沙冬青属植物响应非生物胁迫的蛋白质组学研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2018-0780

摘要/Abstract

摘要： 沙冬青属植物具有抗寒、抗旱、抗盐碱等特性,是研究植物逆境胁迫和筛选天然抗逆基因库的理想材料。非生物胁迫是限制沙冬青属植物生长发育及地理分布的重要因素,研究沙冬青属植物响应非生物胁迫的蛋白质组学为发掘其相关抗逆蛋白质及探索抗逆机理奠定基础。通过对近年来国内外利用蛋白质组学技术研究沙冬青属植物应答逆境胁迫的相关成果进行总结归纳,综述沙冬青属植物对低温、干旱、高盐等非生物胁迫响应的蛋白质组学最新研究进展,探讨在非生物胁迫下沙冬青属植物蛋白质水平的动态变化,揭示特定的蛋白质网络以及相关逆境应答机制,并对蛋白质组学技术应用前景进行展望,以期为沙冬青属植物抗逆分子机制更深入、全面的研究提供参考依据。

关键词: 沙冬青属, 蛋白质组学, 非生物胁迫, 抗逆机制

Abstract: Ammopiptanthus is one kind of rare relic plants，showing many unique features of stress resistance and thriving in many extreme environments，such as draught stress，cold stress，high salinity stress and so on. Many studies have been proven that Ammopiptanthus is an ideal model material for studying plant resistance mechanisms as well as screening natural stress-resistant gene. Abiotic stress is one of two major factors which limits the growth and the geographical distribution of Ammopiptanthus. Studying proteomics of Ammopiptanthus in response to abiotic stress may lay a foundation for discovering stress-resistant protein，mapping stress-resistant interactive protein network mapping，and exploring stress resistance mechanism. This review summarizes the recent advances in proteomics investigation for Ammopiptanthus in response to abiotic stresses，such as low temperature，drought，and salinity，focusing on different physiological statuses of Ammopiptanthusduring abiotic stresses，to reveal protein dynamics，specific protein interactive network，and relevant stress response mechanisms. The application prospect of proteomics is also viewed here to provide a reference for in-depth and comprehensive study on the resistance molecular mechanism of Ammopiptanthus.

Key words: Ammopiptanthus, proteomics, abiotic stress, resistance mechanism

兰玉婷, 王双蕾, 李征珍, 冯金朝, 王晓东, 石莎. 沙冬青属植物响应非生物胁迫的蛋白质组学研究进展[J]. 生物技术通报, 2019, 35(1): 112-119.

LAN Yu-ting, WANG Shuang-Lei, LI Zheng-zhen, FENG Jin-chao, WANG Xiao-dong, SHI Sha. Research Advances in Proteomics of Ammopiptanthus in Responses to Abiotic Stresses[J]. Biotechnology Bulletin, 2019, 35(1): 112-119.

参考文献

[1] 王有德, 何全发, 王兴东, 等. 天然沙冬青生长、更新状况调研及利用前景探讨[J]. 宁夏农林科技, 2004(3):28-31.
[2] 傅立国. 中国植物红皮书:稀有濒危植物. 第一册[M]. 北京:科学出版社, 1991.
[3] 苏志豪, 师玮, 卓立, 等. 沙冬青(Ammopiptanthus)的遗传结构与保育[J]. 2018(1):163-171.
[4] 李慧卿, 马文元, 李慧勇. 沙冬青抗逆性及开发利用前景分析研究[J]. 世界林业研究, 2000, 13(5):67-71.
[5] Huang G, Ma S, Bai L, et al.Signal transduction during cold, salt, and drought stresses in plants[J]. Molecular Biology Reports, 2012, 39(2):969-987.
[6] Wasinger VC, Cordwell SJ, Cerpa-Poljak A, et al.Progress with gene-product mapping of the Mollicutes:Mycoplasma genitalium[J]. Electrophoresis, 1995, 16(7):1090-1094.
[7] 王兴华, 王光耀, Keong TK, 等. 蛋白质组学研究的原理、技术与应用[J]. 智慧健康, 2016, 2(4):7-12.
[8] Xia F, Yao X, Tang W, et al.Isobaric tags for relative and absolute quantitation(iTRAQ)-based proteomic analysis of hugan qingzhi and its protective properties against free fatty acid-induced L02 hepatocyte injury[J]. Frontiers in Pharmacology, 2017, 8:99.
[9] Wang X, Chen G, Liu H, et al.Four-dimensional orthogonal electrophoresis system for screening protein complexes and protein-protein interactions combined with mass spectrometry[J]. Journal of Proteome Research, 2010, 9(10):5325-5334.
[10] Liu Q, Zheng J, Sun W, et al.A proximity-tagging system to identify membrane protein-protein interactions[J]. Nature Methods, 2018, 15(9):715-722.
[11] Sriram R, Sahni AK, Dudhat VL, et al.Matrix-assisted Laser desorp-tion/ionization-time of flight mass spectrometry(MALDI-TOF MS)for rapid identification of Mycobacterium abscessus[J]. Medical Journal Armed Forces India, 2018, 74(1):22-27.
[12] Wu M, Sun R, Wang M, et al.Analysis of perfluorinated compounds in human serum from the general population in Shanghai by Liquid chromatography-tandem mass spectrometry(LC-MS/MS)[J]. Chemosphere, 2017, 168:100-105.
[13] Kosová K, Vítámvás P, Urban MO, et al.Plant abiotic stress proteomics:The major factors determining alterations in cellular proteome[J]. Frontiers in Plant Science, 2018, 9:122.
[14] Kosová K, Vítámvás P, Prášil IT, et al.Plant proteome changes under abiotic stress-contribution of proteomics studies to understanding plant stress response[J]. Journal of Proteomics, 2011, 74(8):1301-1322.
[15] Fei G, Ning W, Li H, et al.Identification of drought-responsive microRNAs and their targets in Ammopiptanthus mongolicus by using high-throughput sequencing[J]. Sci Rep, 2016, 6:34601.
[16] Tao P, Ye C, Xia X, et al.De novo sequencing and transcriptome analysis of the desert shrub, Ammopiptanthus mongolicus, during cold acclimation using Illumina/Solexa[J]. BMC Genomics, 2013, 14(1):488.
[17] Wang W, Vinocur B, Altman A.Plant responses to drought, salinity and extreme temperatures:towards genetic engineering for stress tolerance[J]. Planta, 2003, 218(1):1-14.
[18] Cao P, Song J, et al.Characterization of multiple cold induced genes from Ammopiptanthus mongolicus and functional analyses of gene AmEBP1[J]. Plant Mol Biol, 2009, 69(5):529-539.
[19] Lu C, Yin L, Li K.Proteome expression patterns in the stress tolerant evergreen Ammopiptanthus nanus under conditions of extreme cold[J]. Plant Growth Regulation, 2010, 62(1):65-70.
[20] Chory J, Wu D.Weaving the complex web of signal transduction[J]. Plant Physiol, 2001, 125(1):77-80.
[21] Kazuoka T, Oeda K. heat-stable COR(cold-regulated)proteins associated with frezzing tolerance in spinach[J]. Plant & Cell Physiology, 1992, 33(7):1107-1114.
[22] Houde M, Danyluk J, Laliberté J-F, et al.Cloning, characterization, and expression of a cDNA encoding a 50-kilodalton protein specifically induced by cold acclimation in wheat[J]. Plant Physiol, 1992, 99(4):1381-1387.
[23] Fei Y, Sun L, Huang T, et al.Isolation and identification of antifreeze protein with high activity in Ammopiptanthus mongolicus[J]. Acta Botanica Sinica, 1994, 36(8):649-650.
[24] 费云标, 魏令波, 等. 沙冬青抗冻蛋白的分离、纯化及其理化特性分析[J]. 科学通报, 2000, 45(20):2185-2189.
[25] Wei L, Jiang Y, Shu N, et al.Biological characterization of heat-stable antifreeze proteins from Leaves of Ammopiptanthus mongolicus[J]. Acta Botanica Sinica, 1999, 41(8):837-841.
[26] 祭美菊, 安黎哲, 陈拓, 等. 天山寒区冰缘植物珠芽蓼叶片抗冻蛋白的发现[J]. 2001, 23(4):342-345.
[27] 周晓蕾, 陈滔滔, 王保怀, 等. 沙冬青抗冻蛋白热滞活性的DSC研究[J]. 物理化学学报, 2001, 17(1):66-69.
[28] 师静, 等. 沙冬青胚胎晚期发生丰富蛋白基因序列及表达特性分析[J]. 北京林业大学学报, 2012, 34(4):114-119.
[29] 王红蕾, 王艳萍, 等. 沙冬青AmCSDP基因特性及转基因烟草的抗寒性分析[J]. 园艺学报, 2017, 44(4):712-722.
[30] 宋祥春, 赵惠新, 等. 低温对两种沙冬青幼苗光合生理指标的影响[J]. 新疆大学学报:自然科学版, 2009, 26(3):342-346.
[31] 郁万文, 曹福亮, 汪贵斌. 低温胁迫下银杏活性氧代谢与膜伤害的关系[J]. 东北林业大学学报, 2010, 38(7):46-48.
[32] 刘家琼, 邱明新. 沙冬青植物群落研究[J]. 中国沙漠, 1995, 15(2):109-115.
[33] Rovedahoyos G, Fonsecamoreno LP.Proteomics:a tool for the study of plant response to abiotic stress[J]. Agronomía Colombiana, 2011, 29(2):412-422.
[34] 何芳兰, 韩生慧, 等. 土壤水分对沙冬青幼苗生长及其光合荧光参数的影响[J]. 中国农学通报, 2014, 30(16):62-66.
[35] 付晨熙, 肖自华, 高飞, 等. 干旱胁迫下蒙古沙冬青叶片蛋白质组学研究[J]. 生物技术通报, 2017, 33(6):69-80.
[36] 刘家琼, 周湘红. 几种固沙植物过氧化物酶、过氧化氢酶活性及其同工酶的初步分析[M]. 兰州:甘肃科技出版社, 1993.
[37] 白娟, 龚春梅, 王刚, 等. 干旱胁迫下荒漠植物红砂叶片抗氧化特性[J]. 2010, 30(12):2444-2450.
[38] Riazi A, Matsuda K, Arslan A.Water-stress induced changes in concentrations of proline and other solutes in growing regions of young barley leaves[J]. J Exp Bot, 1985, 36(172):1716-1725.
[39] 李慧卿, 马文元. 沙生植物抗旱性比较的主要指标及分析方法[J]. 干旱区研究, 1998(4):12-15.
[40] 罗青红, 宁虎森, 何苗, 等. 5种沙地灌木对干旱胁迫的生理生态响应[J]. 林业科学, 2017, 53(11):29-42.
[41] 夏晗, 黄金生. 低温、干旱和盐胁迫下沙冬青幼苗脯氨酸含量的变化[J]. 吉林林业科技, 2007, 36(4):1-2.
[42] 岳光振, 金曼, 李俊林, 等. 沙冬青脯氨酸转运体基因的克隆及表达分析[J]. 生物技术通报, 2015, 31(5):106-112.
[43] 颜华, 贾良辉, 王根轩. 植物水分胁迫诱导蛋白的研究进展[J]. 生命的化学, 2002, 22(2):165-168.
[44] 林秀琴, 袁坤, 王真辉, 等. 植物响应逆境胁迫的比较蛋白质组学研究进展[J]. 热带农业科学, 2009, 29(2):52-57.
[45] Guo L, Yu Y, Xia X, et al.Identification and functional characterisation of the promoter of the calcium sensor gene CBl1 from the xerophyte Ammopiptanthus mongolicus[J]. BMC Plant Biology, 2010, 10:18.
[46] 丁志强, 尚桂军, 李娜, 等. 沙冬青CBl1蛋白纯化及性质研究[J]. 中国生物工程杂志, 2011, 31(2):23-29.
[47] 张美, 张会. 胚胎发育晚期丰富蛋白(LEA蛋白)与植物抗逆性研究进展[J]. 生物资源, 2017, 39(3):155-161.
[48] Sun H, Xia B, Wang X, et al.Quantitative phosphoproteomic analysis provides insight into the response to short-term drought stress in Ammopiptanthus mongolicus roots[J]. International Journal Molecular Sciences, 2017, 18(10):2158.
[49] 智冠华, 史军娜, 赵晓鑫, 等. 转沙冬青锌指蛋白基因AmZFPG烟草非生物胁迫抗性分析[J]. 园艺学报, 2013, 40(4):713-723.
[50] Wang J, Xia X, Wang J, et al.Stress responsive zinc-finger protein gene of Populus euphratica in tobacco enhances salt tolerance[J]. J Integr Plant Biol, 2008, 50(1):56-61.
[51] Wei Q, Guo Y, Cao H, et al.Cloning and characterization of an AtNHX2 -like Na + /H + antiporter gene from Ammopiptanthus mongolicus(Leguminosae)and its ectopic expression enhanced drought and salt tolerance in Arabidopsis thaliana[J]. Plant Cell Tissue & Organ Culture, 2011, 105(3):309-316.
[52] 李婧男, 刘强, 贾志宽, 等. 盐胁迫对沙冬青幼苗生长与生理特性的影响[J]. 植物研究, 2009, 29(5):553-558.