生物技术通报 ›› 2023, Vol. 39 ›› Issue (11): 123-136.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0750

• 综述与专论 • 上一篇    下一篇

基于转运蛋白工程提升微生物菌株耐受性和生物制造效率的研究进展

李昕悦1(), 周明海1, 樊亚超2, 廖莎2, 张风丽1, 刘晨光1, 孙悦3, 张霖2, 赵心清1()   

  1. 1.微生物代谢国家重点实验室 教育部代谢与发育科学国际合作联合实验室 上海交通大学生命科学技术学院,上海 200240
    2.中石化(大连)石油化工研究院有限公司,大连 116045
    3.宁夏大学葡萄酒与园艺学院,银川 750021
  • 收稿日期:2023-08-09 出版日期:2023-11-26 发布日期:2023-12-20
  • 通讯作者: 赵心清,女,博士,教授,研究方向:微生物合成生物学及酶和化学品生产;E-mail: xqzhao@sjtu.edu.cn
  • 作者简介:李昕悦,男,博士研究生,研究方向:微物代谢工程;E-mail: lixinyue123@sjtu.edu.cn
  • 基金资助:
    国家重点研发计划(2022YFE0108500)

Research Progress in the Improvement of Microbial Strain Tolerance and Efficiency of Biological Manufacturing Based on Transporter Engineering

LI Xin-yue1(), ZHOU Ming-hai1, FAN Ya-chao2, LIAO Sha2, ZHANG Feng-li1, LIU Chen-guang1, SUN Yue3, ZHANG Lin2, ZHAO Xin-qing1()   

  1. 1. School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Joint International Research Laboratory of Metabolic & Developmental Sciences, State Key Laboratory of Microbial Metabolism, Shanghai 200240
    2. SINOPEC Dalian Research Institute of Petroleum and Petrochemicals Co., Ltd., Dalian 116045
    3. College of Enology and Horticulture, Ningxia University, Yinchuan 750021
  • Received:2023-08-09 Published:2023-11-26 Online:2023-12-20

摘要:

微生物细胞工厂广泛用于生物燃料以及高值化学品和大宗化学品的可持续生产,但是高浓度产物和底物以及多种环境胁迫条件会抑制菌株的发酵效率,降低生产的经济性。因此,增强菌株耐受性对于目的产物的高效和可持续生产至关重要。近年来,利用转运蛋白工程保护菌株免受毒性化合物的损害以提升菌株耐受性的策略日益受到研究者的关注。因此,本文总结了基于微生物转运蛋白工程改造提升菌株耐受性的研究进展,分析了目前微生物转运蛋白研究领域中存在的关键问题,并探讨了基于转运蛋白工程提升微生物菌株耐受性的策略,尤其对人工智能在转运蛋白功能注释、结构模拟和底物-转运蛋白互作预测中的应用进行了总结和展望,以期能够促进微生物在绿色生物制造领域的应用。

关键词: 绿色生物制造, 转运蛋白, 胁迫耐受性, 工业微生物, 发酵效率

Abstract:

Microbial cell factories have been extensively used for sustainable production of biofuels, as well as high value and bulk chemicals. However, high concentration products or substrates, as well as stressful conditions during industrial production, may compromise fermentation efficiency and decreasing economics of production. In this context, microbial stress tolerance is crucial for green and sustainable production of the target products. In recent years, the use of transporters to protect microbial cells from toxic compounds for enhancing strain tolerance has received increasing worldwide attention. This review summarizes the progress of studies on microbial strain tolerance enhancement based on transporter engineering, analyses the current key points in the field of transporter research and discusses strategies to enhance strain tolerance based on transporter manipulation. Especially,the review highlights the applications of artificial intelligence in transporter annotation, structure simulation and substrate-transporter interaction prediction, aiming to promote the application of microorganisms in biological manufacturing.

Key words: green biological manufacturing, membrane transporter, stress tolerance, industrial microorganism, fermentation efficiency