石斛内生甲基营养芽胞杆菌的拮抗和促生作用研究

doi:10.13560/j.cnki.biotech.bull.1985.2016.08.029

摘要/Abstract

摘要： 从霍山石斛中分离内生菌,旨在获得具有广谱抗菌活性和促生作用的内生细菌。以石斛黑斑病菌为指示菌,用对峙培养法筛选具有抗菌活性的内生菌;对筛选到的具有拮抗活性的菌株进行抗菌谱和促生长潜力的测定。结果显示,从霍山石斛中分离获得22株内生细菌,其中菌株RA具有较强抑菌活性,根据16S rDNA 序列分析结果鉴定 RA为甲基营养芽孢杆菌 Bacillus methylotrophicus。菌株RA可以产生IAA、嗜铁素、蛋白酶、表面活性剂和生物膜,RA对 8 种植物病原菌也具有较好的抑制作用,具有广谱抗菌活性;并且可以显著促进玉米幼苗的生长。RA菌株具有作为生防菌剂和生物肥料应用的良好潜力。

关键词: 霍山石斛, 内生细菌, 甲基营养芽胞杆菌, 抑菌活性, 促生长

Abstract: Isolating endophytic bacteria form Dendrobium huoshanense aimed to obtain endophytic bacteria that have broad-spectrum antimicrobial activity and plant growth-promoting trait. Plate confrontation method was applied to screen the endophytic bacteria with antimicrobial activities against the black spot disease of D. huoshanense,of which the antimicrobial spectrum and plant growth-promoting（PGP）traits were determined. Total 22 endophytes were isolated from D. huoshanense,and strain RA presented strong inhibitory activities against Alternaria tenuissima and it was identified as Bacillus methylotrophicus on the basis of 16S rDNA sequence analysis and was selected for further studies. Strain RA was capable of co-producing IAA,siderophore,protease,biosurfactant,and biofilm,and significantly inhibited 8 plant pathogens and had broad-spectrum antimicrobial effects. A significant improvement in corn seedlings growth was observed when seeds of corn were bacterized prior to sowing. In conclusion,results clearly suggest that strain RA is a potential candidate for use as biocontrol agent and in biofertilizer.

Key words: Dendrobium huoshanense, endophyte, Bacillus methylotrophicus, antagonistic efficacy, growth promotion

武利勤,顾海科,王青,尚宏忠,刘桂君,包放. 石斛内生甲基营养芽胞杆菌的拮抗和促生作用研究[J]. 生物技术通报, 2016, 32(8): 200-206.

WU Li-qin ,GU Hai-ke ,WANG Qing, SHANG Hong-zhong, LIU Gui-jun ,BAO Fang. Antagonistic Efficacy and Growth-Promoting Effect of Bacillus methylotrophicus Isolated from Dendrobium huoshanense[J]. Biotechnology Bulletin, 2016, 32(8): 200-206.

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