阿魏菇醇提物抗肿瘤功效及三萜类成分的提取

doi:10.13560/j.cnki.biotech.bull.1985.2016.07.030

摘要/Abstract

摘要： 为探究阿魏菇醇溶性物质（PFECs）抗肿瘤功效,优化阿魏菇醇提物三萜类化合物（PFTPs）提取条件,获取最大提取率。采用MTT法检测PFECs对5种肿瘤细胞生长抑制活性;对两种正常细胞无明显抑制作用;采用超声辅助、微波辅助热水浸提法、单因素试验与响应曲面法相结合,对阿魏菇三萜类化合物提取工艺优化。结果显示,PFECs具有显著的抗肿瘤活性;PFTPs提取的最佳工艺参数为：料液比1∶19（g/mL）、提取温度75℃、提取时间22 min（超声40 min后）,提取率为4.42%。

关键词: 阿魏菇, 醇提物, 活性成分, 抗肿瘤, 三萜化合物, 提取优化, 响应曲面

Abstract: This work aims to explore the antitumor activity of alcohol soluble components from Pleurotus ferulae（PFECs）,and to optimize the extraction condition of triterpenoids from P. ferulae（PFTPs）for maximum extraction yield. MTT was used to detect the inhibitory activity of PFECs to 5 kinds of tumor cells,no significant inhibition to 2 kinds of normal cells was observed. Ultrasonic-assisted,hot-water leaching with ultrasonic-assisted,single-factor experiment,and response surface methodology were jointly employed to optimize the extraction process of PFTPS. The results showed that PFECs presented significant antitumor activity. The optimal process parameters for the PFTPs extraction were：the ratio of ethanol to material 1∶19（g/mL）,extraction time 22 min and extraction temperature 7℃,and the extracting yield of PFTPs was 4.42%.

Key words: Pleurotus ferulae, ethanol extracts, active components, antitumor activity, triterpenoids, optimization of extraction, response surface methodology

王为兰,陈开旭,刘军,段魏魏,徐亚楠,张富春. 阿魏菇醇提物抗肿瘤功效及三萜类成分的提取[J]. 生物技术通报, 2016, 32(7): 206-216.

WANG Wei-lan CHEN Kai-xu LIU Jun DUAN Wei-wei XU Ya-nan ZHANG Fu-chun. Antitumor Activity of the Ethanol Extract of Pleurotus ferulae and the Extraction Procedure of Triterpenoid Components[J]. Biotechnology Bulletin, 2016, 32(7): 206-216.

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