕉MaBTB基因的表达分析及亚细胞定位[J]. 生物技术通报, 2014, 0(4): 71-76.

Chai Wenlong, Jin Zhiqiang, Jia Caihong, Liu Juhua, Miao Hongxia, Zhang Jianbin, Xu Biyu,. Expression Analysis and Subcellular Localization of the MaBTB Gene in Banana[J]. Biotechnology Bulletin, 2014, 0(4): 71-76.

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