马铃薯StSRP1的克隆、表达及生物信息学分析

doi:10.13560/j.cnki.biotech.bull.1985.2019-0029

摘要/Abstract

摘要： 胁迫相关基因的表达导致代谢物的积累和生化与生理途径的改变,对于植物适应不利胁迫条件至关重要。通过研究马铃薯StSRP1的结构和在逆境胁迫中的表达情况,为验证马铃薯StSRP1在抵抗生物胁迫过程中的作用奠定了基础。运用电子克隆法获得StSRP1全长序列,利用生物信息学分析StSRP1的核酸序列、氨基酸序列、蛋白质结构和启动子,基于NCBI数据库,选取20条与StSRP1匹配度高的胁迫相关蛋白序列构建了系统进化树,对不同物种间的SRP蛋白进行比对。经ABA、MJ和病菌的诱导,对StSRP1进行qRT-PCR分析。克隆获得StSRP1全长867 bp,可编码288个氨基酸,无信号肽序列,无跨膜区,有34个可能的磷酸化位点,亲水性蛋白,定位于内质网。StSRP1的表达受ABA、MJ和病菌的调控。StSRP1可能参与马铃薯逆境胁迫应答过程

关键词: 马铃薯, 胁迫相关基因, 生物信息学, 实时荧光定量PCR

Abstract: The expression of stress-related genes（StSRP1）leads to the accumulation of metabolites and changes in biochemical and physiological pathways,which are essential for plants to adapt to adverse stress conditions. By studying the structure of potato StSRP1 and its expression in stress,the foundation for verifying the role of potato StSRP1 in the process of resistance to biological stress is laid. Electronic cloning was used to clone the full-length sequence of StSRP1 gene,bioinformatics was to analyze the nucleic acid sequence,amino acid sequence,protein structure and promoter of StSRP1. Based on the NCBI database,20 strains of stress-related protein sequences highly matched with StSRP1 were selected to construct a phylogenetic tree,and SRP proteins among different species were compared. StSRP1 induced by ABA,MJ and pathogen was analyzed by qRT-PCR. The full length of the cloned sequence was 867 bp,encoding 288 amino acids,no signal peptide sequence,no transmembrane region,34 possible phosphorylation sites,hydrophilic protein,and localized to the endoplasmic reticulum. In sum,the expression of StSRP1 is regulated by ABA,MJ and pathogens. StSRP1 may be involved in the potato stress response process.

Key words: potato, stress-related protein, bioinformatics, qRT-PCR

庞鹏湘, 常燕楠, 尉瑞敏, 郜刚. 马铃薯StSRP1的克隆、表达及生物信息学分析[J]. 生物技术通报, 2019, 35(7): 10-16.

PANG Peng-xiang, CHANG Yan-nan, YU Rui-min, GAO Gang. Cloning,Expression and Bioinformatics Analyses of StSRP1 Gene in Solanum tuberosum[J]. Biotechnology Bulletin, 2019, 35(7): 10-16.

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