生物技术通报 ›› 2014, Vol. 0 ›› Issue (12): 97-104.doi: 10.13560/j.cnki.biotech.bull.1985.2014.12.016

• 研究报告 • 上一篇    下一篇

响应面法优化回流提取紫薯花青素工艺

刘倩, 齐计英, 韩静, 万阅 ,郑士彬   

  1. 沈阳药科大学制药工程学院,沈阳 110016
  • 收稿日期:2014-05-12 出版日期:2014-12-08 发布日期:2014-12-12
  • 作者简介:刘倩,女,硕士,研究方向:天然活性物质的提取与制剂研究;E-mail:18604019826@163.com

Optimization of Refluxing Extraction Technology of Anthocyanins from Purple Sweet Potato by Response Surface Method

Liu Qian, Qi Jiying, Han Jing, Wan Yue, Zheng Shibin   

  1. (School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016)
  • Received:2014-05-12 Published:2014-12-08 Online:2014-12-12

摘要: 旨在获得紫薯花青素的最佳提取工艺,采用中心组合(Box-Behnken)试验设计及响应面分析相结合的方法,对紫 薯花青素的回流提取工艺进行优化。单因素试验结果显示,最佳乙醇体积浓度为 75%、提取温度为 60℃、提取时间为 60 min、液 料比为 7∶1,在此基础上,利用中心组合(Box-Behnken)试验设计及响应面分析法进行回归分析,获得最优工艺参数为 :乙醇体 积浓度 74%、提取温度 66℃、提取时间 56 min、液料比 8∶1。在优化后的条件下进行验证试验,得到紫薯花青素的提取率为 4.64 mg/g,与理论值(4.68 mg/g)相比,相对误差较小,为 0.85%。研究表明,采用响应面法分析优化紫薯花青素的水浴回流提取工艺 参数准确可靠,可应用于紫薯花青素的提取生产。

关键词: 紫薯, 花青素, 回流提取, 中心组合设计, 响应面分析

Abstract: Response surface methodology(RSM)was used to optimize the refluxing extraction process of anthocyanins from purple sweet potatoes as a result of getting the optimum technology. The results of single-factor test showed that the best ethanol concentration was 70%, extraction temperature was 60℃, extraction time was 60 min, liquid-material ratio was 7∶1. Based on these results, the significant parameters were further optimized using Box-Behnken design, RSM and regression analysis. The optimum parameters were :ethanol concentration 74%, extraction temperature 66℃, extraction time 56 min, liquid-material ratio 8∶1. Under such conditions, the extraction efficiency could reach 4.64 mg/g, and have a smaller relative error at 0.85% compared with the theoretical value(4.68 mg/g). The study showed that the processing parameters achieved from these experiments were accurate and can be applied to the extraction and production of anthocyanins from purple sweet potatoes.

Key words: Purple sweet potatoes, Anthocyanin, Refluxing extraction central, Composite design, Response surface methodology