细菌多元基因编辑技术研究进展及其在合成生物学中的应用

doi:10.13560/j.cnki.biotech.bull.1985.2018-0089

摘要/Abstract

摘要： 多元基因编辑技术是指通过一次实验操作在同一个细胞内完成多个靶位点基因编辑的技术。高通量化是基因编辑技术发展的重要方向,而多元基因编辑的出现与发展是实现高通量基因编辑的重要中间过程。目前,细菌中多元基因编辑技术主要有三种形式并行发展,分别是基于迭代编辑、基于CRISPR/Cas9等新基因编辑工具、基于大片段基因合成组装的多元基因编辑技术。综述了3种类型的多元基因编辑技术的原理和发展,以及多元基因编辑技术在微生物合成生物学中的广泛应用前景,讨论了目前多元基因编辑技术存在的问题,并展望了多元基因编辑技术进一步实现高通量化的发展方向。

关键词: 细菌基因编辑, 多元基因编辑, 合成生物学

Abstract: Multiplex genome engineering refers to the technology that can simultaneously edit multiplex genome targets in one cell by a single experiment. High-throughput is a key direction of gene editing technologies,and the occurrence and development of multiplex genome engineering is an important intermediate stage of high-throughput genome engineering. So far there are mainly three strategies for multiplex genome engineering in bacteria,including iteration based editing,CRISPR/Cas9-based newly developed high-efficiency tools editing,and large synthetic DNA-based editing. In this article,the principles and developments of these three kinds of multiplex genome engineering technologies,as well as their applications in synthetic biology of microorganism are reviewed,and the shortcoming of these technologies are discussed. Besides,we propose the future development directions of multiplex genome engineering.

Key words: bacterial genome engineering, multiplex genome engineering, synthetic biology

郭明璋,刘海燕,杜若曦,许文涛. 细菌多元基因编辑技术研究进展及其在合成生物学中的应用[J]. 生物技术通报, 2018, 34(5): 22-31.

GUO Ming-zhang, LIU Hai-yan, DU Ruo-xi, XU Wen-tao. Advances in Bacterial Multiplex Genome Engineering and Its Applications in Synthetic Biology[J]. Biotechnology Bulletin, 2018, 34(5): 22-31.

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