贵州三个地方猪种的系统发育分析

生物技术通报 ›› 2014, Vol. 0 ›› Issue (4): 96-101.

贵州三个地方猪种的系统发育分析

丁玫^1，2, 陈祥^1，2, 许厚强¹, 吴通贵³, 冯文武¹, 柏琳¹

(1.贵州大学动物科学学院高原山地动物遗传育种与繁殖省部共建教育部重点实验室，贵阳 550025；
2.贵州省生猪健康养殖工程技术研究中心，贵阳 550025；
3. 施秉县畜禽品种改良站，凯里 556200)

收稿日期:2013-12-20 出版日期:2014-04-29 发布日期:2014-04-29
作者简介:丁玫，女，研究生，硕士研究生，研究方向：动物遗传育种与繁殖；E-mail：ddingtang@yeah.net
基金资助:
贵州省动植物育种专项(黔农育专字［2011]019号)，贵州省农业公关项目(黔科合NY［2013]3071号)，贵州省工程中心建设项目(黔科合农C字［2001]4022号)

Phylogenetic Analysis of Three Domestic Pig Breeds in Guizhou Province

Ding Mei¹, Chen Xiang^1，2, Xu Houqiang¹, Wu Tonggui³, Feng Wenwu¹, Bai Lin¹,

(1.Key Laboratory of Animal Genetics，Breeding and Reproduction in the Plateau Mountainous Region，Ministry of Education，College of Animal Science，Guizhou University，Guiyang 550025；
2.Guizhou Engineering Technology Research Center for Healthy Pig Breeding，Guiyang 550025；
3.Shibing Breed Improvement Centre for Livestock and Poultry，Kaili 556200)

Received:2013-12-20 Published:2014-04-29 Online:2014-04-29

摘要/Abstract

摘要： 采用PCR产物直接测序方法首次测定贵州3个地方猪种(白洗猪、从江香猪、黔北黑猪)共计57个个体线粒体DNA控制区的全序列，将它们与GenBank上已知的25个国内外猪种一起计算遗传距离，并构建系统发育树。结果表明，3个贵州地方猪种mtDNA D-loop区的序列长度为1 254-1 314 bp之间，贵州地方猪种遗传距离在0.002 9-0.007 2之间，白洗猪与黔北黑猪、从江香猪之间的距离最大，均为0.007 2。系统发育分析的结果支持中国家猪分别有南北两个母系起源的观点，且各地方猪种之间可能有基因交流。

关键词: mt, DNA, D-loop, 遗传距离, 地方猪种, 系统发育

Abstract: PCR product of the complete mitochondrial control regions of 57 pigs from 3 Chinese pig breeds in Guizhou(Baixi pig, Congjiangxiang pig and Qianbeihei pig)were directly sequenced, the genetic distance between the mtDNA of three breeds of pig with other 25 pig breeds was calculated, and the phylogenetic tree was built. The results revealed that, the sequences of the mtDNA D-loop of the three pig breeds were 1 254-1 314 bp, the genetic distance between the three breeds was 0.002 9-0.007 2, comparing Baixi pig with Qianbei Black pig and Congjiang xiang pig, the genetic distance of Baixi pig was great, all of 0.007 2. The results of phylogenetic anslysis supported the view that the Chinese pig breeds respectively originated from the Southern and the Northern, and there were gentic exchanging among the Chinses pig breeds.

Key words: mt, DNA, D-loop, Genetic distance, Domestic pig breeds, Phylogeny

丁玫, 陈祥, 许厚强, 吴通贵, 冯文武, 柏琳. 贵州三个地方猪种的系统发育分析[J]. 生物技术通报, 2014, 0(4): 96-101.

Ding Mei, Chen Xiang, Xu Houqiang, Wu Tonggui, Feng Wenwu, Bai Lin,. Phylogenetic Analysis of Three Domestic Pig Breeds in Guizhou Province[J]. Biotechnology Bulletin, 2014, 0(4): 96-101.

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