拟南芥热形态建成中PIF4下游基因研究

doi:10.13560/j.cnki.biotech.bull.1985.2018-0211

生物技术通报 ›› 2018, Vol. 34 ›› Issue (7): 57-65.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0211

拟南芥热形态建成中PIF4下游基因研究

汪硕¹, 丁岚¹, 刘建祥^1,2, 韩佳嘉^1,2

1. 复旦大学生命科学学院,上海 200433;
2. 浙江大学生命科学学院,杭州 310027

收稿日期:2018-03-13 出版日期:2018-07-26 发布日期:2018-08-01
作者简介:汪硕,女,硕士研究生,研究方向：遗传学;E-mail：11307110294@fudan.edu.cn
基金资助:
国家自然科学基金项目（31625004）,中国博士后基金（2015M571482）,中央高校基本科研业务费专项资金（2018QNA6001）

PIF4-Regulated Thermo-responsive Genes in Arabidopsis

WANG Shuo¹, DING Lan¹, LIU Jian-xiang^1,2, HAN Jia-jia²

1. School of Life Sciences,Fudan University,Shanghai 200433;
2. College of Life Sciences,Zhejiang University,Hangzhou 310027

Received:2018-03-13 Published:2018-07-26 Online:2018-08-01

摘要/Abstract

摘要： 植物细胞伸长是植物生长和形态建成的主要方式之一,受到激素和环境信号等许多因素调控,而光敏色素PHY B互作蛋白PIF4可以促进植物在环境温度升高后的形态建成即热形态建成,但PIF4在这个响应过程中全基因组水平调控的下游基因未见报道。本研究进一步通过遗传实验证明PIF4在热形态建成中发挥着重要的作用。通过RNA-Seq实验分析得到热形态建成中248个PIF4依赖的和21个PIF4部分依赖的下游基因,包括生长素信号通路和非生物逆境响应基因。结合已有CHIP-Seq数据得到74个热形态建成中PIF4结合靶标基因。拟南芥热形态建成过程中PIF4下游基因的研究为更好理解植物对环境温度升高的响应奠定了基础。

关键词: 环境温度升高, 下胚轴伸长, PIF4, 下游基因, RNA-Seq, ChIP-qPCR

Abstract: Plant growth and development programs are coordinated with various internal factors such as phytohormones and environmental signals such as fluctuated temperatures. The Phytochrome B（PHYB）interacting protein PIF4 promotes plant morphogenesis at elevated ambient temperature,however,genome-wide downstream genes of PIF4 during this response have not been reported. In this study,we confirmed the important role of PIF4 in thermoresponsive morphogenesis in Arabidopsis,and 248 PIF4-dependent and 21 PIF4 partially dependent downstream genes at elevated ambient temperature were further revealed by RNA-Seq,including those genes in auxin responsive pathway. 74 possible direct targets of PIF4 in thermomorphogenesis were identified by combining the previous published CHIP-Seq data and our RNA-Seq data,and 6 out of 7 were confirmed by ChIP-qPCR. Thus,our study has revealed PIF4 downstream genes during thermomorphogenesis,which are important for a better understanding of plant responses to elevated ambient temperature.

Key words: ambient high temperature, hypocotyl elongation, PIF4, downstream gene, RNA-Seq, ChIP-qPCR

汪硕, 丁岚, 刘建祥, 韩佳嘉. 拟南芥热形态建成中PIF4下游基因研究[J]. 生物技术通报, 2018, 34(7): 57-65.

WANG Shuo, DING Lan, LIU Jian-xiang, HAN Jia-jia. PIF4-Regulated Thermo-responsive Genes in Arabidopsis[J]. Biotechnology Bulletin, 2018, 34(7): 57-65.

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拟南芥热形态建成中PIF4下游基因研究

PIF4-Regulated Thermo-responsive Genes in Arabidopsis

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