草鱼（Ctenopharyngodon idellus）FGF21基因克隆及其表达分析

doi:10.13560/j.cnki.biotech.bull.1985.2017-0729

摘要/Abstract

摘要： 采用RT-PCR技术从草鱼肝脏中克隆了成纤维细胞生长21（FGF21）基因序列,并利用实时荧光定量PCR（qPCR）技术对该基因在草鱼幼鱼不同组织中表达进行了研究。结果表明,草鱼FGF21基因（GenBank登录号为MF_094727）cDNA序列全长615 bp,其中5'端非翻译区51 bp,开放阅读框564 bp,编码187个氨基酸。氨基酸序列同源性分析表明,草鱼FGF21基因与斑马鱼同源性最高（78.86%）,与犀角金线鲃、鲤、虹鳟、大西洋鲑、罗非鱼和日本青鱂的同源性分别为77.66%、73.94%、53.29%、52.41%、43.71%和35.96%,与人和小鼠同源性较低,分别为31.87%和30.39%。qPCR分析表明：FGF21基因在草鱼幼鱼肝脏、前肠、中肠、后肠、心脏、肌肉、肾脏和全脑中均有表达,在肌肉、前肠和中肠表达量最高,表明该基因可能在草鱼肌肉和肠道等组织发挥重要作用。

关键词: 草鱼, FGF21基因, 基因克隆, 表达分析

Abstract: In this study,the Reverse Transcription PCR（RT-PCR）was used to clone full-length sequence of FGF21（Fibroblast growth factor）gene from grass carp liver,and the quantitative real-time PCR（qPCR）was used to detect the expression levels of FGF21 gene in different juvenile tissues. Sequence analysis revealed that the full-length of cDNA of the FGF21 gene（GenBank accession number：MF_094727）was 615 bp,containing 51 bp 5'-untranslated region,564 bp open reading frame（ORF）,and encoding 187 amino acids. Alignment analysis showed that the amino acid sequence of FGF21 gene in grass carp were 78.86%,77.66%,73.94%,53.29%,52.41%,43.71%,35.96%,31.87%,and 30.39% identical to that from zebrafish（Danio rerio）,horned golden-line barbel（Sinocyclocheilus rhinocerous）,common carp（Cyprinus carpio）,rainbow trout（Oncorhynchus mykiss）,Atlantic salmon（Salmo salar）,Nile tilapia（Oreochromis niloticus）,Japanese medaka（Oryzias latipes）,human（Homo sapiens）,and house mouse（Mus musculus）,respectively. qPCR analysis indicated that the FGF21 gene expressed in all examined tissues including liver,foregut,midgut,hindgut,heart,muscle,kidney,and brain,and the highest in muscle,foregut,and midgut. These results indicate that the FGF21 gene may play an important role in muscle and intestine of grass carp.

Key words: grass carp（Ctenopharyngodon idellus）, FGF21 gene, gene clone, expression analysis

黄龙, 吴本丽, 何吉祥, 宋光同, 陈静, 汪翔, 武松. 草鱼（Ctenopharyngodon idellus）FGF21基因克隆及其表达分析[J]. 生物技术通报, 2018, 34(1): 153-159.

HUANG Long, WU Ben-li, HE Ji-xiang, SONG Guang-tong, CHEN Jing, WANG Xiang, WU Song. Cloning and Expression Analysis of FGF21 Gene in Ctenopharyngodon idellus[J]. Biotechnology Bulletin, 2018, 34(1): 153-159.

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