两种地衣共生藻对铜和锌的耐受性及吸附特性研究

doi:10.13560/j.cnki.biotech.bull.1985.2019-0018

摘要/Abstract

摘要： 重金属污染生物修复中,藻类对重金属的吸附潜力引起诸多研究者的关注。为了探讨从地衣体分离培养的地衣共生藻对重金属Cu²⁺、Zn²⁺吸附特性及其耐受性的差异,以2种地衣共生藻为研究材料,采用Evan’s blue染色法、BCO和双硫腙分光光度法测定地衣共生藻细胞活率,培养液及藻体内的Cu²⁺、Zn²⁺含量。结果显示：不同浓度Cu²⁺、Zn²⁺胁迫下,2种地衣共生藻的细胞活力及重金属吸附特性有所差异,对Cu²⁺胁迫的耐受性及吸附性为：P.E>B;Zn²⁺胁迫下,培养至9d时耐受为：P.E > B,随着培养时间的延长P.E的细胞活力急剧下降并低于B,但吸附率还是处于P.E > B;并且2种地衣共生藻对Zn²⁺胁迫的耐性及吸附性明显高于Cu²⁺。研究发现,来自菌藻共生的特殊生物-地卷属地衣的两种藻类比一些自由生长的藻类对铜和锌胁迫具有较高的耐受性及吸附性。

关键词: 地衣共生藻, 细胞存活率, 重金属胁迫, 耐受性, 富集作用

Abstract: In the bioremediation of heavy metal pollution,the potential of algae absorbing heavy metals has attracted the attentions of many researchers. In order to investigate the difference in adsorption and tolerance of photobiont isolated from lichen body to heavy metals Cu²⁺and Zn²⁺,2 photobionts（B and P.E）were used as research materials,Evan’s blue staining method,BCO and dithizone spectrophotometric method were to determine the cell viability of photobiont,as well as the Cu²⁺ and Zn²⁺ content in culture medium and algae. The results showed that the cell viability and heavy metal adsorption of the 2 photobionts differed under different concentrations of Cu²⁺ and Zn²⁺. Tolerance to Cu²⁺ stress and adsorption was as following P.E > B. Under Zn²⁺ stress,the tolerance after 9 d of culture was P.E > B;as the culture time prolonged,the cell viability of P.E decreased sharply and was lower than that of B,but the adsorption rate was still at P.E > B. Moreover,the tolerance and adsorption of 2 photobionts to Zn²⁺ stress were significantly higher than Cu²⁺ stress. The study found that the adsorptions of 2 photobiont to Zn²⁺ stress were significantly higher than that to Cu²⁺ stress. The study found that the two algae species of Peltigera lichen,special organisms from the symbiosis of bacteria and algae-,had higher tolerance and adsorption to Cu²⁺and Zn²⁺ stress than some freely-growing algae.

Key words: photobiont, vitality, heavy metal stress, tolerance, adsorption

吉米拉木·加马力, 美合日班·阿不力米提, 古海尼沙·买买提, 艾尼瓦尔·吐米尔. 两种地衣共生藻对铜和锌的耐受性及吸附特性研究[J]. 生物技术通报, 2019, 35(6): 69-75.

Jimilamu JIAMALI, Miheriban ABILIMITI, Guhainisha MAIMAITI, Ainiwaer TUMIER. Tolerance and Adsorption of 2 Photobionts to Heavy Metal Cu and Zn[J]. Biotechnology Bulletin, 2019, 35(6): 69-75.

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