ARTP诱变选育鼠李糖脂高产菌及鼠李糖脂促进枯草芽孢杆菌产纤维素酶的研究

doi:10.13560/j.cnki.biotech.bull.1985.2015.11.025

生物技术通报 ›› 2015, Vol. 31 ›› Issue (11): 195-201.doi: 10.13560/j.cnki.biotech.bull.1985.2015.11.025

ARTP诱变选育鼠李糖脂高产菌及鼠李糖脂促进枯草芽孢杆菌产纤维素酶的研究

陈利娟, 吴斌, 何冰芳

南京工业大学生物与制药工程学院，南京 211816

收稿日期:2015-03-19 出版日期:2015-11-26 发布日期:2015-11-26
作者简介:陈利娟，女，硕士，研究方向: 应用微生物；E-mail: 1045339607@qq.com
基金资助:
国家“863”计划项目(2012AA022205)，国家“973”计划项目(2011CB707405)

Screening of High-yield Rhamnolipid Producing Strain by ARTP and the Effect of Rhamnolipid on the Cellulase and Xylanase

Chen Lijuan, Wu Bin, He Bingfang

College of Biotechnology and Pharmaceutical Engineering，Nanjing Tech University，Nanjing 211816

Received:2015-03-19 Published:2015-11-26 Online:2015-11-26

摘要/Abstract

摘要： 旨在选育鼠李糖脂高产菌株，以实验室筛选的产鼠李糖脂的Pseudomonas aeruginosa C3为出发菌株进行常压室温等离子体诱变(ARTP)，选育出一株高产突变株Pseudomonas aeruginosa SC-11，产量比出发菌株提高了74.1%。进一步对产鼠李糖脂的摇瓶发酵培养基和发酵条件进行了优化，优化后的鼠李糖脂产量达42 g/L，底物转化率达到0.7 g/g底物。添加0.01%鼠李糖脂到Bacillus subtilis CL产羧甲基纤维素酶与木聚糖酶的培养基中，羧甲基纤维素酶活与木聚糖酶活分别提高12.9%和18.3%。研究表明，鼠李糖脂通过增加细胞通透性来提高胞外酶产量。

关键词: 常压室温等离子体诱变, 鼠李糖脂, 羧甲基纤维素酶, 木聚糖酶

Abstract: In order to obtain an industrial strain with higher rhamnolipid production, the original strain of Pseudomonas aeruginosa C3 was mutated by atmospheric and room temperature plasmas. A good genetic stability strain SC-11 was screened from the mutants, which produced rhamnolipid 74.1% higher than that by the original strain. Through single factor experiments, the culture medium for rhamnolipid production was opttimized. The yield of rhamnolipid could reach 42 g/L and the substrate conversion rate is 0.7 g/g substrates. 0.01% rhamnolipid could enhance CMCase by 12.9% and xylanase by 18.3%. The results show that rhamnolipid increased the production of cellulase by increasing cell permeability.

Key words: atmospheric and room temperature plasmas(ARTP), rhamnolipid, CMCase, xylanase

陈利娟, 吴斌, 何冰芳. ARTP诱变选育鼠李糖脂高产菌及鼠李糖脂促进枯草芽孢杆菌产纤维素酶的研究[J]. 生物技术通报, 2015, 31(11): 195-201.

Chen Lijuan, Wu Bin, He Bingfang. Screening of High-yield Rhamnolipid Producing Strain by ARTP and the Effect of Rhamnolipid on the Cellulase and Xylanase[J]. Biotechnology Bulletin, 2015, 31(11): 195-201.

参考文献

[1]Desai JD, Banat IM. Microbial production of surfactants and their commercial potential[J]. Microbiology and Molecular Biology Reviews, 1997, 61(1): 47-64.
[2]钱欣平, 阳永荣, 孟琴, 等. 生物表面活性剂对微生物生长和代谢的影响[J]. 微生物学通报, 2002, 29(3): 75-78.
[3]左晶, 王学川. 生物表面活性剂的应用[J]. 化学工业与工程技术, 2005, 26(2): 23-26.
[4]Nitschke M, Costa SG, Contiero VAO. Biosurfactants in food industry[J]. Biotechnology Progress, 2007, 21(3): 252-259.
[5]Banat IM, Makkar RS, Cameotra SS. Potential commercial applications of microbial surfactants[J]. Applied Microbiology and Biotechnology, 2000, 53(5): 495-508.
[6]Singh P, Cameotra SS. Potential applications of microbial surfactantsinbiomedical sciences[J]. Trends in Biotechnology, 2004, 22(3): 142-146.
[7]Wu ZB, Zhong H, Yuan XZ, et al. Adsorptive removal of methylene blue by rhamnolipid-functionalized graphene oxide from wastewater[J]. Water Research, 2014, 67(5): 330-344.
[8]金丽华, 方明月, 张翀, 等. 常压室温等离子体快速诱变产油酵母的条件及其突变株的特性[J]. 生物工程学报, 2011, 27(3): 461-467.
[9] Pinzon NM, Ju LK. Improved detection of rhamnolipid production using agar plates containing methylene blue and cetyltrimethylammo-nium bromide[J]. Biotechnology Letters, 2009, 31(3): 1583-1588.
[10]Koch AK, Kappeli O, Fiechter A, et al. Hydrocarbon assimilation and biosurfactant production in Pseudomonas aeruginosa mutants[J]. Journal of Bacteriology, 1991, 173(13): 4214-4219.
[11] Benincasa M, Contiero J, Manresa MA, et al. Rhamnolipid produc-tion by Pseudomonas aeruginosa LBI growing on soapstock as the sole carbon source[J]. Food Engineering, 2002, 54(4): 283-288.
[12]柏中中, 孙家铎, 吴斌. 基于常压室温等离子体技术诱变选育D-乳酸高发酵速率菌株[J]. 食品工业科技, 2013, 34(24): 173-176.
[13]Liu J, Yuan X, Zeng Z. Effect of biosurfactant on cellulose and xylanase production by Trichoderma viride in solid substrate fermentation[J]. Process Biochemistry, 2006, 41(11): 2347-2351.
[14]沈蔚, 杨树林, 宁长发, 等. 铜绿假单胞菌(Pseudomonas aeruginosa)BS-03的诱变育种及产鼠李糖脂类生物表面活性剂的摇瓶工艺初探[J]. 食品与发酵工业, 2004, 30(12): 26-30.
[15]秦新政, 杨新平, 唐娴, 等. 产物诱变选育鼠李糖脂高产菌株的研究[J]. 新疆农业科学, 2008, 45(6): 1060-1063.
[16]马满英, 施周, 刘有势, 等. 假单胞菌AB93066产鼠李糖脂发酵条件的优化[J]. 精细化工, 2008, 25(3): 221-225.
[17]时进钢, 袁兴中, 曾光明, 等. 鼠李糖脂生物表面活性剂及其在生活垃圾堆肥化中的应用: 中国, 1207383[P]. 2005-06-22.
[18]Giani C, Wullbrandt D, Rothert R, Meiwes J. Pseudomonas aeruginosa and its use in a process for the biotechnological preparation of L-rhamnose: United Patent, 5658793[P]. 1997-08-19.
[19]Liu XL, Zeng GM, Tang L, et al. Effects of dirhamnolipid and SDS on enzyme production from Phanerochaete chrysosporium in submerged fermentation[J]. Process Biochemistry, 2008, 43(11): 1300-1303.
[20]Wang HY, Fan BQ, Li CH. Effects of rhamnolipid on the cellulase and xylanase in hydrolysis of wheat straw[J]. Bioresource Technology, 2011, 102(11): 6515-6521.
[21]刘佳, 袁兴中, 曾光明, 等. 表面活性剂对绿色木霉产纤维素酶影响的实验研究[J]. 中国生物工程杂志, 2006, 26(8): 62-66.

ARTP诱变选育鼠李糖脂高产菌及鼠李糖脂促进枯草芽孢杆菌产纤维素酶的研究

Screening of High-yield Rhamnolipid Producing Strain by ARTP and the Effect of Rhamnolipid on the Cellulase and Xylanase

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