合作猪SLA-DQA基因外显子2多态性分析

生物技术通报 ›› 2014, Vol. 0 ›› Issue (4): 91-95.

合作猪SLA-DQA基因外显子2多态性分析

张国华¹, 马小军^1，2, 吕伟丽¹

(1.甘肃农业大学动物医学院，兰州 730037；
2. 甘肃省草食动物生物技术重点实验室，兰州 730070)

收稿日期:2013-10-30 出版日期:2014-04-29 发布日期:2014-04-29
作者简介:张国华，男，硕士研究生，研究方向：动物免疫与抗病；E-mail：664802394@qq.com
基金资助:
甘肃省自然科学基金项目(096RJZA005)

Analyzing Polymorphism of SLA-DQA Gene Exon 2 in the Hezuopig

Zhang Guohua¹, Ma Xiaojun^1，2, Lü Weili¹,

(1. College of Veterinary Medicine，Gansu Agricultural University，Lanzhou 730037；
2. Gansu Key Laboratory of Herbivorpous Animal Biotechnology，Lanzhou 730070)

Received:2013-10-30 Published:2014-04-29 Online:2014-04-29

摘要/Abstract

摘要： 旨在研究合作猪DQA基因外显子2多态性，确定其等位基因数、核苷酸多态位点、氨基酸多态位点及各个等位基因之间的遗传关系，分析其进化意义。选用PCR-SSCP对439只合作猪SLA-DQA基因外显子2的多态性进行检测；测序群体内因变异而产生的各等位基因序列，并分析序列数据。结果显示，在合作猪SLA-DQA外显子2中发现了7个新等位基因，共18个核苷酸多态位点，10个氨基酸多态位点。合作猪SLA-DQA外显子2具有较丰富的多态性，群体内可能蕴藏着更加丰富的遗传资源；合作猪SLA-DQA外显子2基因最初可能由一个等位基因突变分化成一大类基因；合作猪SLA-DQA外显子2序列与各个猪种的SLA-DQA外显子2序列具有较高的同源性，预示着这些猪种的SLA-DQA外显子2基因最早可能来源于其分歧之前的共同祖先原始序列；新发现的7个SLA-DQA外显子2等位基因，可能由遗传关系较近的两个等位基因突变产生。

关键词: 合作猪, SLA-DQA外显子2, 多态性, PCR-SSCP

Abstract: The polymorphism, allele number, nucleotide polymorphism sites, amino acid polymorphism sites, the genetic relationship and evolutionary significance of the alleles of the SLA-DQA gene exon 2 in Hezuo pigs were studied. Variation of SLA-DQA gene in the 439 Hezuopigwas investigated by amplification of exon 2 using PCR, followed by single-strand conformational polymorphism(SSCP), DNA sequencing and sequence data analysis. Result showed that seven alleles were found in the SLA-DQA geneexon 2. Eighteen nucleotide polymorphism sites and ten amino acid polymorphism sites were identified in seven SLA-DQA gene exon 2 haplotypes. The study indicated that a high level of sequence polymorphism existed in SLA-DQA gene exon 2 of Hezuo pigs, and the population might have more genetic resources. SLA-DQA gene exon 2 in Hezuo pigs might be differentiated into two major categories alleles from one mutant alleles at first. SLA-DQA gene exon 2 in Hezuo pigs and other pig had high homology, which indicated that SLA-DQA gene exon 2 in pig might come from primordial sequences that were present in a common ancestor and persisted in the pig populations since their divergence. Seven new alleles that had near genetic relationship, may came from the two near allele by mutation.

Key words: Hezuopig, SLA-DQA gene exon 2, Polymorphism, PCR-SSCP

张国华, 马小军, 吕伟丽. 合作猪SLA-DQA基因外显子2多态性分析[J]. 生物技术通报, 2014, 0(4): 91-95.

Zhang Guohua, Ma Xiaojun, Lü Weili,. Analyzing Polymorphism of SLA-DQA Gene Exon 2 in the Hezuopig[J]. Biotechnology Bulletin, 2014, 0(4): 91-95.

参考文献

[1] Snibson KJ, Maddox JF, Fabb SA, Brandon MR. Allelie variation of ovine MHC class II DQA1 and DQA2 genes[J]. Animal Genetics, 1998, 29：356-362.
[2] 孙东晓, 张沅, 李宁.蒙古绵羊和哈萨克绵羊MHC-DRB3基因外显子2的多态性[J].遗传学报, 2003, 30(8)：761-765.
[3] Janeway CA, Travers P, Walport M, Capra JD. Immunobiology：the immune system in health and disease［M]. 4th ed. London：Current Biology Publication, 1999.
[4] Raadsma HW. Fleece rot and boby strike in Merino Sheep V heritability of liability to boby strike in weaner sheep under fly wave conditions[J]. Australian Journal of Agricultural Research, 1991, 42：279-293.
[5] Ballinggall KT, Fardoe K, McKeever DJ. Genomic organization and allelic diversity within coding and non-coding regions of the Ovar-DRB1 locus[J]. Immunogenetics, 2008, 60：95-103.
[6] Rothschild MF. Selection for disease resistance in pig[J]. Pig News Inf, 1985, 6：277-280.
[7] 张冬杰. SLA-DQA 基因的多态性与仔猪初生重和30日龄体重的相关性分析[J].黑龙江农业科学, 2010, 1(1)：1-2.
[8] 谈永松, 王林云.猪MHCⅡ类区域基因研究进展[J].国外畜牧学—猪与禽, 2003, 23(1)：37-41.
[9] 彭勇波, 李奎, 樊斌等.猪SLA-DQA一个新SNP的发现及遗传效应的研究[J].中国农业科学, 2005, 38(12)：2526-2530.
[10] Amills M, Ramiya V, Norimine J, Lewin HA. The major histocompatibility complex of ruminants[J]. Rev Sci Tech, 1998, 17：108-120.
[11] 成述儒, 罗玉柱, 等.藏绵羊DQA1基因多态性分析[J].中国农业科学, 2011, 44(10)：2146-2153.
[12] 潘星华. MHC基因分子进化研究进展[J].国外医学：遗传学分册, 1995, 18(1)：7-11.
[13] 赵德荣, 孙岚玲.哺乳类MHC多态性的形成[J].国外医学：遗传学分册, 1995, 18(6)：330-332.
[14] 刘云芳, 王新峰, 等.多浪样MHC-DEB3基因座的PCR-RFLP多态性分析[J].遗传, 2004, 26(1)：59-62.
[15] Nagaoka Y, Kabeya H, Onuma M, et al. Ovine MHC class II DRB1 alleles associated with resistance or susceptibilitytodevelopment of bovine leukemia virus-induced ovine lymphoma[J]. Cancer Research, 1999, 59(4)：975-981.
[16] Konnai S, Takeshima SN, Tajima S, et al. The influence of ovine MHC class II DRB1 alleles onimmune response in bovine leukemia virus infection[J]. Microbiology and Immunology, 2003, 47(3)：223-232.
[17] 晏光荣, 马海明.分子标记在动物抗病育种中的研究进展[J].国外畜牧科技, 2001, 28(6)：32-34.
[18] 高树新, 许尚忠, 李金泉, 等. BoLA-DQA、DRB₃～*exon2多态性及其与奶牛乳房炎的关联分析[J].畜牧兽医学报, 2006, 37：317-320.
[19] 姚金梅, 连林生.猪SLAⅡ类基因多态性及其与抗病性状关联的研究进展[J].中国畜牧兽医, 2008, 35(10)：64-66.
[20] Kanai TH, Tanioka Y, Tanigawa M, et al. Allelic diversity at class DRB and DQB loci of the pig MHC[J]. Immunogenetics, 1999, 50(516)：295-300.