香菇多糖增强黄瓜幼苗对炭疽病菌抗性机理

doi:10.13560/j.cnki.biotech.bull.1985.2016.03.015

摘要/Abstract

摘要： 旨在考察香菇多糖诱导黄瓜(Cucumis sativus L. cv. Jinfeng)幼苗叶片细胞产生防御响应的效果。经0.75 g/L香菇多糖处理后, 叶片胞内H₂O₂和水杨酸浓度迅速增加, 分别在10 min和4 h达到峰值, 随后均逐渐下降至诱导前水平。经香菇多糖诱导后, 胞内抗性相关蛋白β-1, 3-葡聚糖酶、几丁质酶和苯丙氨酸氨解酶比活性均显著增加, 96 h后酶活力仍然保持较高水平。进一步研究发现, 经香菇多糖诱导后, 黄瓜对炭疽病菌(Colletotrichum orbiculare)抗性明显增强。经2.00 g/L香菇多糖处理后, 叶片菌斑面积和数量分别显著下降至对照水平的53.1%和51.3%, 结果表明香菇多糖具有诱导黄瓜细胞产生防御响应的作用。

关键词: 香菇多糖, 黄瓜, 防御响应, 炭疽病菌

Abstract: This study is to investigate the inducing effect of lentinan on the defense responses of cucumber(Cucumis sativus L. cv. Jinfeng)seedling leaves. The seedlings were treated with 0.75 g/L of lentinan. The concentration of H₂O₂ and salicylic acid in cells of leaves increased rapidly and reached the peak levels at 10 min and 4 h, respectively. Afterwards, the levels of both H₂O₂ and salicylic acid gradually declined to the ones before treatment. Moreover, the activities of defense related proteins, including β-1, 3-glucanase, chitinase, and phenylalanine ammonia lyase, significantly increased after lentinan induction, and still kept stable after 96 hours. In addition, the resistance of cucumber treated with lentinan to Colletotrichum orbiculare was notably enhanced after lentinan induction. Both the number and area of lesions on cucumber leaves significantly decreased to 53.1% and 51.3% in comparison with the control, respectively, which indicated that lentinan played a role in inducing the defense responses of cucumber cells.

Key words: lentinan, Cucumis sativus L. cv. Jinfeng, defense response, Colletotrichum orbiculare

张文华. 香菇多糖增强黄瓜幼苗对炭疽病菌抗性机理[J]. 生物技术通报, 2016, 32(3): 93-97.

ZHANG Wen-hua. The Mechanism of Lentinan Enhancing the Resistance of Cucumber Seedlings to Colletotrichum orbiculare[J]. Biotechnology Bulletin, 2016, 32(3): 93-97.

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