生物技术与我国猕猴桃育种

doi:10.13560/j.cnki.biotech.bull.1985.2015.07.036

摘要/Abstract

摘要： 猕猴桃是原产我国的重要果树之一。随着现代生物技术的发展，以野生和实生选优为主的传统猕猴桃育种方法与以基因组学和转基因为核心的分子育种技术相比，挑战和机遇并存。与其他果树相比，猕猴桃生物技术及其转基因研究较为滞后。就现代生物技术在猕猴桃遗传育种中的方法、应用及研究进展进行了综述，并对我国猕猴桃育种现状进行浅析，旨在为我国猕猴桃育种和产业发展提供方法参考及思路借鉴。

关键词: 猕猴桃, 基因组, 转基因, 育种

Abstract: Kiwifruit(Actinidia Lindl.)is an important fruit tree crop in China. With the development of modern biotechnology，traditional breeding of kiwifruit is mainly based on the selection from wilding and seedling，these methods presents coexist situation the challenge and opportunity compared with the application of molecular breeding such as the genomics and transgenesis. Compared with the other fruit trees，the researches for the genomics of kiwifruit are still at a relatively low level. This paper summarized the method，application，and the latest research progress of modern biotechnology in kiwifruit genetic breeding，and discussed the breeding strategy of kiwifruit in China，in order to provide the method and thought reference for kiwifruit conversional and transgenesis breeding in China.

Key words: Actinidia, genome, transgene, breeding

井赵斌, 雷玉山, 李永武. 生物技术与我国猕猴桃育种[J]. 生物技术通报, 2015, 31(7): 1-10.

Jing Zhaobin, Lei Yushan, Li Yongwu. Biotechnology and Kiwifruit Breeding in China[J]. Biotechnology Bulletin, 2015, 31(7): 1-10.

参考文献

[1] 黄宏文. 猕猴桃属分类资源驯化栽培[M]. 北京：科学出版社, 2013.
[2] Li X, Li J, Doejarto DD. Advances in the study of the systematics of Actinidia Lindley[J]. Frontiers of Biology in China, 2009, 4(1)：55-61.
[3] Crowhurst RN, Gleave AP, MacRae EA, et al. Analysis of expressed sequence tags from Actinidia：applications of a cross species EST database for gene discovery in the areas of flavor, health, color and ripening[J]. BMC Genomics, 2008, 9：351.
[4] Zhou J, Liu YF, Huang HW. Characterization of 15 Novel single nucleotide polymorphisms(Snps)in the Actinidia chinensis SPECIES COMplex(Actinidiaceae)[J]. American Journal of Botany, 2011, 98(5)：E100-E102.
[5] Crowhurst RN, Lints R, Atkinson RG, et al. Restriction fragment length polymorphisms in the genus Actinich'a(Actinidiaceae)[J]. Plant Systemtics and Evolution, 1990, 172：193-203.
[6] Huang WG, Cipriani G, Morgante M, et al. Microsatellite DNA in Actinidia chinensis：isolation, characterisation, and homology in related species[J]. Theoretical and Applied Genetics, 1998, 97(8)：1269-1278.
[7] Gill GP, Harvey CF, Gardner RC, et al. Development of sex-linked PCR markers for gender identification in Actinidia[J]. Theoretical and Applied Genetics, 1998, 97(3)：439-445.
[8] Xiao XG, Zhang LS, Li SH, et al. First step in the search for AFLP markers linked to sex in Actinidia[J]. Fourth International Symposium on Kiwifruit, Proceedings, 1999, 498：99-104.
[9] Testolin R, Huang WG, Lain O, et al. A kiwifruit(Actinidia spp.)linkage map based on microsatellites and integrated with AFLP markers[J]. Theoretical and Applied Genetics, 2001, 103(1)：30-36.
[10] Huang HW, Li ZZ, Li JQ, et al. Phylogenetic relationships in Actinidia as revealed by RAPD analysis[J]. Journal of the American Society for Horticultural Science, 2002, 127(5)：759-766.
[11] Shirkot P, Sharma DR, Mohapatra T. Molecular identification of sex in Actinidia deliciosa var. deliciosa by RAPD markers[J]. Scientia Horticulturae, 2002, 94(1-2)：33-39.
[12] Fraser LG, Harvey CF, Crowhurst RN, et al. EST-derived microsatellites from Actinidia species and their potential for mapping[J]. Theoretical and Applied Genetics, 2004, 108(6)：1010-1016.
[13] Korkovelos AE, Mavromatis AG, Huang WG, et al. Effectiveness of SSR molecular markers in evaluating the phylogenetic relationships among eight Actinidia species[J]. Scientia Horticulturae, 2008, 116(3)：305-310.
[14] Novo M, Romo S, Rey M, et al. Identification and sequence characterisation of molecular markers polymorphic between male kiwifruit(Actinidia chinensis var. deliciosa(A. Chev. )A. Chev.)accessions exhibiting different flowering time[J]. Euphytica, 2010, 175(1)：109-121.
[15] Zhen Y, Li Z, Huang HW, et al. Molecular characterization of kiwifruit(Actinidia)cultivars and selections using SSR markers[J]. Journal of the American Society of Horticultural Science, 2004, 129：374-382.
[16] 井赵斌, 俞靓, 魏琳, 等. 国外牧草基因组学和转基因技术研究进展[J]. 生物技术通报, 2011(11)：12-25.
[17] Palombi AM, Damiano C. Comparison between RAPD and SSR molecular markers in detecting variation in kiwifruit(Actinidia deliciosa A. Chev)[J]. Plant Cell Reports, 2002, 20：1061-1066.
[18] Korkovelos AE, Goulas CK, Vasilakakis MD. Screening microsatellites for their effectiveness to identify and differentiate among Actinidia genotypes[J]. Acta Hort. (ISHS), 2003, 610：357-363.
[19] Mavromatis AG, Arvanitoyannis I, Nanos G, et al. Molecular fingerprinting of a new kiwifruit cultivar(cv. Tsehelidis)and comparative analysis with cv. Hayward according to physicochemical properties[J]. Scientia Horticulturae, 2010, 125：277-282.
[20] Fraser LG, Tsang GK, Datson PM, et al. A gene-rich linkage map in the dioecious species Actinidia chinensis(kiwifruit)reveals putative X/Y sex-determining chromosomes[J]. BMC Genomics, 2009, 10：102.
[21] 汤佳乐, 吴寒, 郎彬彬, 等. 野生毛花猕猴桃叶片和果实AsA含量的SSR标记关联分析[J]. 园艺学报, 2014, 41(5)：833-840.
[22] Richardson AC, Boldingh HL, McAtee PA, et al. Fruit development of the diploid kiwifruit, Actindia chinensis 'Hort16A'. [J]BMC Plant Biology, 2011, 11：182.
[23] Varkonyi-Gasic E, Moss SM, Voogd C, et al. Identification and characterization of flowering genes in kiwifruit：sequence conserv-ation and role in kiwifruit flower development[J]. BMC Plant Biology, 2011, 11：72.
[24] 黄春辉, 高洁, 曲雪艳, 等. 中华猕猴桃黄肉色泽变化研究与转录组测序[J]. 园艺学报, 2012, 39：2607.
[25] Huang SX, Ding J, Deng DJ, et al. Deaft genome of the kiwifruit Actinidia chinensis[J]. Nature Communications, 2013, 4：2640.
[26] Harada H. In vitro organ culture of Actinidia chinensis Pl. as a technique for vegetative multiplication[J]. Journal of Horticultural Science, 1975, 50(1)：81-83.
[27] Lin QL, Chen ZQ, Wu JS. Propagation in vitro of some excellent clones of kiwifruit[J]. Journal of Fujian Agricultural University, 1994, 23(3)：271-274.
[28] Kumar S, Sharma DR. In vitro propagation of kiwifruit[J]. Journal of Horticultural Science and Biotechnology, 2002, 77(5)：503-508.
[29] Kim M, Kim SC, Moon DY, et al. Rapid shoot propagation from microcross sections of kiwifruit(Actinidia deliciosa cv. 'Hayward')[J]. Journal of Plant Biology, 2007, 50(6)：681-686.
[30] Barbieri C, Morini S. Plant regeneration from Actinidia callus cultures[J]. Journal of Horticultural Science, 1987, 62(1)：107-109.
[31] Xiao Z, Han B. Interspecific somatic hybrids in Actinidia[J]. Acta Botanica Sinica, 1997, 39(12)：1110-1117.
[32] Zhang YJ, Qian YQ, Mu XJ, et al. Plant regeneration from in vitro-cultured seedling leaf protoplasts of Actinidia eriantha Benth[J]. Plant Cell Reports, 1998, 17(10)：819-821.
[33] Xiao Z, Wan L, Han B. An interspecific somatic hybrid between Actinidia chinensis and Actinidia kolomikta and its chilling tolerance[J]. Plant Cell, Tissue and Organ Culture, 2004, 79(3)：299-306.
[34] 陈绪中, 李丽, 张忠慧, 等. 猕猴桃种间远缘杂交胚珠培养[J]. 果树学报, 2006, 23(4)：620-622.
[35] Mu SK, Fraser LG, Harvey CF. Initiation of callus and regeneration of plantlets from endosperm of Actinidia interspecific hybrids[J]. Scientia Horticulturae, 1990a, 44(1-2)：107-117.
[36] Hirsch AM, Testolin R, Brown S, et al. Embryo rescue from interspecific crosses in the genus Actinidia(kiwifruit)[J]. Plant Cell Reports, 2001, 20：508-516.
[37] Wu JH, Ferguson AR, Murray BG. Manipulation of ploidy for kiwifruit breeding：in vitro chromosome doubling in diploid Actinidia chinensis Planch[J]. Plant Cell Tissue Organ Culture, 2011, 106：503-511.
[38] Mu XJ, Wang WL, Cai DR, et al. Embryology and embryo rescue of an interspecific cross between Actinidia deliciosa cv. Hayward and A. eriantha[J]. Acta Botanica Sinica, 1990b, 32：425-431.
[39] 徐小彪, 辜青青, 蔡祖国, 等. 玻璃化法超低温保存猕猴桃离体茎尖及其植株再生[J]. 园艺学报, 2006, 33(4)：842-844.
[40] Bachiri Y, Song GQ, Plessis P, et al. Routine cryopreservation of kiwifruit(Actinidia spp)germplasm by encapsulation-dehydration：importance of plant growth regulators[J]. Cryo Letters, 2001, 22(1)：61-74.
[41] Wu Y, Zhao Y, Engelmann F, et al. Cryopreservation of kiwi shoot tips[J]. Cryo Letters, 2001, 22(5)：277-284.
[42] Zhai Z, Wu Y, Engelmann F, et al. Genetic stability assessments of plantlets regenerated from cryopreserved in vitro cultured grape and kiwi shoot-tips using RAPD[J]. Cryo Letters, 2003, 24(5)：315-322.
[43] Matsuta N, Iketani H, Hayashi T. Effect of acetosyringone on kiwifruit transformation[J]. Japan Journal of Breed, 1990, 40：184-185.
[44] Nakamura Y, Sawada H, Kobayashi S, et al. Expression of soybean beta -1, 3-endoglucanase cDNA and effect on disease tolerance in kiwifruit plants[J]. Plant Cell Reports, 1999, 18(7/8)：527-532.
[45] Kusaba S, Kano-Murakami Y, Matsuoka M, et al. Expression of the rice homeobox gene, OSH1, causes morphological changes in transgenic kiwifruit[J]. Journal of the Japanese Society for Horticultural Science, 1999, 68(3)：482-486.
[46] Kobayashi S, Ding CK, Nakamura Y, et al. Kiwifruits(Actinidia deliciosa)transformed with a Vitis stilbene synthase gene produce piceid(resveratrol-glucoside)[J]. Plant Cell Reports, 2000, 19(9)：904-910.
[47] Kim M, Kim S, Song K, et al. Transformation of carotenoid biosynthetic genes using a micro-cross section method in kiwifruit(Actinidia deliciosa cv. Hayward)[J]. Plant Cell Reports, 2010, 29(12)：1339-1349.
[48] Tian N, Wang J, Xu ZQ. Overexpression of Na⁺/H⁺ antiporter gene AtNHX1 from Arabidopsis thaliana improves the salt tolerance of kiwifruit(Actinidia deliciosa)[J]. South African Journal of Botany, 2011, 77(1)：160-169.
[49] Uematsu C, Murase M, Ichikawa H, et al. Agrobacterium-mediated transformation and regeneration of kiwifruit[J]. Plant Cell Reports, 1991, 10(6/7)：286-290.
[50] Famiani F, Ferradini N, Standardi A, et al. In vitro regeneration of different Actinidia species[J]. Acta Horticulturae, 1997, 444：133-138.
[51] Han M, Gleave AP, Wang T. Efficient transformation of Actinidia arguta by reducing the strength of basal salts in the medium to alleviate callus browning[J]. Plant Biotechnology Reports, 2010, 4(2)：129-138.
[52] Wang T, Atkinson R, Janssen B. The choice of Agrobacterium strain for transformation of kiwifruit[J]. Acta Horticulturae, 2007, 753(1)：227-232.
[53] HortResearch. Kiwifruit Genomics Programme. HortResearch, Auckland, 2005.
[54] Fraser LG, Kent J, Harvey CF. Transformation studies of Actinidia chinensis Planch[J]. New Zealand Journal of Crop Horticulture Science, 1995, 23：407-413.
[55] Wang T, Atkinson R, Janssen B. The choice of Agrobacterium strain for transformation of kiwifruit[J]. Acta Horticulturae, 2007, 753(1)：227-232.
[56] Oliveira MM, Barroso J, Pais MS. Direct gene transfer into Actinidia deliciosa protoplasts：analysis of transient expression of the CAT gene using TLC autoradiography and a GC-MS based method[J]. Plant Molecular Biology, 1991, 17：235-242.
[57] Janssen BJ, Gardner RC. The use of transient GUS expression to develop an Agrobacterium-mediated gene-transfer system for kiwifruit[J]. Plant Cell Reports, 1993, 13：28-31.
[58] Yazawa M, Suginuma C, Ichikawa K, et al. Regeneration of transgenic plants fromhairy root of kiwi fruit(Actinidia deliciosa)induced by Agrobacterium rhizogenes[J]. Breed Science, 1995, 45：241-244.
[59] Yamakawa Y, Chen LH. Agrobacterium rhizogenes-mediated transformation of kiwifruit(Actinidia deliciosa)by direct formation of adventitious buds[J]. Journal of the Japanese Society for Horticultural Science, 1996, 64：741-747.
[60]Wang T, Ran Y, Atkinson RG, et al. Transformation of Actinidia eriantha：A potential species for functional genomics studies in Actinidia[J]. Plant Cell Reports, 2006, 25：425-431.
[61]Firsov AP, Dolgov SV. Agrobacterial transformation of Actinidia kolomicta[J]. Acta Horticulturae, 1997, 447：323-328.
[62]Qiu Q, Wang Z, Cai Q, et al. Changes of DHN1 expression and subcellular distribution in A. delicisoa cells under osmotic stress[J]. Science in China Series C-Life Sciences, 2002, 45(1)：1-9.
[63]朱道圩, 米银法, 陈延惠, 等. GFP基因在软枣猕猴桃愈伤组织原生质体中瞬间表达的初步研究[J]. 河南农业大学学报, 2003, 37(2)：145-148.
[64]黄宏文. 中国猕猴桃种质资源[M]. 北京：中国林业出版社, 2013.
[65]Huang HW. The Genus ACTINIDIA：A World Monograph[M]. Beijing：Science Press, 2014.
[66]Wang S, Jiang Z, Huang H, et al. Conservation and utilization of germplasm resources of the genus Actinidia[J]. Acta Horticulturae, 2003, 610：365-371.
[67]Beatson RA, Datson PM, Harris-Virgin PM, et al. Progress in the breeding of novel interspecific Actinidia hybrids[J]. Acta Horticulturae, 2007, 753：147-153.
[68]Cho HS, Jo YS, Liu IS, et al. Characteristics of Actinidia deliciosa×A. arguta and A. arguta ×A. deliciosa hybrids[J]. Acta Horticulturae, 2007, 753：205-210.
[69]王圣梅, 黄仁煌, 武显维, 等. 猕猴桃远缘杂交育种研究[J]. 果树学报, 1994, 11(1)：23-26.
[70]安和祥, 蔡达荣, 母锡金, 等. 猕猴桃种间杂交的新种质[J]. 园艺学报, 1995, 22(2)：133-137.
[71]范培格, 安和祥, 蔡达荣, 等. 美味猕猴桃海沃德与毛花猕猴桃种间杂交及优株的选育[J]. 果树学报, 2004. 21(3)：208-211.
[72]Ke SQ, Huang RH, Wang SM, et al. Studies on interspecific hybrids of Actinidia[J]. Acta Horticulturae, 1991, 297：133-139.
[73]Liang TB, Mu XJ. Observation of pollen tube behaviour and early embryogenesis following interspecies pollination between Actinidia deliciosa and A. arguta[J]. Acta Botanica Sinica, 1995, 37：607-612.
[74]Guthrie RS, Luby JJ, Bedford DS, et al. Partial dominance in Actinidia kolomikta interspecific hybrids[J]. Acta Horticulturae, 2007, 753：211-218.
[75]Mizugami T, Kim JG, Beppu K, et al. Observation of parthenocarpy in Actinidia arguta selection 'Issai'[J]. Acta Horticulturae, 2007, 753：199-204.
[76]Wu JH. Datson PM. Manako KI, et al. Meiotic chromosome pairing behaviour of natural tetraploids and induced autotetraploids of Actinidia chinensis[J]. Theoretical and Applied Genetics, 2014, 127(3)：549-557.
[77]Atkinson RG, Sharma NN, Hallett IC, et al. Actinidia eriantha：a parental species for breeding kiwifruit with novel peelability and health attributes[J]. New Zealand Journal of Forestry Science, 2009, 39：207-216.
[78]井赵斌, 魏琳, 俞靓, 等. 转录组测序及其在牧草基因资源发掘中的应用前景[J]. 草业科学, 2011b, 28(7)：1364-1369.
[79]Sun X, Liu D, Zhang X, et al. SLAF-seq：an efficient method of large-scale de novo SNP discovery and genotyping using high-throughput sequencing[J]. PLoS One, 2013, 8(3)：e58700.